Information on EC 1.13.11.68 - 9-cis-beta-carotene 9',10'-cleaving dioxygenase

Word Map on EC 1.13.11.68
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.68
-
RECOMMENDED NAME
GeneOntology No.
9-cis-beta-carotene 9',10'-cleaving dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9-cis-beta-carotene + O2 = 9-cis-10'-apo-beta-carotenal + beta-ionone
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
5-deoxystrigol biosynthesis
-
-
Carotenoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
9-cis-beta-carotene:O2 oxidoreductase (9',10'-cleaving)
Requires Fe2+. The enzyme participates in a pathway leading to biosynthesis of strigolactones, plant hormones involved in promotion of symbiotic associations known as arbuscular mycorrhiza.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
tomato plants expressing a SlCCD7 antisense construct display greatly increased branching. A metabolomic screen is conducted. With the exception of a reduction of stem amino acid content in the transgenic lines, no major changes are observed. Targeted analysis of the same plants reveal significantly decreased levels of strigolactone. There are no significant changes in root carotenoids, indicating that relatively little substrate is required to produce the bioactive strigolactones. The germination rate of Orobanche ramosa seeds is reduced by up to 90% on application of extract from the SlCCD7 antisense lines, compared with the wild type. Upon mycorrhizal colonization, C13 cyclohexenone and C14 mycorradicin apocarotenoid levels are greatly reduced in the roots of the antisense lines, implicating SlCCD7 in their biosynthesis
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9-cis-beta-carotene + 02
9-cis-beta-apo-10'-carotenal + beta-ionone
show the reaction diagram
beta-carotene + O2
10'-apo-beta-carotenal + beta-ionone
show the reaction diagram
-
-
-
?
additional information
?
-
no substrate: lycopene, lutein, zeaxanthin, violaxanthin, or neoxanthin
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methanol
-
addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0152
beta-carotene
-
pH 7.0, temperature not specified in the publication
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
protein contains a probable chloroplast targeting sequence of 31 amino acids
Manually annotated by BRENDA team
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Methanol
-
addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as a thioredoxin and GST fusion proteins in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information