Information on EC 1.13.11.25 - 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.25
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RECOMMENDED NAME
GeneOntology No.
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
androstenedione degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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Steroid degradation
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androgen and estrogen metabolism
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SYSTEMATIC NAME
IUBMB Comments
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen 4,5-oxidoreductase (decyclizing)
Requires Fe2+. Also acts on 3-isopropylcatechol and 3-tert-butyl-5-methylcatechol.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-63-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
show the reaction diagram
3-isopropylcatechol + O2
?
show the reaction diagram
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?
3-methylcatechol
?
show the reaction diagram
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7.4% of activity with 3-isopropylcatechol
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?
3-tert-butyl-5-methylcatechol + O2
?
show the reaction diagram
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?
4-methylcatechol
?
show the reaction diagram
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4.3% of activity with 3-isopropylcatechol
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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1.13 gatom per mol of enzyme, stimulation of reaction
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
4-hydroxymercuribenzoate
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1 mM, 87% inhibition after 10 min
4-Isopropylcatechol
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competitive vs. organic substate, uncompetitive vs. O2
8-hydroxyquinoline
o-phenanthroline
sodium iodoacetate
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1 mM, 70% inhibition after 10 min
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
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inactive below
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
274000
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sedimentation equilibrium
286000
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
acetone stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10% acetone, relatively long perids, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetone, freezing and thawing, DEAE-cellulose, calcium phosphate cellulose
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