Information on EC 1.13.11.17 - indole 2,3-dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.17
-
RECOMMENDED NAME
GeneOntology No.
indole 2,3-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
indole + O2 = 2-formylaminobenzaldehyde
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
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-
-
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reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indole degradation to anthranil and anthranilate
-
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Tryptophan metabolism
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SYSTEMATIC NAME
IUBMB Comments
indole:oxygen 2,3-oxidoreductase (decyclizing)
Enzymes from the plants Tecoma stans, Jasminum grandiflorum and Zea mays are flavoproteins containing copper. They are part of enzyme systems that form either anthranil (2,1-benzoisoxazole) (Tecoma stans), anthranilate (Jasminum grandiflorum) or both (Zea mays) as the final product. A second enzyme from Tecoma stans is not a flavoprotein, does not require copper, and is part of a system that forms anthranilate as the final product.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-57-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-bromoindole + O2
5-bromo-2-formylaminobenzaldehyde
show the reaction diagram
5-hydroxyindole + O2
2-formylamino-5-hydroxybenzaldehyde
show the reaction diagram
5-methylindole + O2
2-formylamino-5-methylbenzaldehyde
show the reaction diagram
indole + O2
?
show the reaction diagram
-
during daytime activity is less, it reaches a peak during nighttime
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-
-
indole + O2
anthranilic acid
show the reaction diagram
o-aminobenzaldehyde + O2
anthranilic acid
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
indole + O2
?
show the reaction diagram
-
during daytime activity is less, it reaches a peak during nighttime
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
8-hydroxyquinoline
Atebrin
azide
CO
-
0.5 mM, 58% inhibition
Cu2+
-
1 mM, CuSO4, 25% inhibition
cyanide
diethyldithiocarbamate
Dithionite
dithiothreitol
-
0.0005 mM, 97% inhibition
iodoacetate
-
0.0005 mM, 19% inhibition
Neocuproine
-
0.001 mM, 92% inhibition
Salicylaldoxime
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0189 - 0.14
indole
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0157
-
disappearance of 1 nmol indole in 1 min
0.0441
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disappearance of 1 nmol indole in 1 min
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8 - 6.2
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pH 3.8: about 80% of maximal activity, pH 5.8: about 65% of maximal activity, pH 6.2: about 30% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
activity is present in relevant amounts even in newborn rats. The specific activity increases significantly in young rats by about 44% in comparison to newborns
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
-
gel filtration
98000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 6
-
stable
395575
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis results in complete loss of activity
dialysis, 8 h, results in complete loss of activity
-
pH 7, 0.02 M sodium phosphate buffer, 0.1 mM EDTA, 6 h in the cold, slight loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 18 h without appreciable loss of activity, complete loss of activity after 48 h
-
-20°C, 20 d, no appreciable loss of activity
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4°C, stable for 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE