Information on EC 1.1.99.27 - (R)-pantolactone dehydrogenase (flavin)

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The expected taxonomic range for this enzyme is: Nocardia asteroides

EC NUMBER
COMMENTARY hide
1.1.99.27
-
RECOMMENDED NAME
GeneOntology No.
(R)-pantolactone dehydrogenase (flavin)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-Pantolactone + acceptor = 2-dehydropantolactone + reduced acceptor
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-pantolactone:acceptor oxidoreductase (flavin-containing)
High specificity for (R)-pantolactone. Phenazine methosulfate (PMS) can act as acceptor. The enzyme has been studied in the bacterium Nocardia asteroides and shown to be membrane-bound and induced by 1,2-propanediol. The FMN cofactor is non-covalently bound.
CAS REGISTRY NUMBER
COMMENTARY hide
140879-14-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-Pantolactone + (Fe(CN)6)3-
2-Dehydropantonolactone + Fe(CN)64-
show the reaction diagram
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-
-
-
-
(R)-Pantolactone + 2,6-dichlorophenol-indophenol
2-Dehydropantonolactone + reduced 2,6-dichlorophenol-indophenol
show the reaction diagram
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-
-
-
-
(R)-Pantolactone + indonitrotetrazolium
2-Dehydropantonolactone + reduced indonitrotetrazolium
show the reaction diagram
-
-
-
-
-
(R)-Pantolactone + medola blue
2-Dehydropantonolactone + reduced medola blue
show the reaction diagram
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-
-
-
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(R)-Pantolactone + nitrotetrazolium blue
2-Dehydropantonolactone + reduced nitrotetrazolium blue
show the reaction diagram
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-
-
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(R)-Pantolactone + phenazine methosulfate
2-Dehydropantonolactone + reduced phenazine methosulfate
show the reaction diagram
-
highly specific for (R)-pantolactone
-
-
-
additional information
?
-
-
1,2-propanediol induces the enzyme
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
1,2-propanediol induces the enzyme
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
dependent on, noncovalently bound
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-pantolactone
-
-
2-Dehydropantonolactone
-
20 mM
5,5'-dithiobis(2-nitrobenzoate)
-
1 mM, 47% inhibition
Atebrin
-
1 mM, 32.6% inhibition
Barbital
-
1 mM, 54.5% inhibition
CoCl2
-
1 mM, 24.4% inhibition
Diphenylhydantoin
-
1 mM, 22.1% inhibition
iodoacetate
-
1 mM, 17.4% inhibition
MnCl2
-
1 mM, 86.2% inhibition
NaAsO2
-
1 mM, 41% inhibition
NaCN
-
1 mM, 51.9% inhibition
NaF
-
1 mM, 27.3% inhibition
NaN3
-
1 mM, 9.1% inhibition
NEM
-
1 mM, 33.8% inhibition
NH2OH*HCl
-
1 mM, 71.9% inhibition
PCMB
-
1 mM, 41.0% inhibition
riboflavin
-
1 mM, 74.9% inhibition
Semicarbazide
-
1 mM, 23.1% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Brij 35
-
activates, maximal stimulation at 1.0%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26.8
(R)-pantolactone
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 45
-
about 20% of maximal activity at 25°C and 45°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
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x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
600000
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above 600000 Da, HPLC gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 42000, SDS-PAGE of enzyme treated with 2-mercaptoethanol
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
-
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
pH 9.0-10.0, 30 min, stable
35
-
pH 7.0, 10 min, stable below
45
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pH 7.0, 10 min, 43.1% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Brij35 stabilizes
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some stabilization by glycerol and ethylene glycol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, pH 7.4, 0.1 U/ml enzyme, 0.01 M Tris-HCl, 0.1 mM dithiothreitol, 0.5% Brij35, 2.9% loss of activity after 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE