Information on EC 1.14.13.138 - indolin-2-one monooxygenase

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The expected taxonomic range for this enzyme is: Zea mays

EC NUMBER
COMMENTARY hide
1.14.13.138
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RECOMMENDED NAME
GeneOntology No.
indolin-2-one monooxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
indolin-2-one + NAD(P)H + H+ + O2 = 3-hydroxyindolin-2-one + NAD(P)+ + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoxazinoid biosynthesis
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Biosynthesis of secondary metabolites
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DIBOA-glucoside biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
indolin-2-one,NAD(P)H:oxygen oxidoreductase (3-hydroxylating)
The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses). It is a member of the cytochrome P-450 dependent monooxygenases.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzoxazin-3-one + NADPH + H+ + O2
?
show the reaction diagram
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?
indolin-2-one + NADPH + H+ + O2
3-hydroxyindolin-2-one + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
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NADPH
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.018
benzoxazin-3-one
0.01 - 0.017
indolin-2-one
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00194
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recombinant enzyme, using indolin-2-one as substrate, at pH 7.5 and 25C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
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expressed in Saccharomyces cerevisiae WAT11 cells