Information on EC 1.13.11.19 - cysteamine dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.19
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RECOMMENDED NAME
GeneOntology No.
cysteamine dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-aminoethanethiol + O2 = hypotaurine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
taurine biosynthesis
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Taurine and hypotaurine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
2-aminoethanethiol:oxygen oxidoreductase
A non-heme iron protein that is involved in the biosynthesis of taurine. Requires catalytic amounts of a cofactor-like compound, such as sulfur, sufide, selenium or methylene blue for maximal activity. 3-Aminopropanethiol (homocysteamine) and 2-mercaptoethanol can also act as substrates, but glutathione, cysteine, and cysteine ethyl- and methyl esters are not good substrates [1,3].
CAS REGISTRY NUMBER
COMMENTARY hide
9033-41-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-mercaptopropionic acid + O2
3-sulfinopropionic acid
show the reaction diagram
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-
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?
cysteamine + O2
hypotaurine
show the reaction diagram
homocysteamine + O2
2-aminopropansulfinic acid
show the reaction diagram
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-
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?
mercaptoethanol + O2
2-hydroxyethanesulfinic acid
show the reaction diagram
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-
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?
N-acetylcysteamine + O2
N-acetylhypotaurine
show the reaction diagram
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-
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?
pantetheine + O2
pantothenate + cysteamine
show the reaction diagram
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oxidized at less than 3% of the cysteamine-dependent rate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cysteamine + O2
hypotaurine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu
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1 atom of Cu per molecule of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,3-dithiopropane
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1,4-dithiobutane
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2-mercaptoethanol
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inhibition at high concentration, activation at low concentration
8-hydroxyquinoline
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complete inhibition at 7.5 mM
alpha,alpha'-dipyridyl
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slight inhibition
cystamine
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cysteamine
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cysteine
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diethyldithiocarbamate
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70% inhibition at 10 mM
KCN
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60-70% inhibition at 10 mM
mercaptoethylguanidine
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noncompetitive to cysteamine
Neocuproine
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45-55% inhibition at 10 mM
o-phenanthroline
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45-55% inhibition at 10 mM
pantetheine
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S
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
Salicylaldoxime
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slight inhibition
Se
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
Sulfide
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sulfide, elemental sulfur, elemental selenium or hydroxylamine required in catalytic amount, inhibition when added over a critical concentration (with the exception of hydroxylamine)
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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stimulation at low concentration, inhibition at high concentration
hydroxylamine
methylene blue
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cofactor-like compound
Sulfide
sulfur
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
3-Mercaptopropionic acid
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0.001 - 0.62
cysteamine
0.83
homocysteamine
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1.2
mercaptoethanol
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0.71
N-acetylcysteamine
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0.1
O2
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
1,3-dithiopropane
0.007
mercaptoethylguanidine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000021
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substrate: hypertaurine, kidney, activity is lower than in mice kidney
0.000026
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substrate: hypertaurine, liver, activity is lower than in mice liver
0.000037
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substrate: hypertaurine, kidney, higher activity in mice kidney than in rat kidney
0.000062
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substrate: hypertaurine, brain
0.000091
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substrate: hypertaurine, liver, higher activity in mice liver than in rat liver
2.8
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3 - 3.5
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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7.8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Cysteamine levels in plasma of rats fed the cysteamine supplemented diet are significantly higher than those in tissues of rats fed basal diet. Rats fed the cysteamine-supplemented diet have no markedly elevated levels of hypotaurine in plasma.
Manually annotated by BRENDA team
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high activity
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
83000
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sedimentation-diffusion equilibrium method
92000
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sedimentation equilibrium
96000
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gel filtration
100000
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method not mentioned
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
exhaustive dialysis against water: partial denaturation, dialysis against water brought to pH 7.5 with concentrated ammonia or against 0.01 M potassium phosphate buffer, pH 7.6, has no effect
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, up to 3 years
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0°C, 70% saturated ammonium sulfate, stable for months
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0°C, dialyzed and diluted solution, loss of 20% activity per week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpressed in HepG2/C3A cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H95A
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catalytically inactive
additional information
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Vanin 1-/- and +/+ mice