Information on EC 1.1.9.1 - alcohol dehydrogenase (azurin)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.9.1
-
RECOMMENDED NAME
GeneOntology No.
alcohol dehydrogenase (azurin)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a primary alcohol + azurin = an aldehyde + reduced azurin
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alcohol:azurin oxidoreductase
A soluble, periplasmic PQQ-containing quinohemoprotein. Also contains a single heme c. Occurs in Comamonas and Pseudomonas. Does not require an amine activator. Oxidizes a wide range of primary and secondary alcohols, and also aldehydes and large substrates such as sterols; methanol is not a substrate. Usually assayed with phenazine methosulfate or ferricyanide. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed ‘propeller’ structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-2-butanol + ferricyanide
butan-2-one + ferrocyanide
show the reaction diagram
-
enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively
-
-
?
(R)-2-heptanol + ferricyanide
heptan-2-one + ferrocyanide
show the reaction diagram
-
enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 5.5% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively
-
-
?
(R)-2-hexanol + ferrocyanide
hexan-2-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively
-
-
?
(R)-2-octanol + ferrocyanide
octan-2-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 8% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively
-
-
?
(R)-3-bromo-2-methyl-1-propanol + ferricyanide
(R)-3-bromo-2-methyl-propanal + ferrocyanide
show the reaction diagram
-
-
-
-
r
(R)-glycidol + ferrocyanide
? + ferricyanide
show the reaction diagram
-
enzyme electrode, 37% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no distinction
-
-
?
(R)-solketal + 2 ferricyanide
(R)-solketal aldehyde + 2 ferrocyanide + 2 H+
show the reaction diagram
-
(R)-enantiomer is prefered, enantiomeric ratio is 30
-
-
r
(R)-solketal + ferrocyanide
? + ferricyanide
show the reaction diagram
-
enzyme electrode, 35% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively
-
-
?
(R)-solketal aldehyde + ferrocyanide
(R)-solketal + ferricyanide
show the reaction diagram
(S)-2-butanol + ferricyanide
butan-2-one + ferrocyanide
show the reaction diagram
-
enzyme electrode, 14% of vmax with 1-butanol, soluble enzyme, 13% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 13fold preferred over (R)-enantiomer, with soluble enzyme 13.5fold, respectively
-
-
?
(S)-2-heptanol + ferricyanide
heptan-2-one + ferrocyanide
show the reaction diagram
-
enzyme electrode, 70% of vmax with 1-butanol, soluble enzyme, 63% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 315fold, respectively
-
-
?
(S)-2-hexanol + ferrocyanide
hexan-2-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 74% of vmax with 1-butanol, soluble enzyme, 71% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 35fold preferred over (R)-enantiomer, with soluble enzyme 105fold, respectively
-
-
?
(S)-2-octanol + ferrocyanide
octan-2-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 82% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 82fold preferred over (R)-enantiomer, with soluble enzyme 800fold, respectively
-
-
?
(S)-glycidol + ferrocyanide
? + ferricyanide
show the reaction diagram
-
enzyme electrode, 30% of vmax with 1-butanol, soluble enzyme, 1.6% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 0.9fold preferred over (R)-enantiomer, with soluble enzyme no disticnction
-
-
?
(S)-solketal + 2 ferricyanide
(S)-solketal aldehyde + 2 ferrocyanide + 2 H+
show the reaction diagram
-
the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis
-
-
r
(S)-solketal + ferrocyanide
? + ferricyanide
show the reaction diagram
-
enzyme electrode, 9% of vmax with 1-butanol, 0.7% of vmax with 1-butanol. With enzyme electrode, (S)-enantiomer is 39fold preferred over (R)-enantiomer, with soluble enzyme 117fold, respectively
-
-
?
1,2-butanediol + ferricyanide
2-hydroxybutanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol
-
-
?
1,2-cyclohexanediol + ferricyanide
2-hydroxycyclohexanone + ferrocyanide
show the reaction diagram
-
-
-
-
?
1,2-ethanediol + ferricyanide
hydroxyacetaldehyde + ferrocyanide
show the reaction diagram
-
ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol
-
-
?
1,2-propanediol + ferricyanide
2-hydroxypropanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity
-
-
?
1,3-butanediol + ferricyanide
3-hydroxybutanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol
-
-
?
1,3-butanediol + ferricyanide
? + ferrocyanide
show the reaction diagram
-
enzyme electrode, 91% of vmax with 1-butanol
-
-
?
