Reference on EC 1.12.1.2 - hydrogen dehydrogenase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Burgdorf, T.; De Lacey, A.L.; Friedrich, B.
Functional analysis by site-directed mutagenesis of the NAD+-reducing hydrogenase from Ralstonia eutropha
J. Bacteriol.
184
6280-6288
2002
Cupriavidus necator
Tran-Betcke, A.; Warnecke, U.; Bcker, C.; Zaborosch, C.; Friedrich, B.
Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16
J. Bacteriol.
172
2920-2929
1990
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Pfitzner, J.; Linke, H.A.B.; Schlegel, H.G.
Properties of the NAD-specific hydrogenase from Hydrogenomonas H 16
Arch. Mikrobiol.
71
67-78
1970
Hydrogenomonas sp., Hydrogenomonas sp. H16
Rohde, M.; Johannssen, W.; Mayer, F.
Immunocytochemical localization of the soluble NAD-dependent hydrogenase in cells of alcaligenes eutrophus
FEMS Microbiol. Lett.
36
83-86
1986
Cupriavidus necator
-
Hyman, M.R.; Arp, D.J.
Reversible and irreversible effects of nitric oxide on the soluble hydrogenase from Alcaligenes eutrophus H16
Biochem. J.
254
469-475
1988
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Hyman, M.R.; Fox, C.A.; Arp, D.J.
Role of hydrogen in the activation and regulation of hydrogen oxidation by the soluble hydrogenase from Alcaligenes eutrophus H16
Biochem. J.
254
463-468
1988
Cupriavidus necator
Popov, V.O.; Ovchinnikov, A.N.; Utkin, I.B.; Gazaryan, I.G.; Egorov, A.M.; Berezin, I.V.
Inactivation of the NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophus Z1: Thermoinactivation mechanism
Biochim. Biophys. Acta
831
297-301
1985
Cupriavidus necator, Cupriavidus necator Z1
-
Popov, V.O.; Gazaryan, I.G.; Egorov, A.M.; Berezin, I.V.
NAD-dependent hydrogenase from the hydrogen-oxidizing bacterium Alcaligenes eutrophum Z1. Kinetic studies of the NADH-dehydrogenase activity
Biochim. Biophys. Acta
827
466-471
1985
Cupriavidus necator, Cupriavidus necator Z1
-
Schneider, K.; Schlegel, H.G.
Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16
Biochim. Biophys. Acta
452
66-80
1976
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Schneider, K.; Schlegel, H.G.
Localization and stability of hydrogenases from aerobic hydrogen bacteria
Arch. Microbiol.
112
229-238
1977
Cupriavidus necator, no activity in Alcaligenes paradoxus, no activity in Aquaspirillum autotrophicum, no activity in Corynebacterium autotrophicum, no activity in Paracoccus denitrificans, no activity in Pseudomonas facilis, no activity in Hydrogenophaga palleronii, Cupriavidus necator G27, Cupriavidus necator N9A, Cupriavidus necator b19, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Aggag, M.; Schlegel, H.G.
Studies on a gram-positive hydrogen bacterium, Nocardia opaca 1 b. III. Purification, stability and some properties of the soluble hydrogen dehydrogenase
Arch. Microbiol.
100
25-39
1974
Rhodococcus opacus, Rhodococcus opacus 1B
Schneider, K.; Schlegel, H.G.; Jochim, K.
Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1 b
Eur. J. Biochem.
138
553-541
1984
Rhodococcus opacus, Rhodococcus opacus 1B
-
Schneider, K.; Cammack, R.; Schlegel, H.G.
Content and localization of FMN, Fe-S clusters and nickel in the NAD-linked hydrogenase of Nocardia opaca 1b
Eur. J. Biochem.
142
75-84
1984
Rhodococcus opacus, Rhodococcus opacus 1B
Petrov, R.R.; Utkin, I.B.; Popov, V.O.
Redox-dependent inactivation of the NAD-dependent hydrogenase from Alcaligenes eutrophus Z1
Arch. Biochem. Biophys.
