Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2
(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2
(S)-4-hydroxy-L-norvaline + 2-oxoglutarate + O2
?
(S)-allyl-L-cysteine + 2-oxoglutarate + O2
S-allyl-L-cysteine (S)-sulfoxide + succinate + CO2
(S)-ethyl-L-cysteine + 2-oxoglutarate + O2
S-ethyl-L-cysteine (S)-sulfoxide + succinate + CO2
(S)-methyl-L-cysteine + 2-oxoglutarate + O2
S-methyl-L-cysteine (S)-sulfoxide + succinate + CO2
4-hydroxy-L-norleucine + 2-oxoglutarate + O2
?
DL-homocysteine + 2-oxoglutarate + O2
?
-
-
-
-
?
L-allo-isoleucine + 2-oxoglutarate + O2
(S)-3-hydroxy-L-allo-isoleucine + succinate + CO2
-
-
-
-
?
L-cysteine + 2-oxoglutarate + O2
?
-
-
-
-
?
L-ethionine + 2-oxoglutarate + O2
L-ethionine (S)-sulfoxide + succinate + CO2
L-ethionine + 2-oxoglutarate + O2
L-ethionine sulfoxide + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
N,N-dihydroxy-L-isoleucine + succinate + CO2
L-leucine + 2-oxoglutarate + O2
4-hydroxy-L-leucine + succinate + CO2
-
-
-
-
?
L-leucine + 2-oxoglutarate + O2
?
L-methionine + 2-oxoglutarate + O2
?
L-methionine + 2-oxoglutarate + O2
L-methionine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
L-methionine + 2-oxoglutarate + O2
L-methionine sulfoxide + succinate + CO2
-
-
-
-
?
L-norleucine + 2-oxoglutarate + O2
4-hydroxy-L-norleucine + succinate + CO2
-
-
-
-
?
L-norvaline + 2-oxoglutarate + O2
(S)-4-hydroxy-L-norvaline + succinate + CO2
-
-
-
-
?
additional information
?
-
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2
-
-
-
-
?
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2
(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2
-
-
-
-
?
(S)-4-hydroxy-L-norvaline + 2-oxoglutarate + O2

?
-
-
-
-
?
(S)-4-hydroxy-L-norvaline + 2-oxoglutarate + O2
?
-
-
-
-
?
(S)-allyl-L-cysteine + 2-oxoglutarate + O2

S-allyl-L-cysteine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
(S)-allyl-L-cysteine + 2-oxoglutarate + O2
S-allyl-L-cysteine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
(S)-ethyl-L-cysteine + 2-oxoglutarate + O2

S-ethyl-L-cysteine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
(S)-ethyl-L-cysteine + 2-oxoglutarate + O2
S-ethyl-L-cysteine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
(S)-methyl-L-cysteine + 2-oxoglutarate + O2

S-methyl-L-cysteine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
(S)-methyl-L-cysteine + 2-oxoglutarate + O2
S-methyl-L-cysteine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
4-hydroxy-L-norleucine + 2-oxoglutarate + O2

?
-
-
-
-
?
4-hydroxy-L-norleucine + 2-oxoglutarate + O2
?
-
-
-
-
?
L-ethionine + 2-oxoglutarate + O2

L-ethionine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
L-ethionine + 2-oxoglutarate + O2
L-ethionine (S)-sulfoxide + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

(2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
best substrate
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
best substrate
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

(4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

N,N-dihydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
N,N-dihydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-leucine + 2-oxoglutarate + O2

?
-
-
-
?
L-leucine + 2-oxoglutarate + O2
?
-
-
-
?
L-methionine + 2-oxoglutarate + O2

