Information on EC 1.14.12.15 - terephthalate 1,2-dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.12.15
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RECOMMENDED NAME
GeneOntology No.
terephthalate 1,2-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
terephthalate + NADH + H+ + O2 = (1R,6S)-dihydroxycyclohexa-2,4-diene-1,4-dicarboxylate + NAD+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Microbial metabolism in diverse environments
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Polycyclic aromatic hydrocarbon degradation
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terephthalate degradation
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SYSTEMATIC NAME
IUBMB Comments
benzene-1,4-dicarboxylate,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Has been shown to contain a Rieske [2Fe-2S] cluster
CAS REGISTRY NUMBER
COMMENTARY hide
162032-76-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain E6
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Manually annotated by BRENDA team
strain E6
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Manually annotated by BRENDA team
strain IAM 1152
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Manually annotated by BRENDA team
strain T-2
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Manually annotated by BRENDA team
strain T7
SwissProt
Manually annotated by BRENDA team
strain T7
SwissProt
Manually annotated by BRENDA team
strain DK17
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Manually annotated by BRENDA team
strain DK17
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-Dicarboxynaphthalene + O2
?
show the reaction diagram
2,5-Dicarboxypyridine + O2
?
show the reaction diagram
terephthalate + NAD(P)H + O2 + H+
(1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD(P)+
show the reaction diagram
terephthalate + NADH + O2 + H+
(1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
terephthalate + NADH + O2 + H+
(1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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TphA1II contains 0.7 mol of FAD/mol of protein, it binds one FAD molecule per monomer
iron-sulfur centre
[2Fe-2S] Rieske center
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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the enzyme is a Rieske [2Fe-2S] protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.051
NADH
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-
0.01
NADPH
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0.072
terephthalate
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at 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.8
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
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oxygenase component of the TER dioxygenase system
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 36
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oxygenase component of the TER dioxygenase system
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
isoelectric focusing, beta subunit
5.3
isoelectric focusing, alpha subunit
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17236
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2 * 48000, SDS-PAGE, TphA2II + 2 * 17000, SDS-PAGE, TphA3II. 2 * 48763, sequence analysis, TphA2II + 2 * 17236, sequence analysis, TphA3II
18000
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2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE
33000
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subunit TphBII, SDS-PAGE
37000
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1 * 37000, SDS-PAGE, TphA1II. 1 x 38861, sequence analysis, TphA1II
40000
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gel filtration, TphA1II
46000
alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE
48000
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2 * 48000, SDS-PAGE, TphA2II + 2 * 17000, SDS-PAGE, TphA3II. 2 * 48763, sequence analysis, TphA2II + 2 * 17236, sequence analysis, TphA3II
48763
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2 * 48000, SDS-PAGE, TphA2II + 2 * 17000, SDS-PAGE, TphA3II. 2 * 48763, sequence analysis, TphA2II + 2 * 17236, sequence analysis, TphA3II
49000
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2 * 49000, alpha, + 2 * 18000, beta, oxygenase component of the TER dioxygenase system, SDS-PAGE
126000
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oxygenase component of the TER dioxygenase system, gel filtration
130000
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gel filtration and native-PAGE, TphA3II
140000
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gel filtration and native-PAGE, TphA2II
235000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
hexamer
monomer
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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about 20% of maximum TPADO activity, whereas activity is completely lost at pH 5
702731
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
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about 60% of maximum TPADO activity, whereas activity is completely lost at 50°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
oxygenase component of the TER dioxygenase system. The reductase component or components cannot be purified
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to near homogeneity by Ni affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) cells
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His-tag fused tphA1II and His-tag fused tphA2IIA3II genes expressed in Escherichia coli BL21(DE3) harboring pET-16tpa1 and pET-16tpa23 respectively, under the control of the T7 promoter
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