Information on EC 1.13.11.36 - chloridazon-catechol dioxygenase

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The expected taxonomic range for this enzyme is: Phenylobacterium

EC NUMBER
COMMENTARY hide
1.13.11.36
-
RECOMMENDED NAME
GeneOntology No.
chloridazon-catechol dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 = 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone 1,2-oxidoreductase (decyclizing)
An iron protein, requiring additional Fe2+. Not identical with EC 1.13.11.1 (catechol 1,2-dioxygenase), EC 1.13.11.2 (catechol 2,3-dioxygenase) or EC 1.13.11.5 (homogentisate 1,2-dioxygenase). Involved in the breakdown of the herbicide chloridazon.
CAS REGISTRY NUMBER
COMMENTARY hide
82869-32-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
various strains
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-methylpyrocatechol + O2
2-hydroxy-3-methylmuconic acid semialdehyde
show the reaction diagram
-
-
-
-
?
4-methylpyrocatechol + O2
2-hydroxy-3-methylmuconic acid semialdehyde
show the reaction diagram
-
-
-
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
show the reaction diagram
catechol + O2
2-hydroxymuconic acid semialdehyde
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can partially replace Fe2+
Zn2+
-
can partially replace Fe2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
High ionic strength
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ascorbate
-
protects against autooxidation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017
3-Methylpyrocatechol
-
-
0.16
4-Methylpyrocatechol
-
-
0.2
5-Amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone
-
-
0.66
pyrocatechol
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.3
catechol
Phenylobacterium immobile
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.15
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
-
6 * 34000, SDS-PAGE
200000
-
analytical ultracentrifugation
220000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
6 * 34000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
with 37% ammonium sulfate
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high osmotic sensitivity
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivated by oxygen, can be reactivated by Fe2+ or ascorbate, Fe2+ much more effective in reactivation
-
439433
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for months
-
4°C, crystal suspensions stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE