Information on EC 1.1.99.7 - lactate-malate transhydrogenase

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The expected taxonomic range for this enzyme is: Veillonella parvula

EC NUMBER
COMMENTARY hide
1.1.99.7
-
RECOMMENDED NAME
GeneOntology No.
lactate-malate transhydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-lactate + oxaloacetate = pyruvate + malate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Pyruvate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-lactate:oxaloacetate oxidoreductase
Catalyses hydrogen transfer from C3 or C4 (S)-2-hydroxy acids to 2-oxo acids. It contains tightly bound nicotinamide nucleotide in its active centre. This prosthetic group cannot be removed without denaturation of the protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9077-15-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Escherichia coli
-
-
-
Manually annotated by BRENDA team
no activity in Propionibacterium shermanii
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-lactate + oxaloacetate
malate + pyruvate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-lactate + oxaloacetate
malate + pyruvate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ion required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
oxaloacetate
-
-
p-hydroxymercuribenzoate
-
-
additional information
-
non-specific inhibition by high ionic strength
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
L-lactate
-
-
1.4
L-malate
-
-
0.05
oxaloacetate
-
-
2.4
pyruvate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 9.5
-
about 50% of maximal activity at pH 6.3 and 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
assay at
25
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
3 (or 4) * 30000, sucrose gradient centrifugation of succinylated and urea solubilized enzyme
99000 - 100000
-
sucrose density gradient studies
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 30000-43000, SDS-PAGE
trimer or tetramer
-
3 (or 4) * 30000, sucrose gradient centrifugation of succinylated and urea solubilized enzyme
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, as ammonium sulfate suspension, about 10-20 mg of protein per ml, 40% loss of activity after 1 year
-
-20°C, pH 7.4
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-cellulose, DEAE-Sephadex
-