Information on EC 1.13.11.26 - peptide-tryptophan 2,3-dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.26
-
RECOMMENDED NAME
GeneOntology No.
peptide-tryptophan 2,3-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[protein]-L-tryptophan + O2 = [protein]-N-formyl-L-kynurenine
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
[protein]-L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing)
Also acts on tryptophan.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-64-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
field-grown
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Manually annotated by BRENDA team
Willd.
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Manually annotated by BRENDA team
Wistar, male
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-tryptophan + O2
formylkynurenine
show the reaction diagram
-
-
-
-
?
DL-5-fluorotryptophan + O2
5-fluoroformylkynurenine
show the reaction diagram
-
-
-
-
?
DL-5-methyltryptophan + O2
5-methyl-N-formylkynurenine
show the reaction diagram
-
-
-
-
?
DL-6-fluorotryptophan + O2
4-fluoro-N-formylkynurenine
show the reaction diagram
-
-
-
-
?
DL-6-methyltryptophan + O2
4-methyl-N-formylkynurenine
show the reaction diagram
-
-
-
-
?
DL-7-methyltryptophan + O2
3-methyl-N-formylkynurenine
show the reaction diagram
-
-
-
-
?
DL-tryptophan + O2
formylkynurenine
show the reaction diagram
-
-
-
-
?
DL-tryptophan methyl ester + O2
formylkynurenine methyl ester
show the reaction diagram
-
-
-
-
?
ethyl N-acetyl-L-tryptophan + O2
ethyl 2-acetamido-3-2'-formimidobenzoyl propionate
show the reaction diagram
L-5-hydroxytryptophan + O2
5-hydroxy-N-formylkynurenine
show the reaction diagram
-
very poor substrate activity with molecular form A
-
-
?
L-tryptophan + O2
2-oxotryptophan
show the reaction diagram
L-tryptophan + O2
formylkynurenine
show the reaction diagram
L-tryptophan methyl ester + O2
N-formylkynurenine methyl ester
show the reaction diagram
-
-
-
-
?
N-acetyl-5-methyltryptophan + O2
5-methyl-Nalpha-acetyl-Nomega-formylkynurenine
show the reaction diagram
-
-
-
-
?
N-acetyl-DL-tryptophan + O2
N-acetylformylkynurenine
show the reaction diagram
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-
-
-
?
N-acetyltryptophan + O2
Nalpha-acetyl-Nomega-formylkynurenine
show the reaction diagram
tryptophan + O2
L-kynurenine
show the reaction diagram
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-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-tryptophan + O2
formylkynurenine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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at 10 mM strong inhibition
Cd2+
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at 10 mM strong inhibition
Cu2+
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at 10 mM strong inhibition
Sr2+
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at 10 mM strong inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
alpha,alpha'-dipyridyl
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desipramine
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antidepressant, exogenous melatonin counteracts the inhibitory effect
dithioerythritol
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2 mM, total activity loss
dithiothreitol
fluoxetine
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antidepressant, exogenous melatonin counteracts the inhibitory effect
N-bromosuccinimide
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N-ethylmaleimide
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o-phenanthroline
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p-chloromercuribenzoate
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p-hydroxymercurisulfonate
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10 mM, activity loss of 60%
Soybean trypsin inhibitor
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
L-tryptophan
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0.2
N-acetyltryptophan
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
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Tris-HCl buffer
8
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sodium sulfate buffer
8.6
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in Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
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form C with chymotrypsin as substrate
30
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enzyme assay
37 - 45
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form A, tryptophan or gamma-chymotrypsin as substrates
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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embryo, radicle, coleoptile
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
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molecular form A, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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70% activity loss after 20 min preincubation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4C, stable for more than a year, less stable at -15C
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0-4C, stable for six months
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4C, forms A and C stable for at least two weeks, form B activity loss after three days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
three molecular forms: A,B,C
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two forms in the radicle
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