Information on EC 1.14.11.B16 - carotenoid 4,4'-beta-ionone ring oxygenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.14.11.B16
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
carotenoid 4,4'-beta-ionone ring oxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
beta-carotene + 2-oxoglutarate + O2 + H2O = canthaxanthin + succinate + CO2 + 4 H+
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
beta-carotene:oxygen 4,4'-oxidoreductase (canthaxanthin-forming)
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3R,2'S)-myxol 2'-alpha-L-fucoside + acceptor + O2
(3R,2'S)-4-oxomyxol 2'-alpha-L-fucoside + reduced acceptor
show the reaction diagram
3-hydroxyechinenone + acceptor + O2
phoenicoxanthin + reduced acceptor
show the reaction diagram
4-oxozeaxanthin + acceptor + O2
astaxanthin + reduced acceptor
show the reaction diagram
beta-carotene + 2-oxoglutarate + O2
canthaxanthin + ?
show the reaction diagram
beta-carotene + acceptor + O2
canthaxanthin + reduced acceptor
show the reaction diagram
beta-carotene + acceptor + O2
echinenone + reduced acceptor
show the reaction diagram
echinenone + acceptor + O2
canthaxanthin + reduced acceptor
show the reaction diagram
zeaxanthin + 2-oxoglutarate + O2
4-oxozeaxanthin + ?
show the reaction diagram
zeaxanthin + acceptor + O2
adonixanthin + reduced acceptor
show the reaction diagram
zeaxanthin + acceptor + O2
astaxanthin + reduced acceptor
show the reaction diagram
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enzyme CrtW148
via adonixanthin as intermediate
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
ascorbate
catalase
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0143
3-hydroxyechinenone
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30C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.0083
4-oxozeaxanthin
-
30C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.0013
beta-carotene
-
30C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.002
echinenone
-
30C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
0.01
zeaxanthin
-
30C, 0.4 M Tris/HCl, pH 8.0, 1 mM dithiothreitol, 0.5 mM FeSO4, 5 mM ascorbic acid, 0.5 mM 2-oxoglutarate, 0.1% (mass/vol) deoxycholate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000000083
0.00000015
0.00000017
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substrate zeaxanthin, 30C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid
0.0000002
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substrate zeaxanthin, 30C, 0.4 M Tris-HCl, pH 8.0, 1 mM dithiothreitol, 0.1% v/v Tween 60, 3 mM ATP, 0.5 mM FeSO4, 0.5 mM 2-oxoglutarate, 5 mM ascorbic acid
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli