Information on EC 1.14.13.110 - geranylgeraniol 18-hydroxylase

Word Map on EC 1.14.13.110
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.13.110
-
RECOMMENDED NAME
GeneOntology No.
geranylgeraniol 18-hydroxylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeraniol + NADPH + H+ + O2 = 18-hydroxygeranylgeraniol + NADP+ + H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Diterpenoid biosynthesis
-
-
plaunotol biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
geranylgeraniol,NADPH:oxygen oxidoreductase (18-hydroxylating)
A heme-thiolate protein (P-450).
CAS REGISTRY NUMBER
COMMENTARY hide
209915-72-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Ohba
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Vitis vinifera x Vitis vinifera
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeraniol + NADH + H+ + O2
18-hydroxygeranylgeraniol + NAD+ + H2O
show the reaction diagram
-
NADH shows about 70% of the activity with NADPH
-
-
?
geranylgeraniol + NADPH + H+ + O2
18-hydroxygeranylgeraniol + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeraniol + NADPH + H+ + O2
18-hydroxygeranylgeraniol + NADP+ + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
NADH shows about 70% of the activity with NADPH
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
improvement of thin-layer chromatography for enzyme assay of geranylgeraniol 18-hydroxylase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
above and below the pH optimum (pH 5) activity declines rapidly and is undetectable after 60 min
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
activity in the heated cell-free extract increases steadily during the first 30 min of the treatment. Thereafter, the enzyme activity declines rapidly and is almost undetectable after 60 min