Information on EC 1.14.11.B3 - L-proline cis-4-hydroxylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.11.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
L-proline cis-4-hydroxylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-proline + 2-oxoglutarate + O2 = cis-4-hydroxy-L-proline + succinate + CO2
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
L-proline,2-oxoglutarate:oxygen oxidoreductase (4-cis-hydroxylating)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Rhizobium loti, strain MAFF303099, gene MlP4H
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-proline + 2-oxoglutarate + O2
cis-4-hydroxy-L-proline + succinate + CO2
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-proline + 2-oxoglutarate + O2
cis-4-hydroxy-L-proline + succinate + CO2
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
diethyldicarbonate
Fe3+
32% inhibition at 2 mM
hydroxylamine
12% inhibition at 2 mM
Mg2+
26% inhibition at 2 mM
Mn2+
30% inhibition at 2 mM
additional information
no inhibition by hydroxylamine, Mg2+, Mn2+, and Fe3+ at 2 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbic acid
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25 - 0.3
2-oxoglutarate
0.54
L-proline
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24 - 25
2-oxoglutarate
24 - 25
L-proline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.684
purified recombinant enzyme
0.707
purified recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 35
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli by affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene MlP4H, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli JM109
gene SmP4H, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli JM109
in order to develop a microbial production system for N-acetyl CHOP from L-proline, a coexpression plasmid harboring the genes encoding SmP4H and Mpr1 from Sinorhizobium meliloti and Saccharomyces cerevisiae, respectively is constructed, and introduced into Escherichia coli BL21 DELTAputA strain in which the L-proline degradation pathway is blocked
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis