Information on EC 1.14.12.9 - 4-chlorophenylacetate 3,4-dioxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: unclassified pseudomonads

EC NUMBER
COMMENTARY hide
1.14.12.9
-
RECOMMENDED NAME
GeneOntology No.
4-chlorophenylacetate 3,4-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-chlorophenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + chloride + NAD+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
-
-
Tyrosine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-chlorophenylacetate,NADH:oxygen oxidoreductase (3,4-hydroxylating, dechlorinating)
A system, containing a reductase and an iron-sulfur oxygenase, and no independent ferredoxin. Requires Fe2+. Also acts on 4-bromophenyl acetate.
CAS REGISTRY NUMBER
COMMENTARY hide
105006-00-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CBS 3
-
-
Manually annotated by BRENDA team
strain CBS 3
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD+
show the reaction diagram
4-bromophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Br- + NAD+
show the reaction diagram
4-chlorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
show the reaction diagram
4-fluorophenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + F- + NAD+
show the reaction diagram
4-hydroxyphenylacetate + NADH + O2 + H2O
3,4-dihydroxyphenylacetate + OH- + NAD+
show the reaction diagram
-
4% of the activity of 4-chlorophenylacetate
-
-
?
phenylacetate + NADH + O2 + H2O
?
show the reaction diagram
-
6% of the activity of 4-chlorophenylacetate
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
flavoprotein, contains 1 mol of FMN per mol of reductase, no increase of activity by addition of exogenous FMN
NADPH
-
30% as effective as NADH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
1 mM, 34% inhibition
EDTA
-
1 mM, 32% inhibition
iodoacetate
-
0.01 mM, 77% inhibition
KCN
-
1 mM, 52% inhibition
NEM
-
1 mM, 38% inhibition
NH4+
-
inhibition of activity of reductase component
PCMB
-
0.005 mM, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0063
2,6-dichlorophenolindophenol
-
-
0.0023
cytochrome c
-
-
0.032
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.144
-
dioxygenase component of of 4-chlorophenylacetate 3,4-dioxygenase
30
-
reductase component of of 4-chlorophenylacetate 3,4-dioxygenase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
reductase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration
140000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration
144000
-
dioxygenase component of 4-chlorophenylacetate 3,4-dioxygenase, ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
trimer
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.5
-
4°C, 10 days, 50% loss of activity
439043
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
3 min, 50% loss of activity
55
-
90-100% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
75% loss of activity after 16 h dialysis
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
reductase component loses activity in presence of molecular oxygen
-
439043
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM potassium phosphate buffer, pH 6.0-7.5, 10 days, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dioxygenase component
-
reductase component
-