Information on EC 1.1.99.22 - glycerol dehydrogenase (acceptor)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.99.22
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RECOMMENDED NAME
GeneOntology No.
glycerol dehydrogenase (acceptor)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycerol + acceptor = glycerone + reduced acceptor
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
glycerol:acceptor 1-oxidoreductase
A quinoprotein. Also acts, more slowly, on a number of other polyols including D-sorbitol, D-arabitol, meso-erythritol, adonitol and propylene glycol.
CAS REGISTRY NUMBER
COMMENTARY hide
249285-11-8
EC 1.1.99.22 is undistinguishable from EC 1.1.1.6 in Chemical Abstracts
9028-14-2
EC 1.1.99.22 is undistinguishable from EC 1.1.1.6 in Chemical Abstracts
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain JM109
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Manually annotated by BRENDA team
strain JM109
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Manually annotated by BRENDA team
strain IFO 3260, maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
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Manually annotated by BRENDA team
strain THE42
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Manually annotated by BRENDA team
strain THE42
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
2-oxoglutarate + pyrroloquinoline quinol
show the reaction diagram
(S)-2-hydroxyglutarate + ubiquinone
2-oxoglutarate + ubiquinol
show the reaction diagram
adonitol + acceptor
?
show the reaction diagram
D-arabitol + acceptor
?
show the reaction diagram
D-arabitol + acceptor
D-xylulose + reduced acceptor
show the reaction diagram
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-
-
-
?
D-gluconate + acceptor
5-keto-D-gluconate + reduced acceptor
show the reaction diagram
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-
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?
D-mannitol + acceptor
D-fructose + reduced acceptor
show the reaction diagram
D-sorbitol + acceptor
L-sorbose + reduced acceptor
show the reaction diagram
galactitol + acceptor
?
show the reaction diagram
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i.e. dulcitol, 29% of activity with glycerol
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?
glycerol + acceptor
dihydroxyacetone + reduced acceptor
show the reaction diagram
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-
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?
glycerol + acceptor
glycerone + reduced acceptor
show the reaction diagram
glycerol + NAD+
dihydroxyacetone + NADH
show the reaction diagram
meso-erythritol + acceptor
?
show the reaction diagram
meso-erythritol + acceptor
L-erythrulose + reduced acceptor
show the reaction diagram
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?
propylene glycol + acceptor
?
show the reaction diagram
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54% of activity with glycerol
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?
ribitol + acceptor
? + reduced acceptor
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2-hydroxyglutarate + pyrroloquinoline quinone
2-oxoglutarate + pyrroloquinoline quinol
show the reaction diagram
(S)-2-hydroxyglutarate + ubiquinone
2-oxoglutarate + ubiquinol
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
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acts in vitro as acceptor together with phenazine methosulfate
phenazine methosulfate
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acts in vitro as acceptor together with 2,6-dichlorophenolindophenol, linked to electron-transport chain of the organism
pyrroloquinoline quinone
ubiquinone
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additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NH4+
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85% activation at 10 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
D-arabitol
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2.3 - 34
glycerol
19
meso-erythritol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
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in an aqueous buffer system
10.5
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in reverse micelles
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
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about 50% of activity at pH 5.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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temperature increase reduces the enzyme activity, which can be restores by pyroloquinoline quinone. Adaptation of the CHM43 strain at 38.5C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 10
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stable in the presence of glycerol and detergent
287791
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
amphoteric detergents, such as dimethyldodecylamineoxide, lauroylsarcosine, dodecylsulfobetaine, lauroylbetaine are effective solubilizers
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dimethyldodecylamineoxide, 0.5%, stabilizes
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glycerol stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, solubilized, stable for 6 months
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2-4C, diluted enzyme solution, stable for 2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity by anion exchange chromatography and Ni-affinity chromatography
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to homogeneity by Ni-affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21 as His-tag fusion protein
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expression in Escherichia coli BL21(DE3)pLysS as His-tag fusion protein
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gene sldBA, DNA and amino acid sequence determination and analysis
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genes sldB and sldA encoding the small and the large subunit of the enzyme
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information