268
298-305
1989
Cupriavidus necator, Cupriavidus necator Z1
Petrov, R.R.; Utkin, I.B.; Popov, V.O.
Effect of redox potential on the activation of the NAD-dependent hydrogenase from Alcaligenes eutrophus Z1
Arch. Biochem. Biophys.
268
287-297
1989
Cupriavidus necator, Cupriavidus necator Z1
Friedrich, C.G.; Suetin, S.; Lohmeyer, M.
Nickel and iron incoorporation into soluble hydrogenase of alcaligenes eutrophus
Arch. Microbiol.
140
206-211
1984
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
Friedrich, C.G.; Schneider, K.; Friedrich, B.
Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus
J. Bacteriol.
152
42-48
1982
Cupriavidus necator
Schneider, K.; Schlegel, H.G.
Production of superoxide radicals by soluble hydrogenase from Alcaligenes eutrophus H16
Biochem. J.
193
99-107
1981
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Schneider, K.; Cammack, R.; Schlegel, H.G.; Hall, D.O.
The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus
Biochim. Biophys. Acta
578
445-461
1979
Cupriavidus necator
Schneider, K.; Schlegel, H.G.
Identification and quantitative determination of the flavin component of soluble hydrogenase from Alcaligenes eutrophus
Biochem. Biophys. Res. Commun.
84
564-571
1978
Cupriavidus necator
Grzeszik, C.; Ross, K.; Schneider, K.; Reh, M.; Schlegel, H.G.
Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22
Arch. Microbiol.
167
172-176
1997
Achromobacter denitrificans, Achromobacter denitrificans 4a-2, Achromobacter ruhlandii, Cupriavidus necator, Cupriavidus necator Cd2/01, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Rhodococcus opacus
Happe, R.P.; Roseboom, W.; Egert, G.; Friedrich, C.G.; Massanz, C.; Friedrich, B.; Albracht, S.P.
Unusual FTIR and EPR properties of the H2-activating site of the cytoplasmic NAD-reducing hydrogenase from Ralstonia eutropha
FEBS Lett.
466
259-263
2000
Cupriavidus necator
Porthun, A.; Bernhard, M.; Friedrich, B.
Expression of a functional NAD-reducing [NiFe] hydrogenase from the gram-positive Rhodococcus opacus in the gram-negative Ralstonia eutropha
Arch. Microbiol.
177
159-166
2002
Rhodococcus opacus, Rhodococcus opacus MR11
Rakhely, G.; Kovacs, A.T.; Maroti, G.; Fodor, B.D.; Csanadi, G.; Latinovics, D.; Kovacs, K.L.
Cyanobacterial-type, heteropentameric, NAD+-reducing NiFe hydrogenase in the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina
Appl. Environ. Microbiol.
70
722-728
2004
Thiocapsa roseopersicina (Q6XQK5), Thiocapsa roseopersicina
Hanczar, T.; Csaki, R.; Bodrossy, L.; Murrell, J.C.; Kovacs, K.L.
Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism
Arch. Microbiol.
177
167-172
2002
Methylococcus capsulatus
Loescher, S.; Burgdorf, T.; Buhrke, T.; Friedrich, B.; Dau, H.; Haumann, M.
Non-standard structures of the Ni-Fe cofactor in the regulatory and the NAD-reducing hydrogenases from Ralstonia eutropha
Biochem. Soc. Trans.
33
25-27
2005
Cupriavidus necator
Tikhonova, T.V.; Savel'eva, N.D.; Popov, V.O.
Chemical modification of catalytically essential functional groups of NAD-dependent hydrogenase from Ralstonia eutropha H16
Biochemistry
68
994-1001
2003
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Schmitz, O.; Boison, G.; Salzmann, H.; Bothe, H.; Schutz, K.; Wang, S.H.; Happe, T.
HoxE - a subunit specific for the pentameric bidirectional hydrogenase complex (HoxEFUYH) of cyanobacteria
Biochim. Biophys. Acta
1554
66-74
2002
Synechocystis sp. (Q9Z354)
Leul, M.; Mohapatra, A.; Sellstedt, A.
Biodiversity of hydrogenases in Frankia
Curr. Microbiol.
50
17-23
2005
Frankia sp.
Mohapatra, A.; Leul, M.; Mattsson, U.; Sellstedt, A.
A hydrogen-evolving enzyme is present in Frankia sp. R43
FEMS Microbiol. Lett.
236
235-240
2004
Frankia sp. R43 (P22317), Frankia sp. R43 (P22318), Frankia sp. R43 (P22319), Frankia sp. R43 (P22320)
Burgdorf, T.; van der Linden, E.; Bernhard, M.; Yin, Q.Y.; Back, J.W.; Hartog, A.F.; Muijsers, A.O.; de Koster, C.G.; Albracht, S.P.; Friedrich, B.
The soluble NAD+-Reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 consists of six subunits and can be specifically activated by NADPH
J. Bacteriol.
187
3122-3132
2005
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Van der Linden, E.; Burgdorf, T.; Bernhard, M.; Bleijlevens, B.; Friedrich, B.; Albracht, S.P.
The soluble [NiFe]-hydrogenase from Ralstonia eutropha contains four cyanides in its active site, one of which is responsible for the insensitivity towards oxygen
J. Biol. Inorg. Chem.
9
616-626
2004
Cupriavidus necator
Soboh, B.; Linder, D.; Hedderich, R.
A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis
Microbiology
150
2451-2463
2004
Caldanaerobacter subterraneus subsp. tengcongensis
Loescher, S.; Burgdorf, T.; Zebger, I.; Hildebrandt, P.; Dau, H.; Friedrich, B.; Haumann, M.
Bias from H2 cleavage to production and coordination changes at the Ni-Fe active site in the NAD+-reducing hydrogenase from Ralstonia eutropha
Biochemistry
45
11658-11665
2006
Cupriavidus necator
Tikhonova, T.V.; Kurkin, S.A.; Klyachko, N.L.; Popov, V.O.
Use of a reverse micelle system for study of oligomeric structure of NAD+-reducing hydrogenase from Ralstonia eutropha H16
Biochemistry
70
645-651
2005
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Serebryakova, L.T.; Sheremetieva, M.E.
Characterization of catalytic properties of hydrogenase isolated from the unicellular cyanobacterium Gloeocapsa alpicola CALU 743
Biochemistry
71
1370-1376
2006
Gloeocapsa alpicola, Gloeocapsa alpicola CALU 743
Vignais, P.M.
H/D exchange reactions and mechanistic aspects of the hydrogenases
Coord. Chem. Rev.
249
1677-1690
2005
Cupriavidus necator
-
Burgdorf, T.; Loescher, S.; Liebisch, P.; Van der Linden, E.; Galander, M.; Lendzian, F.; Meyer-Klaucke, W.; Albracht, S.P.; Friedrich, B.; Dau, H.; Haumann, M.
Structural and oxidation-state changes at its nonstandard Ni-Fe site during activation of the NAD-reducing hydrogenase from Ralstonia eutropha detected by X-ray absorption, EPR, and FTIR spectroscopy
J. Am. Chem. Soc.
127
576-592
2005
Cupriavidus necator
van der Linden, E.; Burgdorf, T.; de Lacey, A.L.; Buhrke, T.; Scholte, M.; Fernandez, V.M.; Friedrich, B.; Albracht, S.P.
An improved purification procedure for the soluble [NiFe]-hydrogenase of Ralstonia eutropha: new insights into its (in)stability and spectroscopic properties
J. Biol. Inorg. Chem.
11
247-260
2006
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Burgdorf, T.; Lenz, O.; Buhrke, T.; van der Linden, E.; Jones, A.K.; Albracht, S.P.; Friedrich, B.
[NiFe]-hydrogenases of Ralstonia eutropha H16: modular enzymes for oxygen-tolerant biological hydrogen oxidation
J. Mol. Microbiol. Biotechnol.
10
181-196
2005
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Arai, T.; Watanabe, S.; Matsumi, R.; Atomi, H.; Imanaka, T.; Miki, K.
Crystallization and preliminary X-ray crystallographic study of [NiFe]-hydrogenase maturation factor HypE from Thermococcus kodakaraensis KOD1
Acta Crystallogr. Sect. F
63
765-767
2007
Thermococcus kodakarensis
Kellers, P.; Ogata, H.; Lubitz, W.
Purification, crystallization and preliminary X-ray analysis of the membrane-bound [NiFe] hydrogenase from Allochromatium vinosum
Acta Crystallogr. Sect. F
64
719-722
2008
Allochromatium vinosum
Rakhely, G.; Laurinavichene, T.V.; Tsygankov, A.A.; Kovacs, K.L.
The role of Hox hydrogenase in the H2 metabolism of Thiocapsa roseopersicina
Biochim. Biophys. Acta
1767
671-676
2007
Thiocapsa roseopersicina, Thiocapsa roseopersicina Bbs
Oliveira, P.; Lindblad, P.
An AbrB-like protein regulates the expression of the bidirectional hydrogenase in Synechocystis sp. strain PCC 6803
J. Bacteriol.
190
1011-1019
2008
Synechocystis sp.
Rangarajan, E.S.; Asinas, A.; Proteau, A.; Munger, C.; Baardsnes, J.; Iannuzzi, P.; Matte, A.; Cygler, M.
Structure of [NiFe] hydrogenase maturation protein HypE from Escherichia coli and its interaction with HypF
J. Bacteriol.
190
1447-1458
2008
Escherichia coli
Watanabe, S.; Matsumi, R.; Arai, T.; Atomi, H.; Imanaka, T.; Miki, K.
Crystal structures of [NiFe] hydrogenase maturation proteins HypC, HypD, and HypE: insights into cyanation reaction by thiol redox signaling
Mol. Cell
27
29-40
2007
Thermococcus kodakarensis
Fdez Galvan, I.; Volbeda, A.; Fontecilla-Camps, J.C.; Field, M.J.
A QM/MM study of proton transport pathways in a [NiFe] hydrogenase
Proteins
73
195-203
2008
Solidesulfovibrio fructosivorans
Germer, F.; Zebger, I.; Saggu, M.; Lendzian, F.; Schulz, R.; Appel, J.
Overexpression, isolation and spectroscopic characterization of the bidirectional [NiFe]-hydrogenase from Synechocystis sp. PCC 6803
J. Biol. Chem.
284
36462-36472
2009
Synechocystis sp.
Kiss, E.; Kos, P.B.; Vass, I.
Transcriptional regulation of the bidirectional hydrogenase in the cyanobacterium Synechocystis 6803
J. Biotechnol.
142
31-37
2009
Synechocystis sp. PCC 6803
Ohki, Y.; Yasumura, K.; Kuge, K.; Tanino, S.; Ando, M.; Li, Z.; Tatsumi, K.
Thiolate-bridged dinuclear iron(tris-carbonyl)-nickel complexes relevant to the active site of [NiFe] hydrogenase
Proc. Natl. Acad. Sci. USA
105
7652-7657
2008
synthetic construct
Schut, G.J.; Adams, M.W.
The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production
J. Bacteriol.
191
4451-4457
2009
Thermotoga maritima
Worm, P.; Stams, A.J.; Cheng, X.; Plugge, C.M.
Growth- and substrate-dependent transcription of formate dehydrogenase and hydrogenase coding genes in Syntrophobacter fumaroxidans and Methanospirillum hungatei
Microbiology
157
280-289
2011
Syntrophobacter fumaroxidans, Methanospirillum hungatei, Methanospirillum hungatei JF-1 (DSM 864), Syntrophobacter fumaroxidans MPOB (DSM 10017)
Ratzka, J.; Lauterbach, L.; Lenz, O.; Ansorge-Schumacher, M.
Systematic evaluation of the dihydrogen-oxidising and NAD +-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 as a cofactor regeneration catalyst
Biocatal. Biotransform.
29
246-252
2011
Cupriavidus necator, Cupriavidus necator DSM 428
-
Lauterbach, L.; Liu, J.; Horch, M.; Hummel, P.; Schwarze, A.; Haumann, M.; Vincent, K.; Lenz, O.; Zebger, I.
The hydrogenase subcomplex of the NAD+-reducing [NiFe] hydrogenase from Ralstonia eutropha - Insights into catalysis and redox interconversions
Eur. J. Inorg. Chem.
2011
1067-1079
2011
Cupriavidus necator (P22319), Cupriavidus necator (P22320), Cupriavidus necator DSM 428 (P22319), Cupriavidus necator DSM 428 (P22320)
-
Herr, N.; Ratzka, J.; Lauterbach, L.; Lenz, O.; Ansorge-Schumacher, M.
Stability enhancement of an O2-tolerant NAD+-reducing [NiFe]-hydrogenase by a combination of immobilisation and chemical modification
J. Mol. Catal. B
97
169-174
2013
Cupriavidus necator, Cupriavidus necator DSM 428
-
Lauterbach, L.; Idris, Z.; Vincent, K.; Lenz, O.
Catalytic properties of the isolated diaphorase fragment of the NAD +-reducing [NiFe]-hydrogenase from Ralstonia eutropha
PLoS ONE
6
e25939
2011
Cupriavidus necator (P22317), Cupriavidus necator (P22318), Cupriavidus necator, Cupriavidus necator DSM 428 (P22317), Cupriavidus necator DSM 428 (P22318)
Schiffels, J.; Pinkenburg, O.; Schelden, M.; Aboulnaga, e.l.-.H.A.; Baumann, M.E.; Selmer, T.
An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from Cupriavidus necator in Escherichia coli
PLoS ONE
8
e68812
2013
Cupriavidus necator
Burroughs, N.J.; Boehm, M.; Eckert, C.; Mastroianni, G.; Spence, E.M.; Yu, J.; Nixon, P.J.; Appel, J.; Mullineaux, C.W.; Bryan, S.J.
Solar powered biohydrogen production requires specific localization of the hydrogenase
Energy Environ. Sci.
7
3791-3800
2014
Synechocystis sp. PCC 6803
Puggioni, V.; Tempel, S.; Latifi, A.
Distribution of hydrogenases in Cyanobacteria a phylum-wide genomic survey
Front. Genet.
7
223
2016
Cupriavidus necator (P22319), Cupriavidus necator ATCC 17699 (P22319)
Cano, M.; Volbeda, A.; Guedeney, G.; Aubert-Jousset, E.; Richaud, P.; Peltier, G.; Cournac, L.
Improved oxygen tolerance of the Synechocystis sp. PCC 6803 bidirectional hydrogenase by site-directed mutagenesis of putative residues of the gas diffusion channel
Int. J. Hydrogen Energy
39
16872-16884
2014
Synechocystis sp.
-
Gutekunst, K.; Chen, X.; Schreiber, K.; Kaspar, U.; Makam, S.; Appel, J.
The bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803 is reduced by flavodoxin and ferredoxin and is essential under mixotrophic, nitrate-limiting conditions
J. Biol. Chem.
289
1930-1937
2014
Synechocystis sp. PCC 6803
De Rosa, E.; Checchetto, V.; Franchin, C.; Bergantino, E.; Berto, P.; Szabo, I.; Giacometti, G.M.; Arrigoni, G.; Costantini, P.
[NiFe]-hydrogenase is essential for cyanobacterium Synechocystis sp. PCC 6803 aerobic growth in the dark
Sci. Rep.
5
12424
2015
Synechocystis sp. PCC 6803
Zorin, N.; Medina, M.; Pusheva, M.; Gogotov, I.; Cammack, R.
Hydrogenase from the thermophilic bacterium Thermococcus stetteri Isolation and characterisation of EPR-detectable redox centres
FEMS Microbiol. Lett.
142
71-76
1996
Thermococcus stetteri, Thermococcus stetteri K-15
-
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