Information on EC 1.14.12.14 - 2-aminobenzenesulfonate 2,3-dioxygenase

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The expected taxonomic range for this enzyme is: Alcaligenes

EC NUMBER
COMMENTARY hide
1.14.12.14
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RECOMMENDED NAME
GeneOntology No.
2-aminobenzenesulfonate 2,3-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-aminobenzenesulfonate + NADH + H+ + O2 = 2,3-dihydroxybenzenesulfonate + NH3 + NAD+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
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Microbial metabolism in diverse environments
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orthanilate degradation
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SYSTEMATIC NAME
IUBMB Comments
2-aminobenzenesulfonate,NADH:oxygen oxidoreductase (2,3-hydroxylating, ammonia-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
156621-16-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
Alcaligenes sp. O-1 inducibly deaminates 2-aminobenzenesulfonate via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator, overview. The overall degradative process for ABS in strain O-1 is complex, overview; Alcaligenes sp. O-1 inducibly deaminates 2-aminobenzenesulfonate via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator, overview. The overall degradative process for ABS in strain O-1 is complex, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzenesulfonate + NADH + H+ + O2
2,3-dihydroxybenzenesulfonate + NH3 + NAD+
show the reaction diagram
2-Aminobenzenesulfonate + NADH + H+ + O2
3-Sulfocatechol + NH3 + NAD+
show the reaction diagram
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-
-
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2-aminobenzenesulfonate + NADH + H+ + O2
?
show the reaction diagram
2-aminobenzenesulfonate + NADH + O2
3-sulfocatechol + NH3 + NAD+
show the reaction diagram
4-toluenesulfonate + NADH + O2
?
show the reaction diagram
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-
-
-
?
benzenesulfonate + NADH + O2
?
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-aminobenzenesulfonate + NADH + H+ + O2
2,3-dihydroxybenzenesulfonate + NH3 + NAD+
show the reaction diagram
Q9RBG4, Q9RBG5
2-aminobenzenesulfonate is used in the manufacture of dyes and pharmaceuticals, and, typical of xenobiotic aryl-sulfonates. The compound is degraded in few native environments, e.g. by Alcaligenes sp. strain O-1, the overall degradative process for ABS in strain O-1 is complex, overview. The pathway shows both split operons and dispersed pathway genes, and no transcriptional regulator
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?
2-aminobenzenesulfonate + NADH + H+ + O2
?
show the reaction diagram
2-aminobenzenesulfonate + NADH + O2
3-sulfocatechol + NH3 + NAD+
show the reaction diagram
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enables utilization of 2-aminobenzenesulfonate as sole carbon and energy source
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
part of the reductive component; part of the reductive component
iron-sulfur centre
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one [2Fe-2S] Rieske center per alpha-subunit
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
o-phenanthroline
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029
2-Aminobenzenesulfonate
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0.108
4-toluenesulfonate
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0.018
benzenesulfonate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16000
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alpha-beta, 2 * 46000 + 2 * 16000, SDS-PAGE
46000
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alpha-beta, 2 * 46000 + 2 * 16000, SDS-PAGE
134000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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alpha-beta, 2 * 46000 + 2 * 16000, SDS-PAGE
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to freezing and thawing and exposure to air
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene absAa, DNA and amino acid sequence determination and analysis. Six genes of the abs cluster are co-transcribed and contain the genes, absAa and absAb, for two subunits of the oxygenase component of the ABS-dioxygenase system. Sequence comparisons, overview; gene absAb, DNA and amino acid sequence determination and analysis. Six genes of the abs cluster are co-transcribed and contain the genes, absAa and absAb, for two subunits of the oxygenase component of the ABS-dioxygenase system. Sequence comparisons, overview