?
-
-
-
?
L-methionine + 2-oxoglutarate + O2
?
-
-
-
?
additional information

?
-
no activity with L-threonine
-
-
-
additional information
?
-
-
the enzyme does not react with D-Ile, D-allo-Ile, tert-butoxycarbonyl-L-Ile, 9-fluorenylmethoxycarbonyl-L-Ile,N-carbamoyl-L-Ile, L-Ile methyl ester, and N-acetyl-L-Ile methyl ester
-
-
-
additional information
?
-
no activity with L-threonine
-
-
-
additional information
?
-
-
the enzyme does not react with D-Ile, D-allo-Ile, tert-butoxycarbonyl-L-Ile, 9-fluorenylmethoxycarbonyl-L-Ile,N-carbamoyl-L-Ile, L-Ile methyl ester, and N-acetyl-L-Ile methyl ester
-
-
-
additional information
?
-
-
L-isoleucine, 2-oxoglutarate: oxygen oxidoreductase HilA and L-isoleucine-4'-dioxygenase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-4' and C-4 positions, respectively, to synthesize L-4',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase
-
-
-
additional information
?
-
-
L-isoleucine, 2-oxoglutarate: oxygen oxidoreductase HilA and L-isoleucine-4'-dioxygenase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-4' and C-4 positions, respectively, to synthesize L-4',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2
(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
L-isoleucine + 2-oxoglutarate + O2
N,N-dihydroxy-L-isoleucine + succinate + CO2
additional information
?
-
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2
-
-
-
-
?
(2S,3R,4S)-4-hydroxy-L-isoleucine + 2-oxoglutarate + O2
(2S,3R)-2-amino-3-methyl-4-ketopentanoate + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

(2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
best substrate
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
best substrate
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(2S,3R,4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

(4S)-4-hydroxy-L-isoleucine + succinate + CO2
E2GIN1
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
(4S)-4-hydroxy-L-isoleucine + succinate + CO2
E2GIN1
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2

N,N-dihydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
L-isoleucine + 2-oxoglutarate + O2
N,N-dihydroxy-L-isoleucine + succinate + CO2
-
-
-
-
?
additional information

?
-
-
L-isoleucine, 2-oxoglutarate: oxygen oxidoreductase HilA and L-isoleucine-4'-dioxygenase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-4' and C-4 positions, respectively, to synthesize L-4',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase
-
-
-
additional information
?
-
-
L-isoleucine, 2-oxoglutarate: oxygen oxidoreductase HilA and L-isoleucine-4'-dioxygenase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-4' and C-4 positions, respectively, to synthesize L-4',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Hibi, M.; Kawashima, T.; Kodera, T.; Smirnov, S.; Sokolov, P.; Sugiyama, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
Characterization of Bacillus thuringiensis L-isoleucine dioxygenase for production of useful amino acids
Appl. Environ. Microbiol.
77
6926-6930
2011
Bacillus thuringiensis, Bacillus thuringiensis 2e2
brenda
Kodera, T.; Smirnov, S.; Samsonova, N.; Kozlov, Y.; Koyama, R.; Hibi, M.; Ogawa, J.; Yokozeki, K.; Shimizu, S.
A novel l-isoleucine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine
Biochem. Biophys. Res. Commun.
390
506-510
2009
Bacillus thuringiensis, Bacillus thuringiensis 2e2 AKU 0251
brenda
Smirnov, S.V.; Sokolov, P.M.; Kodera, T.; Sugiyama, M.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
A novel family of bacterial dioxygenases that catalyse the hydroxylation of free L-amino acids
FEMS Microbiol. Lett.
331
97-104
2012
Bacillus thuringiensis (E2GIN1), Bacillus thuringiensis 2-e-2 (E2GIN1)
brenda
Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis
MicrobiologyOpen
2
471-481
2013
Pantoea ananatis, Pantoea ananatis AJ13355
brenda
Hibi, M.; Kawashima, T.; Yajima, H.; Smirnov, S.; Kodera, T.; Sugiyama, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.
Enzymatic synthesis of chiral amino acid sulfoxides by Fe(II)/alpha-ketoglutarate-dependent dioxygenase
Tetrahedron Asymmetry
24
990-994
2013
Bacillus thuringiensis, Bacillus thuringiensis 2e2
-
brenda