Information on EC 1.1.99.12 - sorbose dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.99.12
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RECOMMENDED NAME
GeneOntology No.
sorbose dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-sorbose + acceptor = 5-dehydro-D-fructose + reduced acceptor
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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-
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reduction
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SYSTEMATIC NAME
IUBMB Comments
L-sorbose:acceptor 5-oxidoreductase
2,6-Dichloroindophenol can act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-86-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
strain T-100 isolated from japanese persimmon
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-butanol + acceptor
n-butanal + reduced acceptor
show the reaction diagram
-
strain DSM 4025, 69.4% of activity with 1-propanol
-
?
1-pentanol + acceptor
n-pentanal + reduced acceptor
show the reaction diagram
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strain DSM 4025, 49.8% of activity with 1-propanol
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?
1-propanol + acceptor
n-propanal + reduced acceptor
show the reaction diagram
-
-
-
?
2-butanol + acceptor
2-butanone + reduced acceptor
show the reaction diagram
-
strain DSM 4025, 87.4% of activity with 1-propanol
-
?
2-propanol + acceptor
acetone + reduced acceptor
show the reaction diagram
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strain DSM 4025, 78.2% of activity with 1-propanol
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?
D-fructose + acceptor
2-keto-D-gulonate + reduced acceptor
show the reaction diagram
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strain DSM 4025, 6.6% of activity with 1-propanol
-
?
D-galactose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
180% of activity with L-sorbose
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-
?
D-gluconate + 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
135% of activity with L-sorbose
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
382% of activity with L-sorbose
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-
?
D-glucose + acceptor
D-gluconic acid + reduced acceptor
show the reaction diagram
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strain DSM 4025, 25.3% of activity with 1-propanol
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?
D-mannitol + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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328% of activity with L-sorbose
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-
?
D-mannitol + acceptor
? + reduced acceptor
show the reaction diagram
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strain DSM 4025, 16.2% of activity with 1-propanol
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?
D-mannose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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190% of activity with L-sorbose
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-
?
D-sorbitol + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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728% of activity with L-sorbose
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-
?
D-sorbitol + acceptor
D-glucose + D-gluconic acid + L-sorbose + reduced acceptor
show the reaction diagram
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strain DSM 4025, 7.1% of activity with 1-propanol
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?
D-xylose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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355% of activity with L-sorbose
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?
DL-glyceraldehyde + acceptor
? + reduced acceptor
show the reaction diagram
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strain DSM 4025, 80.2% of activity with 1-propanol
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?
ethanol + acceptor
ethanal + reduced acceptor
show the reaction diagram
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strain DSM 4025, 88.6% of activity with 1-propanol
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?
glycerol + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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327% of activity with L-sorbose
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?
iso-erythritol + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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236% of activity with L-sorbose
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?
L-arabinose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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318% of activity with L-sorbose
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?
L-sorbose + 2,6-dichlorophenolindophenol
5-keto-D-fructose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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no activity with D-fructose, sucrose and dulcitol
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?
L-sorbose + 2,6-dichlorophenolindophenol
L-sorbosone + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
L-sorbose + acceptor
5-dehydro-D-fructose + reduced acceptor
show the reaction diagram
L-sorbose + acceptor
L-sorbosone + reduced acceptor
show the reaction diagram
L-sorbose + oxidized 2,6-dichlorophenolindophenol
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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-
-
-
?
L-sorbosone + acceptor
2-keto-L-gulonate + reduced acceptor
show the reaction diagram
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strain DSM 4025, 16.2% of activity with 1-propanol
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?
maltose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
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282% of activity with L-sorbose
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?
n-heptanol + acceptor
n-heptanal + reduced acceptor
show the reaction diagram
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strain DSM 4025, 47.7% of activity with 1-propanol
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?
n-hexanol + acceptor
n-hexanal + reduced acceptor
show the reaction diagram
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strain DSM 4025, 49.1% of activity with 1-propanol
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?
polyethylene glycol 100 + acceptor
?
show the reaction diagram
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strain DSM 4025, 68.9% of activity with 1-propanol
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?
polyvinyl alcohol + acceptor
oxidized polyvinyl alcohol + reduced acceptor
show the reaction diagram
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strain DSM 4025, 38.1% of activity with 1-propanol
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?
propionaldehyde + acceptor
propanoate + reduced acceptor
show the reaction diagram
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strain DSM 4025, 83.3% of activity with 1-propanol
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-sorbose + 2,6-dichlorophenolindophenol
5-keto-D-fructose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
no activity with D-fructose, sucrose and dulcitol
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?
L-sorbose + acceptor
5-dehydro-D-fructose + reduced acceptor
show the reaction diagram
L-sorbose + acceptor
L-sorbosone + reduced acceptor
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c
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strain DSM 4025, physiological electron acceptor
pyrroloquinoline quinone
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
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1 mM, complete inhibition
Citric acid
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-
CoCl2
EGTA
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strain DSM 4025, 5 mM, 89% inhibition
FeCl2
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strain DSM 4025, 5 mM, 89% inhibition
FeCl3
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strain DSM 4025, 5 mM, 97% inhibition
Hg2+
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1 mM, complete inhibition
NiSO4
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strain DSM 4025, 5 mM, 77% inhibition
p-chloromercuribenzoate
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1 mM, 40% inhibition
Phenylmercuric nitrate
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1 mM, 40% inhibition
Semicarbazide
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1 mM, 40% inhibition
ZnCl2
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strain DSM 4025, 5 mM, 90% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
azide
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1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor
cyanide
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1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
1-propanol
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strain DSM 4025
4.3 - 230
L-sorbose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.73
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strain DSM 4025
10.3
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promoters has strong activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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strain DSM 4025
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.7
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pH 5.0: about 40% of activity maximum, pH 8.7: about 30% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 40
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strain DSM 4025
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
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strain DSM 4025
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
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SDS-PAGE
58000
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x * 58000, SDS-PAGE
62500
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strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
64500
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strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
135000
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strain DSM 4025, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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30 min, 20°C, complete inactivation
287726
6 - 6.5
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30 min, 20°C, highest stability
287726
7.5
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30 min, 20°C, complete inactivation
287726
additional information
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strain DSM 4025, enzyme is stable in alkaline pH and unstable with increasing acidity
287728
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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5 min, complete inactivation
additional information
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strain DSM 4025, enzyme is stable at and below 30°C, but unstable above 40°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
strain DSM 4025, PEG 600, DEAE-Toyopearl, Q-Sepharose
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
constructs with and without sequences of promoters, identification of the transcription initiation site
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expressed in Escherichia coli JM109 cells
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expression in Escherichia coli, constructs with and without sequences of promoters, identification of the transcription initiation site
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strain T-100, expression in Escherichia coli and Gluconobacter oxydans G624
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry