Information on EC 1.1.99.13 - glucoside 3-dehydrogenase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.99.13
-
RECOMMENDED NAME
GeneOntology No.
glucoside 3-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sucrose + acceptor = 3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lactose degradation II
-
-
Starch and sucrose metabolism
-
-
sucrose degradation VII (sucrose 3-dehydrogenase)
-
-
metabolism of disaccharids
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-
SYSTEMATIC NAME
IUBMB Comments
D-aldohexoside:acceptor 3-oxidoreductase
A flavoprotein (FAD). The enzyme acts on D-glucose, D-galactose, D-glucosides and D-galactosides, but D-glucosides react more rapidly than D-galactosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-74-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain D649
-
-
Manually annotated by BRENDA team
strain D649
-
-
Manually annotated by BRENDA team
strain IAM 1525
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-glucitol + acceptor
1,3,5-dehydro-D-glucitol + reduced acceptor
show the reaction diagram
2-deoxy-D-galactose + acceptor
2-deoxy-3-dehydro-D-galactose + reduced acceptor
show the reaction diagram
-
-
-
-
?
2-deoxy-D-glucose + acceptor
2-deoxy-3-dehydro-D-glucose + reduced acceptor
show the reaction diagram
-
-
-
-
?
aldohexosides + acceptor
?
show the reaction diagram
-
reaction in sugar metabolism
-
-
?
alpha-methyl-D-glucoside + acceptor
3-keto-alpha-methyl-glucoside + reduced acceptor
show the reaction diagram
anhydro-1,6-D-glucose + acceptor
?
show the reaction diagram
-
-
-
-
?
arbutin + acceptor
?
show the reaction diagram
beta-melibiose + acceptor
?
show the reaction diagram
-
-
-
-
?
beta-methyl-D-glucoside + acceptor
3-keto-beta-methyl-D-glucoside + reduced acceptor
show the reaction diagram
-
-
-
?
cellobionate + acceptor
?
show the reaction diagram
-
-
-
-
?
cellobiose + acceptor
?
show the reaction diagram
D-galactose + acceptor
3-dehydro-D-galactose + reduced acceptor
show the reaction diagram
D-galactosides + acceptor
3-ketogalactosides + reduced acceptor
show the reaction diagram
-
-
-
?
D-glucosamine + acceptor
3-dehydro-D-glucosamine + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
3-dehydro-D-glucose + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
3-ketoglucose + reduced acceptor
show the reaction diagram
D-glucose 1-phosphate + acceptor
3-keto-D-glucose 1-phosphate + reduced acceptor
show the reaction diagram
D-glucosides + acceptor
3-ketoglucosides + reduced acceptor
show the reaction diagram
-
react more rapidly than galactosides, aldopentoses and methylpentoses are no substrates
-
?
D-mannose + acceptor
3-dehydro-D-mannose + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-sorbitol + acceptor
?
show the reaction diagram
-
-
-
-
?
D-xylose + acceptor
?
show the reaction diagram
-
-
-
-
?
L-arabinose + acceptor
?
show the reaction diagram
-
-
-
-
?
lactobionate + acceptor
?
show the reaction diagram
lactose + acceptor
?
show the reaction diagram
lactulose + acceptor
?
show the reaction diagram
-
-
-
-
?
leucrose + acceptor
?
show the reaction diagram
-
-
-
-
?
maltobionate + acceptor
?
show the reaction diagram
-
-
-
-
?
maltose + acceptor
?
show the reaction diagram
melezitose + acceptor
?
show the reaction diagram
-
-
-
-
?
methyl-alpha-D-galactoside + acceptor
?
show the reaction diagram
-
-
-
-
?
methyl-beta-D-glucose + acceptor
3-keto-methyl-beta-D-glucose + reduced acceptor
show the reaction diagram
methyl-beta-thiogalactose + acceptor
3-dehydro-methyl-beta-D-thiogalactose + reduced acceptor
show the reaction diagram
-
-
-
-
?
N-carbamoyl-beta-D-glucopyranosylamine + acceptor
?
show the reaction diagram
p-arbutine + acceptor
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl 1-thio-beta-D-glucoside + acceptor
p-nitrophenyl-1-thio-3-dehydro-beta-D-glucoside + reduced acceptor
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-glucoside + acceptor
p-nitrophenyl-3-dehydro-alpha-D-glucoside + reduced acceptor
show the reaction diagram
-
-
-
-
?
p-nitrophenylvalidamine + acceptor
p-nitrophenyl-3-ketovalidamine + reduced acceptor
show the reaction diagram
p-nitrophenylvalienamine + acceptor
p-nitrophenyl-3-ketovalienamine + reduced acceptor
show the reaction diagram
raffinose + acceptor
?
show the reaction diagram
-
-
-
-
?
salicin + acceptor
?
show the reaction diagram
-
-
-
-
?
sucrose + acceptor
3-dehydro-alpha-D-glucosyl-beta-D-fructofuranoside + reduced acceptor
show the reaction diagram
TDP-glucose + acceptor
TDT-3-dehydro-D-glucose + reduced acceptor
show the reaction diagram
-
-
-
-
?
trehalose + acceptor
?
show the reaction diagram
UDP-glucose + acceptor
UDP-3-dehydro-D-glucose + reduced acceptor
show the reaction diagram
validoxylamine A + acceptor
3-ketovalidoxylamine A + reduced acceptor
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
aldohexosides + acceptor
?
show the reaction diagram
-
reaction in sugar metabolism
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
non-heme iron sulfur protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cyanide
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70% inhibition at high concentration
L-arabinose
-
slight
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Atabrine
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activation at acidic pH-values
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
cellobiose
-
-
2.31
D-cellobiose
-
-
25 - 26.1
D-galactose
0.016 - 2.9
D-glucose
2.8 - 13.8
D-maltose
28
D-xylose
-
-
22.4
L-arabinose
-
-
0.21
Lactobionate
-
-
0.36
Maltobionate
-
-
0.038 - 12.6
Sucrose
1.1
validoxylamine A
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-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
141
D-cellobiose
Agaricus bisporus
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-
145
D-galactose
Agaricus bisporus
-
-
143
D-glucose
Agaricus bisporus
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-
154
D-maltose
Agaricus bisporus
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-
200
D-xylose
Agaricus bisporus
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-
239
L-arabinose
Agaricus bisporus
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59
Sucrose
Agaricus bisporus
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.56
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carbon source: alpha-methyl-D-glucoside
0.74
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carbon source: isopropyl-thiogalactopyranoside
0.86
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carbon source: lactose
2.94
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validoxylamine A
149
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carbon source: D-glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
lactose with phenazine methosulfate as acceptor
8 - 9
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phenazine methosulfate and 2,6-dichlorphenolindophenol as acceptor
9
-
phenazine methosulfate or 3-(4,5-dimethyl-2-thiazolyl)-2,5-di-phenyl-2H-tetrazolium as acceptor
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 7.2
-
85% of maximal activity at pH 5.2 and 7.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
enzyme assay
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
1 * 55000, SDS-PAGE
57000
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gel filtration
67000
-
-
68000
-
1 * 68000, SDS-PAGE
73000
-
gel filtration
85000
-
low speed sedimentation
270000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
tetramer
-
4 * 66000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
and below, labile, sucrose stabilizes
389892
7 - 9
-
stable
389892
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sucrose protects against inactivation in acidic media
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, stable for 2 weeks under nitrogen
-
frozen, half-life 1 week
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene encoding glucose 3-dehydrogenase cloned and expressed in Escherichia coli, open reading frame of 1686 nucleotides, FAD binding motif found in the N-terminal region of the gene
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genes of a 3-ketoglycoside pathway cloned and expressed in Escherichia coli
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Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
regeneration via electrochemical methods using potassium ferricyanide as an electron mediator
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis