Information on EC 1.11.1.21 - catalase-peroxidase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.11.1.21
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RECOMMENDED NAME
GeneOntology No.
catalase-peroxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 H2O2 = O2 + 2 H2O
show the reaction diagram
donor + H2O2 = oxidized donor + 2 H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methanol oxidation to formaldehyde IV
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reactive oxygen species degradation
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superoxide radicals degradation
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methane metabolism
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Phenylalanine metabolism
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Tryptophan metabolism
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Phenylpropanoid biosynthesis
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Drug metabolism - other enzymes
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
donor:hydrogen-peroxide oxidoreductase
Differs from EC 1.11.1.7, peroxidase in having a relatively high catalase (EC 1.11.1.6) activity with H2O2 as donor, releasing O2; both activities use the same heme active site. In Mycobacterium tuberculosis it is responsible for activation of the commonly used antitubercular drug, isoniazid.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
formerly Acinetobacter sp.
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Manually annotated by BRENDA team
formerly Acinetobacter sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
formerly Synechococcus sp. strain PCC 6301
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
gene katG, KatG is a bifunctional enzyme showing both catalase and peroxidase activities
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 H2O2
2 H2O + O2
show the reaction diagram
2 H2O2
O2 + 2 H2O
show the reaction diagram
2 H2O2
O2 + H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
? + H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + tert-butyl hydroperoxide
oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + ?
show the reaction diagram
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?
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
?
show the reaction diagram
2,4-dichlorophenol + H2O2
?
show the reaction diagram
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?
2,6-dimethoxyphenol + H2O2
oxidized 2,6-dimethoxyphenol + H2O
show the reaction diagram
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?
2-dianisidine + tert-butyl hydroperoxide
?
show the reaction diagram
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peroxidase activity
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?
3,3',5,5'-tetramethylbenzidine + H2O2
? + H2O
show the reaction diagram
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?
3,3',5,5'-tetramethylbenzidine + H2O2
oxidized 3,3',5,5'-tetramethylbenzidine + H2O
show the reaction diagram
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-
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?
3,3'-diaminobenzidine + H2O2
oxidized 3,3'-diaminobenzidine + H2O
show the reaction diagram
3,3'-dimethoxybenzidine + H2O2
oxidized 3,3'-dimethoxybenzidine + H2O
show the reaction diagram
3-chloro-peroxybenzoic acid
?
show the reaction diagram
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?
3-chloroperoxybenzoic acid
?
show the reaction diagram
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?
3-methoxyphenol + H2O2
? + H2O
show the reaction diagram
4-aminoantipyrine + H2O2
oxidized 4-aminoantipyrine + H2O
show the reaction diagram
4-phenylenediamine + H2O2
oxidized 4-phenylenediamine + H2O
show the reaction diagram
aminoantipyrine + H2O2
oxidized aminoantipyrine + H2O
show the reaction diagram
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?
aniline + H2O2
? + H2O
show the reaction diagram
ascorbate + H2O2
monodehydroascorbate + H2O
show the reaction diagram
catechol + H2O2
? + H2O
show the reaction diagram
chlorpromazine + H2O2
? + H2O
show the reaction diagram
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?
diaminobenzidine + H2O2
oxidized diaminobenzidine + H2O
show the reaction diagram
ethanol + H2O2
acetaldehyde + ?
show the reaction diagram
guaiacol + H2O2
?
show the reaction diagram
guaiacol + H2O2
oxidized guaiacol + H2O
show the reaction diagram
guajacol + H2O2
? + H2O
show the reaction diagram
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peroxidase activity
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?
H2O2
O2 + H2O
show the reaction diagram
H2O2 + o-dianisidine
?
show the reaction diagram
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peroxidase activity
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?
isonazid + H2O2
?
show the reaction diagram
isonazid + H2O2
oxidized isonazid + H2O
show the reaction diagram
isoniazid + H2O2
?
show the reaction diagram
isoniazid + H2O2
oxidized isoniazid + H2O
show the reaction diagram
isoniazid + NADH
isonicotinic acyl-NADH
show the reaction diagram
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isoniazid activation
the product is a strong inhibitor of the NADH-dependent enoyl-[acyl carrier protein] reductase InhA and the beta-ketoacyl [acyl carrier protein] synthase
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?
isoniazid + phenol + H2O2
benzoquinone + ?
show the reaction diagram
methanol + H2O2
formaldehyde + H2O
show the reaction diagram
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at pH 4.5
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?
N,N,N',N'-tetramethyl-p-phenylenediamine + H2O2
? + H2O
show the reaction diagram
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?
NADH + H2O2
?
show the reaction diagram
NADPH + H2O2
?
show the reaction diagram
o-dianisidine + H2O2
?
show the reaction diagram
o-dianisidine + H2O2
? + H2O
show the reaction diagram
o-dianisidine + H2O2
oxidized o-dianisidine + H2O
show the reaction diagram
o-dianisidine + tert-butyl hydroperoxide
o-dianisidine quinonediimine + ?
show the reaction diagram
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?
o-dianisidine + tert-butyl hydroperoxide
oxidized o-dianisidine + ?
show the reaction diagram
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?
o-dianisidine + tert-butyl hydroperoxide + H2O2
o-dianisidin quinone diimine + ?
show the reaction diagram
o-phenylenediamine + H2O2
oxidized o-phenylenediamine + H2O
show the reaction diagram
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?
penicillin G + H2O2
penicillin G (R)-sulfoxide + H2O
show the reaction diagram
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?
peroxyacetic acid
?
show the reaction diagram
peroxyacetic acid + 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
?
show the reaction diagram
phenol + H2O2
? + H2O
show the reaction diagram
pyrogallol + H2O2
?
show the reaction diagram
pyrogallol + H2O2
? + H2O
show the reaction diagram
reduced 2,3',6'-trichloroindophenol + H2O2
oxidized 2,3',6'-trichloroindophenol + H2O
show the reaction diagram
reduced cytochrome c + H2O2
oxidized cytochrome c + H2O
show the reaction diagram
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?
tert-butyl hydroperoxide + H2O2
oxidized tert-butyl hydroperoxide + H2O
show the reaction diagram
tert-butyl peroxide + H2O2
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 H2O2
O2 + H2O
show the reaction diagram
H2O2
O2 + H2O
show the reaction diagram
isonazid + H2O2
?
show the reaction diagram
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activation of antituberculosis drug isonazid, catalytic mechanism
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CO
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complexed with the enzyme, structure determination, binding kinetics
Fe
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dioxygen binding to ferrous KatG and Y249F is reversible and monophasic. Ferrous wild-type KatG is rapidly converted by hydrogen peroxide in a two-phasic reaction via compound II to compound III, the latter being also efficiently transformed to ferric KatG. Determination of bimolecular rate constant and dissociation constant
K+
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2 mM K+ have a stimulatory effect on catalase activity
Mg2+
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2 mM Mg2+ have a stimulatory effect on catalase activity
NO
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complexed with the enzyme, structure determination, binding kinetics
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
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acts as an inhibitor of peroxidase activity at lower pH (pH 3.8-4.5)
3-Amino-1,2,4-triazole
3-Amino-4-hydroxybenzoic acid
50 mM, 30% residual activity
Ba2+
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partial inhibition of catalase activity
Cu2+
1 mM, 30% residual activity
cyanide
histidine
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19% inhibition at 10 mM
hydroxylamine
hydroxylamonium chloride
0.5 mM, 70% inhibition
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Isoniazid
Isonicotinic acid hydrazide
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nicotinic acid hydrazide
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strong competitive inhibition
Sodium azide
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25 mM, 30% inhibition of peroxidase activity, 100% inhibition of catalyse activity
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-1,2,4-triazole
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156% relative catalase activity at 10 mM, 121% relative peroxidase activity at 10 mM
ammonium sulfate
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additions of ammonium sulfate even at weak concentrations (10 to 70 mM) stimulate catalatic activity measured in the presence of 1.5 M NaC1 by about 30%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055 - 6
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
0.063 - 0.096
2-dianisidine
0.7
3,3',5,5'-tetramethylbenzidine
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pH 5.0, 23C
0.13
ascorbate
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pH 5.0, 23C
1.1
chlorpromazine
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pH 5.0, 23C
0.06 - 225
H2O2
5.6 - 10.18
isonazid
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0.08 - 0.43
Isoniazid
1.2
N,N,N',N'-tetramethyl-p-phenylenediamine
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pH 5.0, 23C
0.23
NADH
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peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25C
0.17
NADPH
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peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25C
0.04 - 0.49
o-Dianisidine
0.97
peroxyacetic acid
pH 4.5, 37C
0.2 - 1.6
pyrogallol
5.7 - 97.9
tert-butyl hydroperoxide
additional information
additional information
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steady- and transient-state kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.34 - 210000
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
0.86 - 4.85
2-dianisidine
5220
3,3',5,5'-tetramethylbenzidine
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pH 5.0, 23C
520
ascorbate
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pH 5.0, 23C
7940
chlorpromazine
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pH 5.0, 23C
0.0061 - 339000
H2O2
0.057 - 0.081
Isoniazid
7012
N,N,N',N'-tetramethyl-p-phenylenediamine
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pH 5.0, 23C
1.2 - 5600
o-Dianisidine
19.42
peroxyacetic acid
pH 4.5, 37C
27 - 630
pyrogallol