Information on EC 1.13.11.28 - 2,3-dihydroxybenzoate 2,3-dioxygenase

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The expected taxonomic range for this enzyme is: Tecoma stans

EC NUMBER
COMMENTARY hide
1.13.11.28
-
RECOMMENDED NAME
GeneOntology No.
2,3-dihydroxybenzoate 2,3-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,3-dihydroxybenzoate + O2 = 2-carboxy-cis,cis-muconate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxybenzoate:oxygen 2,3-oxidoreductase (decyclizing)
Also acts, more slowly, with 2,3-dihydroxy-4-methylbenzoate and 2,3-dihydroxy-4-isopropylbenzoate.
CAS REGISTRY NUMBER
COMMENTARY hide
56802-97-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxy-p-cumate + O2
2-carboxy-4-isopropyl-cis,cis-muconate
show the reaction diagram
-
28% of activity
-
-
?
2,3-dihydroxy-p-toluate + O2
2-carboxy-4-methyl-cis,cis-muconate
show the reaction diagram
-
38% of activity
-
-
?
2,3-dihydroxybenzoate + O2
2-carboxy-cis,cis-muconate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxybenzoate + O2
2-carboxy-cis,cis-muconate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
preincubation with substrate protects against inactivation
8-hydroxyquinoline
-
reversible by addition of Cu2+
arsenite
-
-
Cd2+
-
-
Co2+
-
-
diethyldithiocarbamate
-
reversible by addition of Cu2+
dithiothreitol
-
preincubation with substrate protects against inactivation
glutathione
iodoacetate
-
preincubation with substrate protects against inactivation
L-cysteine
-
preincubation with substrate protects against inactivation
N-ethylmaleimide
Neocuproine
-
reversible by addition of Cu2+
p-chloromercuribenzoate
Salicylaldoxime
-
reversible by addition of Cu2+
Semicarbazide
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
65
2,3-Dihydroxybenzoate
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
no activity above pH 7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
30% of activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-20
-
cannot be stored at -20°C except as ammonium sulfate precipitate or adsorbed to tricalcium phosphate gel
55
-
complete inactivation after 20 min, addition of 0.006 mM substrate protects against inactivation for 15 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
chloroplast enzyme more stable than soluble enzyme
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-