Information on EC 1.11.1.B8 - bromide peroxidase (metal-containing)

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.11.1.B8
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
bromide peroxidase (metal-containing)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
RH + HBr + H2O2 = RBr + 2 H2O
show the reaction diagram
Brings about the bromination of a range of organic molecules, forming stable C-Br bonds. Contains Fe(III), Zn(II), Ni(II), Co(II), but neither Vanadium nor heme
-
-
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SYSTEMATIC NAME
IUBMB Comments
bromide:hydrogen-peroxide oxidoreductase (metal-containing)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
IF-3 strain
-
-
Manually annotated by BRENDA team
IF-3 strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
?
show the reaction diagram
I- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
?
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
contains no heme
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
contains 0.35 mol of cobalt ions per mol of enzyme. Addition of 0.1 mM CoCl2 results in 2.1fold increase in activity
Fe3+
-
contains 2.0 mol of ferric iron per mol of enzyme
Ni2+
-
contains 1.0 mol of nickel ions per mol of enzyme
Zn2+
-
contains 0.8 mol of zinc ions per mol of enzyme
additional information
-
contains no vanadium ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
0.1 mM, 97% inhibition
dithioerythritol
-
-
FeCl3
-
0.1 mM, 45% inhibition
hydrazine
-
-
hydroxylamine
-
-
KCN
-
0.1 mM, 82% inhibition
monoiodoamine
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.3
H2O2
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
2 * 33000
68000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.4
-
2 h, 25°C, complete loss of activity below pH 3.5
672311
4 - 11
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2 h, 25°C, stable
672311
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 20
-
pH 7.0, 20 min, stable
30 - 35
-
pH 7.0, 20 min, 10% loss of activity
40
-
pH 7.0, 20 min, 20% loss of activity
50 - 60
-
pH 7.0, 20 min, 10% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE