Information on EC 1.1.99.32 - L-sorbose 1-dehydrogenase

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The expected taxonomic range for this enzyme is: Gluconobacter oxydans

EC NUMBER
COMMENTARY hide
1.1.99.32
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RECOMMENDED NAME
GeneOntology No.
L-sorbose 1-dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-sorbose + acceptor = 1-dehydro-L-sorbose + reduced acceptor
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-keto-L-gulonate biosynthesis
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L-ascorbate biosynthesis III
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ascorbate metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-sorbose:acceptor 1-oxidoreductase
The product, L-sorbosone, is an intermediate in bacterial 2-keto-L-gulonic-acid formation. The activity of this membrane-bound enzyme is stimulated by Fe(III) or Co2+ but is inhibited by Cu2+. The enzyme is highly specific for L-sorbose as other sugars, such as glucose, mannitol and sorbitol, are not substrates. Phenazine methosulfate and DCIP can act as artificial acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
133249-51-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain UV10, enzyme activity is induced by growth on L-sorbose or D-sorbitol as carbon source
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-sorbose + 2,6-dichloroindophenol
L-sorbosone + reduced 2,6-dichloroindophenol
show the reaction diagram
L-sorbose + acceptor
L-sorbosone + reduced acceptor
show the reaction diagram
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-
-
?
L-sorbose + phenazine methosulfate
L-sorbosone + reduced phenazine methosulfate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
0.8 mM, 18% increase in activity
Fe3+
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0.4 mM, 21% increase in activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
0.8 mM, complete loss of activity
EDTA
-
5.2 mM, 13% inhibition
iodoacetate
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4.2 mM, 34% inhibition
Quinine
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4.2 mM, 30% inhibition
sodium fluoroacetate
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9.4 mM, 26% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-sorbose dehydrogenase accelerative protein (SAP)
isolated from Bacillus megaterium
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Triton X-100
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solubilization with Triton X-100 gives 10fold increase in activity compared to solubilization with Tween 80, differential solubilization with Tween 80 + Triton X-100 gives 2fold higher activity than Triton X-100 alone
Tween 80
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solubilization with Triton X-100 gives 10fold increase in activity compared to solubilization with Tween 80, differential solubilization with Tween 80 + Triton X-100 gives 2fold higher activity than Triton X-100 alone
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
100
L-sorbose
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electron acceptor 2,6-dichloroindophenol, pH 7.0, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.897
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pH 7.0, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
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4 * 58000, SDS-PAGE
210000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
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unstable below
677427
6.5 - 8
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677427
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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stable up to
46
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5 min, 50% loss of activity
65
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5 min, 90% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
rather stable in buffer containing 200 mM sorbose, pH 7.0, below 30°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification from membrane fraction
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