Information on EC 1.1.99.38 - 2-deoxy-scyllo-inosamine dehydrogenase (AdoMet-dependent)

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The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY hide
1.1.99.38
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RECOMMENDED NAME
GeneOntology No.
2-deoxy-scyllo-inosamine dehydrogenase (AdoMet-dependent)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine = 3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
paromamine biosynthesis II
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
2-deoxy-scyllo-inosamine:S-adenosyl-L-methionine 1-oxidoreductase
Involved in the biosynthetic pathway of the aminoglycoside antibiotics of the butirosin family. The enzyme from Bacillus circulans was shown to be a radical S-adenosyl-L-methionine (SAM) enzyme. cf. EC 1.1.1.329, 2-deoxy-scyllo-inosamine dehydrogenase.
CAS REGISTRY NUMBER
COMMENTARY hide
99332-63-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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gene disruption causes the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine and 2-deoxystreptamine. In vitro assay of the overexpressed enzyme reveals that the enzyme catalyzes the oxidation of 2-deoxy-scyllo-inosamine under strictly anaerobic conditions along with consumption of an equimolar amount of S-adenosyl-L-methionine to produce 5-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scyllo-inosose
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine
3-amino-2,3-dideoxy-scyllo-inosose + 5'-deoxyadenosine + L-methionine
show the reaction diagram
2-deoxystreptamine + S-adenosyl-L-methionine
? + 5'-deoxyadenosine + L-methionine
show the reaction diagram
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14.4% of the rate with 2-deoxy-scyllo-inosamine
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?
myo-inositol + S-adenosyl-L-methionine
? + 5'-deoxyadenosine + L-methionine
show the reaction diagram
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0.9% of the rate with 2-deoxy-scyllo-inosamine
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?
scyllo-inositol + S-adenosyl-L-methionine
? + 5'-deoxyadenosine + L-methionine
show the reaction diagram
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3.3% of the rate with 2-deoxy-scyllo-inosamine
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?
additional information
?
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no substrates: 1D-chiro-inositol, 1L-chiro-inositol, muco-inositol, allo-inositol, D-glucose, D-glucosamine, D-xylose
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe-S center
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxy-scyllo-inosamine
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substrate inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.022
2-deoxy-scyllo-inosamine
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pH 8.0, 28C
0.31 - 0.46
S-adenosyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
2-deoxy-scyllo-inosamine
Bacillus circulans
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pH 8.0, 28C
0.02 - 0.021
S-adenosyl-L-methionine
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
analysis of electron paramagnetic resonance signals for free enzyme, the enzyme-S-adenosyl-L-methionine complex, and a rhombic signal presumably derived from the enzyme-S-adenosyl-L-methionine-substrate ternary complex show close interaction of both S-adenosyl-L-methione and 2-deoxy-scyllo-inosamine with the [4Fe-4S] cluster
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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