Information on EC 1.1.99.29 - pyranose dehydrogenase (acceptor)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.99.29
-
RECOMMENDED NAME
GeneOntology No.
pyranose dehydrogenase (acceptor)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a pyranose + acceptor = a pyranos-2-ulose (or a pyranos-3-ulose or a pyranos-2,3-diulose) + reduced acceptor
show the reaction diagram
-
-
-
-
a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
pyranose:acceptor oxidoreductase
Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. Sugars are oxidized in their pyranose but not in their furanose form.
CAS REGISTRY NUMBER
COMMENTARY hide
190606-21-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
show the reaction diagram
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
show the reaction diagram
2-dehydrolactose + acceptor
2,3-didehydrolactose + reduced acceptor
show the reaction diagram
-
-
-
-
?
2-deoxy-D-galactose + acceptor
?
show the reaction diagram
2-deoxy-D-glucose + 1,4-benzoquinone
?
show the reaction diagram
-
49% of the activity with D-glucose
-
-
?
2-deoxy-D-glucose + acceptor
?
show the reaction diagram
2-deoxy-D-glucose + ferricenium ion
?
show the reaction diagram
-
21% of the activity with D-glucose
-
-
?
2-hydroxymethylphenyl-beta-D-glucopyranoside + acceptor
?
show the reaction diagram
salicin, relative activity towards D-glucose (100%): 78%
-
-
?
3-dehydro-D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
-
-
-
-
?
3-fluoro-3-deoxy-D-glucose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
4-hydroxyphenyl-beta-D-glucopyranoside + acceptor
?
show the reaction diagram
arbutin
-
-
?
6-deoxy-D-glucose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 105%
-
-
?
a pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
-
-
-
?
a pyranoside + acceptor
a 3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
show the reaction diagram
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
show the reaction diagram
aldopyranose + 1,4-benzoquinone
2-aldoketose + hydroquinone
show the reaction diagram
-
more active as electron acceptor than 2,6-dichlorophenolindophenol or ferricyanide, less active than 3,5-di-tert-butyl-1,2-benzoquinone, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
-
-
?
aldopyranose + 2,6-dichlorophenolindophenol
2-aldoketose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
65.9% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
-
-
?
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
show the reaction diagram
aldopyranose + ferricyanide
2-aldoketose + ferrocyanide
show the reaction diagram
-
10.4% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
-
-
?
aldopyranose + quinone
2-aldoketose + hydroquinone
show the reaction diagram
-
C-2 specific sugar oxidoreductase, highly nonspecific, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
-
-
?
alpha,alpha-trehalose + 1,4-benzoquinone
3,3'-didehydro-alpha,alpha-trehalose + hydroquinone
show the reaction diagram
alpha,alpha-trehalose + acceptor
3,3'-didehydro-alpha,alpha-trehalose + reduced acceptor
show the reaction diagram
relative activity towards D-glucose (100%): 27%
-
-
?
beta-lactose + acceptor
?
show the reaction diagram
-
-
-
-
?
beta-lactose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
cellobiitol + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 77%
-
-
?
cellobiose + 1,4-benzoquinone
?
show the reaction diagram
cellobiose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 70%
-
-
?
cellobiose + Fe3+
?
show the reaction diagram
-
-
-
-
?
cellobiose + ferricenium ion
?
show the reaction diagram
-
7% of the activity with D-glucose
-
-
?
cellobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
cellotetraose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 14%
-
-
?
cellotriose + 1,4-benzoquinone
?
show the reaction diagram
-
56% of the activity with D-glucose
-
-
?
D-allose + 1,4-benzoquinone
2-dehydro-D-allose + hydroquinone
show the reaction diagram
-
30% of the activity with D-glucose
-
-
?
D-allose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 15%
-
-
?
D-arabino-2-hexosulose + 1,4-benzoquinone
?
show the reaction diagram
-
59% of the activity with D-glucose
-
-
?
D-arabinose + 1,4-benzoquinone
2-dehydro-D-arabinose + hydroquinone
show the reaction diagram
-
18% of the activity with D-glucose
-
-
?
D-arabinose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-cellobiose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-fructose + acceptor
?
show the reaction diagram
-
-
-
?
D-fructose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-fructose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
show the reaction diagram
D-galactose + 1,4-benzoquinone
?
show the reaction diagram
-
66% of the activity with D-glucose
-
-
?
D-galactose + acceptor
2-dehydro-D-galactose + reduced acceptor
show the reaction diagram
relative activity towards D-glucase (100%): 99%
-
-
?
D-galactose + acceptor
?
show the reaction diagram
D-galactose + benzoquinone
2,3-didehydro-D-galactose + hydroquinone
show the reaction diagram
-
-
two main reaction products
-
?
D-galactose + benzoquinone
2-dehydro-D-galactose + hydroquinone
show the reaction diagram
D-galactose + Fe3+
2-dehydro-D-galactose + Fe2+ + H+
show the reaction diagram
-
-
-
-
?
D-galactose + ferricenium ion
?
show the reaction diagram
-
60% of the activity with D-glucose
-
-
?
D-galactose + ferricenium ion
? + ferrocene
show the reaction diagram
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
show the reaction diagram
-
-
-
-
?
D-glucono-1,5-lactone + 1,4-benzoquinone
?
show the reaction diagram
D-glucono-1,5-lactone + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 42%
-
-
?
D-glucono-1,5-lactone + ferricenium ion
?
show the reaction diagram
-
8% of the activity with D-glucose
-
-
?
D-glucosamine + acceptor
?
show the reaction diagram
-
-
-
-
?
D-glucose +
3-dehydro-D-glucose + Fe2+ + H+
show the reaction diagram
-
pH 8.5
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-
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
show the reaction diagram
-
-
D-arabino-2-hexosulose, 2-ketoglucose
-
?
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
?
show the reaction diagram
D-glucose + 2 1,4-benzoquinone
2,3-didehydro-D-glucose + 2 hydroquinone
show the reaction diagram
-
-
-
-
?
D-glucose + 2,5-chloro-1,4-benzoquinone
?
show the reaction diagram
-
pH 5
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
D-glucose + 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
show the reaction diagram
D-glucose + 3,5-di-t-butyl-1,2-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone
?
show the reaction diagram
-
pH 6
-
-
?
D-glucose + 3-ethylbenzthiazolin-6-sulfonic acid cation radical
?
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
2,3-didehydro-D-glucose + reduced acceptor
show the reaction diagram
-
dioxidation at C2 and C3
-
-
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
show the reaction diagram
-
the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack
-
-
?
D-glucose + acceptor
3-dehydro-D-glucose + reduced acceptor
show the reaction diagram
D-glucose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 100%
-
-
?
D-glucose + benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + CuSO4
?
show the reaction diagram
-
-
-
-
?
D-glucose + dimethylaminomethyl-ferricenium
?
show the reaction diagram
-
-
-
-
?
D-glucose + Fe3+
3-dehydro-D-glucose + Fe2+ + H+
show the reaction diagram
-
-
-
-
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
D-glucose + ferricyanide
?
show the reaction diagram
-
-
-
-
?
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
-
-
-
-
?
D-glucose + methyl-1,4-benzoquinone
?
show the reaction diagram
D-glucose + tetrachloro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + tetrafluoro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-gulose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 7%
-
-
?
D-lyxose + 1,4-benzoquinone
?
show the reaction diagram
-
5% of the activity with D-glucose
-
-
?
D-lyxose + ferricenium ion
?
show the reaction diagram
-
5% of the activity with D-glucose
-
-
?
D-maltoheptaose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-maltopentaose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-maltose + acceptor
?
show the reaction diagram
D-maltose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-maltose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-maltose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-maltotriose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-maltotriose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-maltotriose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-mannose + 1,4-benzoquinone
?
show the reaction diagram
-
32% of the activity with D-glucose
-
-
?
D-mannose + acceptor
?
show the reaction diagram
D-mannose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-mannose + ferricenium ion
?
show the reaction diagram
-
26% of the activity with D-glucose
-
-
?
D-mannose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-ribose + 1,4-benzoquinone
2-dehydro-D-ribose + hydroquinone
show the reaction diagram
-
34% of the activity with D-glucose
-
-
?
D-ribose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 19%
-
-
?
D-ribose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-ribose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-ribulose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 17%
-
-
?
D-sorbose + acceptor
?
show the reaction diagram
-
-
-
?
D-tagatose + acceptor
?
show the reaction diagram
relative activity towards D-glucoase (100%): 67%
-
-
?
D-talose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 10%
-
-
?
D-talose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-talose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-trehalose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
show the reaction diagram
D-xylose + 1,4-benzoquinone
?
show the reaction diagram
-
72% of the activity with D-glucose
-
-
?
D-xylose + 2 1,4-benzoquinone
2,3-didehydro-D-xylose + 2 hydroquinone
show the reaction diagram
-
-
-
?
D-xylose + acceptor
?
show the reaction diagram
D-xylose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-xylose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-xylose + ferricenium ion
?
show the reaction diagram
-
81% of the activity with D-glucose
-
-
?
D-xylose + ferricenium ion
? + ferrocene
show the reaction diagram
D-xylose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
erlose + 1,4-benzoquinone
3-dehydroerlose + hydroquinone
show the reaction diagram
gentiobiose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
L-arabinose + 1,4-benzoquinone
2-dehydro-L-arabinose + hydroquinone
show the reaction diagram
-
113% of the activity with D-glucose
-
-
?
L-arabinose + 1,4-benzoquinone
?
show the reaction diagram
-
54% of the activity with D-glucose
-
-
?
L-arabinose + acceptor
?
show the reaction diagram
L-arabinose + Fe3+
?
show the reaction diagram
-
-
-
-
?
L-arabinose + ferricenium ion
?
show the reaction diagram
-
72% of the activity with D-glucose
-
-
?
L-arabinose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-arabinose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-fucose + acceptor
?
show the reaction diagram
L-glucose + 1,4-benzoquinone
2-dehydro-L-glucose + hydroquinone
show the reaction diagram
-
61% of the activity with D-glucose
-
-
?
L-glucose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 42%
-
-
?
L-glucose + ferricenium ion
?
show the reaction diagram
-
11% of the activity with D-glucose
-
-
?
L-sorbose + 1,4-benzoquinone
2-dehydro-L-sorbose + hydroquinone
show the reaction diagram
-
10% of the activity with D-glucose
-
-
?
L-sorbose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 5%
-
-
?
L-sorbose + Fe3+
?
show the reaction diagram
-
-
-
-
?
L-sorbose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-xylobiose + Fe3+
?
show the reaction diagram
-
-
-
-
?
lactose + 1,4-benzoquinone
?
show the reaction diagram
-
21% of the activity with D-glucose
-
-
?
lactose + acceptor
2-dehydrolactose + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 15%
-
-
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
show the reaction diagram
lactose + acceptor
lactobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + Fe3+
?
show the reaction diagram
-
-
-
-
?
lactose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
lactose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
maltose + 1,4-benzoquinone
?
show the reaction diagram
maltose + ferricenium ion
?
show the reaction diagram
-
45% of the activity with D-glucose
-
-
?
maltotetraose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 9%
-
-
?
maltotriose + 1,4-benzoquinone
?
show the reaction diagram
maltotriose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 12%
-
-
?
maltotriose + ferricenium ion
?
show the reaction diagram
-
14% of the activity with D-glucose
-
-
?
maltotriose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
melezitose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 13%
-
-
?
melibiose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 48%
-
-
?
melizitose + 1,4-benzoquinone
3-dehydromelizitose + hydroquinone
show the reaction diagram
methyl alpha-D-glucopyranoside + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 107%
-
-
?
methyl alpha-D-mannopyranoside + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 5%
-
-
?
methyl beta-D-glucopyranoside + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 58%
-
-
?
methyl-alpha-D-galactopyranose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 17%
-
-
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
?
show the reaction diagram
-
11% of the activity with D-glucose
-
-
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone
show the reaction diagram
methyl-alpha-D-galactopyranoside + ferricenium ion
?
show the reaction diagram
-
8% of the activity with D-glucose
-
-
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
?
show the reaction diagram
-
141% of the activity with D-glucose
-
-
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone
show the reaction diagram
methyl-alpha-D-glucopyranoside + ferricenium ion
?
show the reaction diagram
-
143% of the activity with D-glucose
-
-
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
?
show the reaction diagram
-
30% of the activity with D-glucose
-
-
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone
show the reaction diagram
methyl-beta-D-glucopyranoside + ferricenium ion
?
show the reaction diagram
-
17% of the activity with D-glucose
-
-
?
palatinose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 89%
-
-
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
show the reaction diagram
pyranose + acceptor
2-dehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
3-dehydropyranose + reduced acceptor
show the reaction diagram
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
sucrose + 1,4-benzoquinone
3'-dehydrosucrose + hydroquinone
show the reaction diagram
-
quinone-dependent PDH oxidizes sucrose exclusively at C-3 of its terminal glucopyranosyl moiety, 11% of the activity with D-glucose
oxidation of sucrose at C-3 of its terminal glucopyranosyl moiety
-
?
sucrose + 1,4-benzoquinone
?
show the reaction diagram
sucrose + acceptor
3'-dehydrosucrose + reduced acceptor
show the reaction diagram
relative activity towards D-glucose (100%): 46%
-
-
?
sucrose + acceptor
?
show the reaction diagram
-
-
-
-
?
sucrose + ferricenium ion
?
show the reaction diagram
-
6% of the activity with D-glucose
-
-
?
trehalose + 1,4-benzoquinone
?
show the reaction diagram
trehalose + ferricenium ion
?
show the reaction diagram
-
8% of the activity with D-glucose
-
-
?
xylobiose + acceptor
?
show the reaction diagram
-
-
-
?
xylobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
show the reaction diagram
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
show the reaction diagram
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
show the reaction diagram
-
-
-
-
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
show the reaction diagram
cellobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-fructose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-galactose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
show the reaction diagram
-
-
-
-
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
2,3-didehydro-D-glucose + reduced acceptor
show the reaction diagram
-
dioxidation at C2 and C3
-
-
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
show the reaction diagram
-
the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack
-
-
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
-
-
-
-
?
D-maltose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-mannose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-ribose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-talose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
show the reaction diagram
D-xylose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-xylose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-arabinose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-arabinose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-sorbose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
show the reaction diagram
lactose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
lactose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
maltotriose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
2-dehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
3-dehydropyranose + reduced acceptor
show the reaction diagram
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
xylobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ag+
-
37% inhibition of activity
Al3+
-
no activity
Ca2+
-
no activity
Cu2+
-
no activity
Fe2+
-
no activity
Fe3+
-
39% inhibition of activity
Hg2+
-
25% inhibition of activity
Mg2+
-
no activity
Mn2+
-
no activity
Ni2+
-
no activity
Zn2+
-
no activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
10 mM, 100% inhibition
alpha,alpha-dipyridyl
-
no activity
Cu2+
-
10 mM, 90% inhibition
EDTA
-
no activity
F-
-
no activity
HgCl2
-
10 mM, 100% inhibition
MoO42-
-
no activity
NaN3
-
no activity
NH4+
-
at 2 M in 20 mM sodium phosphate buffer pH 7.0
p-hydroxymercuribenzoate
-
no activity
Phenanthroline
-
no activity
additional information
-
not inhibited by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Mn2+, NH4+, Zn2+, F-, EDTA, NaN3, dithiothreitol, phenanthroline and H2O2, each at 10 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
50 mM: activates, 100 mM: 100% increase in activity at pH 4
NH4+
-
at 0.1-1 M in 20 mM sodium phosphate buffer pH 7.0, not due to increased ionic strenth
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.03 - 1.82
1,4-benzoquinone
0.92
2,5-chloro-1,4-benzoquinone
-
at 30C with 50 mM glucose as susbtrate
0.05
2,6-dichloroindophenol
-
at 30C with 25 mM glucose as substrate
0.18
2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
0.55 - 1.6
2-chloro-1,4-benzoquinone
12.14
2-deoxy-D-galactose
-
0.1 M ethanolamine buffer at pH 9.4
0.65
2-deoxy-D-glucose
-
0.1 M ethanolamine buffer at pH 9.4
0.12
2-methylcyclohexa-2,5-diene-1,4-dione
-
at 30C with 25 mM glucose as susbtrate
0.02
3,5-di-t-butyl-1,2-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
0.22
3,5-di-tert-butyl-1,4-benzoquinone
-
at 30C with 50 mM glucose as susbtrate
0.0017
3-ethylbenzthiazolin-6-sulfonic acid cation radical
-
at 30C with 25 mM glucose as substrate
10.84 - 120
beta-lactose
6.82
cellobiose
0.95
CuSO4
-
at 30C with 25 mM glucose as susbtrate
17
D-arabinose
-
at 30C with 0.4 mM ferricenium as electron acceptor
1.05
D-cellobiose
-
0.1 M ethanolamine buffer at pH 9.4
153
D-fructose
1.02 - 29.8
D-galactose
0.74
D-glucosamine
-
0.1 M ethanolamine buffer at pH 9.4
0.44 - 1.82
D-glucose
0.51
D-maltoheptaose
-
0.1 M ethanolamine buffer at pH 9.4
0.64
D-maltopentaose
-
0.1 M ethanolamine buffer at pH 9.4
0.34 - 12
D-maltose
0.64 - 100
D-maltotriose
0.62 - 108
D-mannose
59.7
D-ribose
137
D-sorbose
ferricenium ion as electron acceptor; ferricenium ion as electron acceptor; ferricenium ion as electron acceptor
79.1
D-talose
0.05
D-trehalose
-
0.1 M ethanolamine buffer at pH 9.4
10.8
D-xylobiose
-
Fe3+ as electron acceptor
1.03 - 29.2
D-xylose
0.42
dimethylaminomethyl-ferricenium
-
at 30C with 25 mM glucose as susbtrate
0.13
Fe3+
-
at 30C with 50 mM glucose as susbtrate
0.12
ferricenium hexafluorophosphate
-
at 30C with 25 mM glucose as susbtrate
0.13 - 0.22
ferricenium ion
0.33
ferricyanide
-
at 30C with 25 mM glucose as susbtrate
0.13 - 0.16
ferrocenium ion
17
gentiobiose
-
at 30C with 0.4 mM ferricenium as electron acceptor
0.44 - 24
L-arabinose
12.31
L-fucose
-
0.1 M ethanolamine buffer at pH 9.4
137
L-sorbose
113.9 - 134
lactose
156
maltotriose
0.18
methyl-1,4-benzoquinone
-
at 30C with 50 mM glucose as susbtrate
0.35
Sucrose
-
0.1 M ethanolamine buffer at pH 9.4
0.28
tetrachloro-1,4-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
0.39
tetrafluoro-1,4-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
10.8
xylobiose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
59 - 76
1,4-benzoquinone
12.9 - 15.1
2-chloro-1,4-benzoquinone
38.4
cellobiose
16.5
D-fructose
46.6 - 48.5
D-galactose
37.8 - 48.06
D-glucose
43.2
D-maltose
29.3
D-mannose
31.4
D-ribose
15.7
D-sorbose
Leucoagaricus meleagris
Q0R4L2, Q3L243
ferricenium ion as electron acceptor; ferricenium ion as electron acceptor; ferricenium ion as electron acceptor
21.3
D-talose
41.5 - 62.4
D-xylose
104 - 203
ferricenium ion
104 - 130
ferrocenium ion
34.7 - 45.7
L-arabinose
15.7
L-sorbose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
31.9 - 41
lactose
23.6
maltotriose
50.4
xylobiose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
54.4 - 57.5
1,4-benzoquinone
315
20.9 - 25.5
2-chloro-1,4-benzoquinone
4395
5.6
cellobiose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
82
0.11
D-fructose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
117
44.2 - 46.2
D-galactose
71
54.8 - 57.5
D-glucose
35
5
D-maltose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
1168
0.27
D-mannose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
216
0.52
D-ribose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
292
0.27
D-talose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
2017
22.9 - 40.3
D-xylose
115
802 - 923
ferricenium ion
998
802 - 812
ferrocenium ion
5441
61.8 - 70.8
L-arabinose
206
0.11
L-sorbose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
685
0.28 - 0.32
lactose
114
0.15
maltotriose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
188
4.7
xylobiose
Leucoagaricus meleagris
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
560
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11.5
-
culture supernatant, at pH 8.5 and 30C
14.1
substrate: D-sorbose, ferricenium ion as electron acceptor; substrate: D-sorbose, ferricenium ion as electron acceptor; substrate: D-sorbose, ferricenium ion as electron acceptor
14.7
-
pH 4.5, 25C, D-glucose and 1,4-benzoquinone as substrates
14.9
substrate: D-fructose, ferricenium ion as electron acceptor; substrate: D-fructose, ferricenium ion as electron acceptor; substrate: D-fructose, ferricenium ion as electron acceptor
16.4
-
pH 6.5, 25C
19.2
substrate: D-talose, ferricenium ion as electron acceptor; substrate: D-talose, ferricenium ion as electron acceptor; substrate: D-talose, ferricenium ion as electron acceptor
21.3
substrate: maltotriose, ferricenium ion as electron acceptor; substrate: maltotriose, ferricenium ion as electron acceptor; substrate: maltotriose, ferricenium ion as electron acceptor
21.9
-
after 1.9fold purification, at pH 8.5 and 30C
26.4
substrate: D-mannose, ferricenium ion as electron acceptor; substrate: D-mannose, ferricenium ion as electron acceptor; substrate: D-mannose, ferricenium ion as electron acceptor
28.2
substrate: D-ribose, ferricenium ion as electron acceptor; substrate: D-ribose, ferricenium ion as electron acceptor; substrate: D-ribose, ferricenium ion as electron acceptor
33.5
substrate: L-arabinose, ferricenium ion as electron acceptor; substrate: L-arabinose, ferricenium ion as electron acceptor; substrate: L-arabinose, ferricenium ion as electron acceptor
34.5
substrate: cellobiose ferricenium ion as electron acceptor; substrate: cellobiose ferricenium ion as electron acceptor; substrate: cellobiose ferricenium ion as electron acceptor
35.6
substrate: lactose, ferricenium ion as electron acceptor; substrate: lactose, ferricenium ion as electron acceptor; substrate: lactose, ferricenium ion as electron acceptor
38.9
substrate: D-maltose, ferricenium ion as electron acceptor; substrate: D-maltose, ferricenium ion as electron acceptor; substrate: D-maltose, ferricenium ion as electron acceptor
39.1
substrate: D-xylose, ferricenium ion as electron acceptor; substrate: D-xylose, ferricenium ion as electron acceptor; substrate: D-xylose, ferricenium ion as electron acceptor
41.4
substrate: D-glucose, ferricenium ion as electron acceptor; substrate: D-glucose, ferricenium ion as electron acceptor; substrate: D-glucose, ferricenium ion as electron acceptor
43.7
substrate: D-galactose, ferricenium ion as electron acceptor; substrate: D-galactose, ferricenium ion as electron acceptor; substrate: D-galactose, ferricenium ion as electron acceptor
45.4
substrate: xylobiose, ferricenium ion as electron acceptor; substrate: xylobiose, ferricenium ion as electron acceptor; substrate: xylobiose, ferricenium ion as electron acceptor
67
-
25C, D-glucose and ferricenium ion as substrates
additional information
-
the recombinant enzyme from Aspergillus nidulans crude extract shows a specific activity of 13.5 units/mg using D-glucose as substrate, at 30C in phosphate buffer, pH 7.5. The recombinant 4.1fold purified enzyme from Aspergillus nidulans shows a specific activity of 55.9 units/mg using D-glucose as substrate, at 30C in phosphate buffer, pH 7.5
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5
-
for glucose and 1,4-benzoquinone as substrates
3.5
-
50 mM sodium citrate buffer, 1,4-benzoquinone as electron acceptor
4
-
acetate buffer, two pH-optima
5
-
using 2-chloro-1,4-benzoquinone as electron acceptor
5.5
-
assay at
6.7
-
assay at
8
-
for glucose and 1,4-benzoquinone as substrates
9.5
-
50 mM glycine buffer, ferricenium ion as electron acceptor
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 10
3 - 9.5
-
activity in the range is higher than 50% of the rate at the acidic optimum at pH 4
7 - 10.5
-
depending on the buffer, highest biosensor response in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min
7 - 8
-
in PBS, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min
8 - 10
-
in 0.1 M ethanolamine buffer, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 mL/min
8 - 9
-
in Tris, applied potential + 300 mV vs. Ag-AgCl, 1 mM glucose, flow rate 1.0 ml/min
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
-
ferricenium ion as electron acceptor
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2
-
isoelectric focusing, five isoforms
4.2 - 4.7
-
isoelectric focusing
4.2
-
isoelectric focusing, 4 isoforms
4.25
-
isoelectric focusing, 4 isoforms
4.35
-
isoelectric focusing, 4 isoforms
4.45
-
isoelectric focusing, 4 isoforms
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
-
SDS-PAGE, PDHf, second band
61967
-
x * 61967, calculated from amino acid sequence
64000
-
x * 64000, deglycosylated protein, SDS-PAGE
65400
-
SDS-PAGE, gel filtration, MALDI MS
66500
-
SDS-PAGE, gel filtration, MALDI MS
67000
-
SDS-PAGE
76000
-
gel filtration
78000
-
1 * 78000, SDS-PAGE
79000
-
gel filtration
93000
-
x * 93000, recombinant enzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 61967, calculated from amino acid sequence; x * 64000, deglycosylated protein, SDS-PAGE; x * 93000, recombinant enzyme, SDS-PAGE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
microseeding, using 50 mM KH2PO4, 5% (v/v) 2-methyl-2,4-pentanediol, and 20% (w/v) polyethylene glycol 8000
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7 - 7.7
-
in potassium phosphate buffer, half life of 126 days at pH 7.7
686685
7
-
80% maximum stability at pH 7
687883
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
50% activity loss after incubation at 50C for 50 h
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing in 50 mM sodium phosphate, 2.4 mg/ml, results in 90% loss of activity
-
repeated freezing and thawing, two cycles, in the absence of glycerol has no effect on PDH activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
20C, at least 14 days, stable
-
4C, purified enzyme, 100 mM sodium phosphate, pH 6, 7, 8 or 9, 100 days, stable
-
4C, purified PDH, 20 mM sodium phosphate buffer, pH 7, 0.02% ProClin 300, 2 months, no loss of activity
-
several month at 4, pH 6.5 in potassium phosphate buffer
-
stable for 3 months at 4C, in sodium phosphate buffer pH 7.0
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE Sepharose column chromatography and phenyl Sepharose column chromatography
-
EMD DEAE 650 S column chromatography and Source 15 PHE column chromatography
-
hydrophobic interaction chromatography and anion exchange chromatography
-
using ammonium sulfate fractionation and ion-exchange and hydrophobic interaction chromatography
-
using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography
using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography; using ammonium sulfate fractionation, ion-exchange and hydrophobic interaction chromatography
-
using DEAE-Sephacel anion exchange chromatography and FPLC on phenyl-superose HR 10/10, Mono Q HR 5/5 and superose 12 HR 30/10 chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Aspergillus nidulans and Aspergillus niger
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain CBS 7435
-
expression in Escherichia coli for sequencing; expression in Escherichia coli for sequencing
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
degradation
molecular biology
-
gene expression analysis using PCR, pdh1 expression is upregulated upon exhaustion of the carbon source and appears to be additionally regulated under conditions of oxygen limitation, PDH production is highest on cellobiose and very low on fructose; gene expression analysis using PCR, pdh2 is constitutively expressed, PDH production is highest on cellobiose and very low on fructose; gene expression analysis using PCR, pdh3 is constitutively expressed, PDH production is highest on cellobiose and very low on fructose
synthesis