Information on EC 1.14.12.1 - anthranilate 1,2-dioxygenase (deaminating, decarboxylating)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.14.12.1
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RECOMMENDED NAME
GeneOntology No.
anthranilate 1,2-dioxygenase (deaminating, decarboxylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
anthranilate + NAD(P)H + 2 H+ + O2 = catechol + CO2 + NAD(P)+ + NH3
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
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decarboxylation
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oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-nitrobenzoate degradation II
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anthranilate degradation I (aerobic)
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indole-3-acetate degradation VIII (bacterial)
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Aminobenzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
anthranilate,NAD(P)H:oxygen oxidoreductase (1,2-hydroxylating, deaminating, decarboxylating)
Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9059-17-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KU-15
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Manually annotated by BRENDA team
strain KU-15
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Manually annotated by BRENDA team
wild-type ATCC 15926, ATCC 29574
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Manually annotated by BRENDA team
No.23 (ATCC 11250)
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Manually annotated by BRENDA team
strain CA10, two component type of enzyme
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Manually annotated by BRENDA team
strain CA10, two component type of enzyme
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxybenzoic acid + NAD(P)H + O2 + H2O
?
show the reaction diagram
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?
anthranilic acid + alanine + O2
?
show the reaction diagram
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
show the reaction diagram
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
show the reaction diagram
anthranilic acid + NADH + O2 + H2O
catechol + CO2 + NAD+ + NH3
show the reaction diagram
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?
benzoate + NAD(P)H + O2 + H2O
benzoate dihydrodiol + ?
show the reaction diagram
catechol + O2 + NAD(P)H + H2O
?
show the reaction diagram
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?
protocatechuic acid + NAD(P)H + O2 + H2O
?
show the reaction diagram
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?
salicylic acid + NAD(P)H + O2 + H2O
?
show the reaction diagram
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?
additional information
?
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salicylic acid and o-aminophenol are no substrates
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
anthranilic acid + alanine + O2
catechol + CO2 + NH3 + ?
show the reaction diagram
anthranilic acid + NAD(P)H + O2 + H2O
catechol + CO2 + NAD(P)+ + NH3
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iron-sulfur centre
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-chloromercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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oxygenase component completely inactive at pH 9
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18900
AndAd, product of ORF4, calculated from deduced amino acid sequence; AndAd, product of ORF4, calculated from deduced amino acid sequence
48000
AndAc, product of ORF3, calculated from deduced amino acid sequence; AndAc, product of ORF3, calculated from deduced amino acid sequence
220000
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gel filtration, wild type and mutant enzymes
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
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apha, beta, 3 * 52000 + 3 * 24000, SDS-PAGE, native mass by gel filtration
trimer
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1 * 19000 + 1 * 39000 + 1 * 54000, DNA-sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein, expressed in Escherichia coli
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wild type and mutant enzymes
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
clustered antABC genes from ADP1 chromosome encodes anthranilate dioxygenase
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clustered antABC genes from ADP1 chromosome encodes anthranilate dioxygenase; expressed in Escherichia coli
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expressed in Escherichia coli TOP10F'; expressed in Escherichia coli TOP10F'
expression of mutant enzymes in Escherichia coli BL21-Codon Plus
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D218A
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no activity
D218E
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no activity
D218N
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no activity
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