Information on EC 1.14.11.3 - pyrimidine-deoxynucleoside 2'-dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.14.11.3
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RECOMMENDED NAME
GeneOntology No.
pyrimidine-deoxynucleoside 2'-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2'-deoxyuridine + 2-oxoglutarate + O2 = uridine + succinate + CO2
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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oxidative decarboxylation
-
-
-
-
redox reaction
-
-
-
-
reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
2'-deoxyuridine,2-oxoglutarate:oxygen oxidoreductase (2'-hydroxylating)
Requires Fe(II) and ascorbate. Also acts on thymidine. cf. EC 1.14.11.10, pyrimidine-deoxynucleoside 1'-dioxygenase.
CAS REGISTRY NUMBER
COMMENTARY hide
9076-89-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain STA 4
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-
Manually annotated by BRENDA team
line 221a
-
-
Manually annotated by BRENDA team
line 221a
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-bromodeoxyuridine + 2-oxoglutarate + O2
5-bromouridine + succinate + CO2
show the reaction diagram
5-hydroxymethyldeoxyuridine + 2-oxoglutarate + O2
5-hydroxymethyluridine + succinate + CO2
show the reaction diagram
6-azathymidine + 2-oxoglutarate + O2
5-mehyluridine + succinate + CO2
show the reaction diagram
thymidine + 2-oxoglutarate + O2
5-hydroxymethyluridine + succinate + CO2
show the reaction diagram
thymidine + 2-oxoglutarate + O2
5-methyluridine + succinate + CO2
show the reaction diagram
thymidylate + 2-oxoglutarate + O2
5-methyluridine 5'-phosphate + succinate + CO2
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thymidine + 2-oxoglutarate + O2
5-hydroxymethyluridine + succinate + CO2
show the reaction diagram
thymidine + 2-oxoglutarate + O2
5-methyluridine + succinate + CO2
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
at 0.02 mM complete inhibition
2,4-pyridinedicarboxylic acid
2-ketoadipate
-
38% inhibition at 2.5 mM
2-ketopimelate
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26% inhibition at 2.5 mM
3-ketoadipate
-
50% inhibition at 2.5 mM
3-oxoglutarate
-
27% inhibition at 2.5 mM
Co2+
-
95% inhibition at 0.25 mM
Cu2+
-
95% inhibition at 0.25 mM
Diglycolate
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18% inhibition at 2.5 mM
dimethyloxoglycine
glutamate
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20% inhibition at 2.5 mM
iminodiacetate
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28% inhibition at 2.5 mM
Mn2+
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60% inhibition at 0.25 mM
N2
-
complete inhibition by exclusion of oxygen
Ni2+
-
95% inhibition at 0.25 mM
Thiodiglycolate
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24% inhibition at 2.5 mM
Zn2+
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95% inhibition at 0.25 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
catalase
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
2-oxoglutarate
-
-
0.15
5-bromodeoxyuridine
-
-
0.29
5-Hydroxymethyldeoxyuridine
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-
0.29
6-Azathymidine
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-
0.19
deoxyuridine
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-
0.45
Fe2+
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at pH 7.2 in 50 mM phosphate buffer
0.09
thymidine
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-
0.07
thymidylate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.053
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with thymidine as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
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1 * 46000, SDS-PAGE
47000
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gel filtration
64600
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification
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recombinant His-tagged JBP1 from Escherichia coli strain BL21-DE3 T1R by metal affinty chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant expression of His-tagged JBP1 in Escherichia coli strain BL21-DE3 T1R
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information