Information on EC 1.14.13.115 - angelicin synthase

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The expected taxonomic range for this enzyme is: Pastinaca sativa

EC NUMBER
COMMENTARY hide
1.14.13.115
-
RECOMMENDED NAME
GeneOntology No.
angelicin synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-columbianetin + NADPH + H+ + O2 = angelicin + NADP+ + acetone + 2 H2O
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(+)-columbianetin,NADPH:oxygen oxidoreductase
This P-450 monooxygenase enzyme is involved in the formation of angular furanocoumarins. Attacks its substrate by syn-elimination of hydrogen from C-3'.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
fragment
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-columbianetin + NADPH + H+ + O2
angelicin + NADP+ + acetone + 2 H2O
show the reaction diagram
syn-[3'D]columbianetin + NADPH + O2 + H+
syn-[3'D](anti-3'-hydroxy)-columbianetin + NADP+ + H2O
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-columbianetin + NADPH + H+ + O2
angelicin + NADP+ + acetone + 2 H2O
show the reaction diagram
C0SJS3
the enzyme is involved in angular furanocoumarin pathway
negligible amounts of a hydroxylated columbianetin by-product
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014 - 0.0021
(+)-columbianetin
0.0014 - 0.0038
syn-[3'D]columbianetin
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.32 - 1.9
(+)-columbianetin
0.06 - 3.9
syn-[3'D]columbianetin
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
227 - 920
(+)-columbianetin
11732
30 - 967
syn-[3'D]columbianetin
10729
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in yeast