Information on EC 1.13.11.23 - 2,3-dihydroxyindole 2,3-dioxygenase

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The expected taxonomic range for this enzyme is: uncultured bacterium

EC NUMBER
COMMENTARY hide
1.13.11.23
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RECOMMENDED NAME
GeneOntology No.
2,3-dihydroxyindole 2,3-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,3-dihydroxyindole + O2 = anthranilate + CO2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Tryptophan metabolism
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SYSTEMATIC NAME
IUBMB Comments
2,3-dihydroxyindole:oxygen 2,3-oxidoreductase (decyclizing)
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CAS REGISTRY NUMBER
COMMENTARY hide
37256-62-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
an aerobic Gram-positive coccus isolated from soil, grown on indole
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxyindole + O2
anthranilate + CO2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxyindole + O2
anthranilate + CO2
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-Dimercaptopropanol
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47% inhibition at 1 mM, 59% inhibition at 5 mM
ascorbic acid
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30% inhibition at 1 mM
catechol
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45% inhibition at 1 mM
o-phenanthroline
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46% inhibition at 1 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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unstable below
439539
7 - 9
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stable
439539
10
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unstable above
439539
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 7, 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using sonic extracts, protamine sulfate treatment, ammonium sulfate fractionation, treatment with alumina C-gamma gel and column chromatography on DEAE-cellulose
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