1,3-cyclohexanediol + ferricyanide
3-hydroxycyclohexanone + ferrocyanide
show the reaction diagram
-
-
-
-
?
1,3-propandione + ferricyanide
3-hydroxypropanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol
-
-
?
1,4-butanediol + ferricyanide
4-hydroxybutanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol
-
-
?
1-butanol + ferricyanide
butanal + ferrocyanide
show the reaction diagram
1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine
butanal + ?
show the reaction diagram
-
-
-
-
r
1-heptanol + ferricyanide
heptanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol
-
-
?
1-hexanol + ferricyanide
hexanal + ferrocyanide
show the reaction diagram
-
ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol
-
-
?
1-octanol + ferricyanide
octanal + ferrocyanide
show the reaction diagram
1-octanol + ferrocyanide
octanal + ferricyanide
show the reaction diagram
-
enzyme electrode, 95% of vmax with 1-butanol, soluble enzyme, 95% of vmax with 1-butanol
-
-
?
1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine
octanal + ?
show the reaction diagram
-
-
-
-
r
1-pentanol + ferricyanide
pentanal + ferrocyanide
show the reaction diagram
1-pentanol + ferrocyanide
pentanal + ferricyanide
show the reaction diagram
-
enzyme electrode, 93% of vmax with 1-butanol, soluble enzyme, 100% of vmax with 1-butanol
-
-
?
1-pentanol + N,N,N',N'-tetramethyl-p-phenylenediamine
pentanal + ?
show the reaction diagram
-
-
-
-
r
1-propanol + ferricyanide
propanal + ferrocyanide
show the reaction diagram
1-propanol + N,N,N',N'-tetramethyl-p-phenylenediamine
propanal + ?
show the reaction diagram
-
-
-
-
r
2,3-butanediol + ferricyanide
3-hydroxybutan-2-one + ferrocyanide
show the reaction diagram
-
ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol
-
-
?
2,4-pentanediol + ferricyanide
4-hydroxypentan-2-one + ferrocyanide
show the reaction diagram
-
ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol
-
-
?
2-butanol + ferricyanide
butanone + ferrocyanide
show the reaction diagram
2-butanol + ferrocyanide
butanone + ferricyanide
show the reaction diagram
-
enzyme electrode, 17% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
-
-
?
2-hexanol + ferricyanide
hexan-2-one + ferrocyanide
show the reaction diagram
-
ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol
-
-
?
2-methylcyclohexanol + ferricyanide
2-methylcyclohexanone + ferrocyanide
show the reaction diagram
-
-
-
-
?
2-octanol + ferricyanide
octan-2-one + ferrocyanide
show the reaction diagram
-
-
-
-
?
2-octanol + ferrocyanide
octan-2-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 84% of vmax with 1-butanol, soluble enzyme, 80% of vmax with 1-butanol
-
-
?
2-pentanol + ferricyanide
pentan-2-one + ferrocyanide
show the reaction diagram
2-pentanol + ferrocyanide
pentan-2-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 40% of vmax with 1-butanol, soluble enzyme, 34% of vmax with 1-butanol
-
-
?
2-propanol + ferricyanide
acetone + ferrocyanide
show the reaction diagram
2-propanol + ferrocyanide
acetone + ferricyanide
show the reaction diagram
-
enzyme electrode, 9% of vmax with 1-butanol, soluble enzyme, 6% of vmax with 1-butanol
-
-
?
3-heptanol + ferricyanide
heptan-3-one + ferrocyanide
show the reaction diagram
-
-
-
-
?
3-heptanol + ferrocyanide
heptan-3-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 3.7% of vmax with 1-butanol, soluble enzyme, 1% of vmax with 1-butanol
-
-
?
3-hexanol + ferricyanide
hexan-3-one + ferrocyanide
show the reaction diagram
-
-
-
-
?
3-hexanol + ferrocyanide
hexan-3-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 6.5% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol
-
-
?
3-methyl-2-pentanol + ferricyanide
3-methylpentan-2-one + ferrocyanide
show the reaction diagram
3-methylcyclohexanol + ferricyanide
3-methylcyclohexanone + ferrocyanide
show the reaction diagram
-
-
-
-
?
3-nonanol + ferricyanide
nonan-3-one + ferrocyanide
show the reaction diagram
3-octanol + ferricyanide
octan-3-one + ferrocyanide
show the reaction diagram
-
-
-
-
?
3-octanol + ferrocyanide
octan-3-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 10% of vmax with 1-butanol, soluble enzyme, 10% of vmax with 1-butanol
-
-
?
3-pentanol + ferricyanide
pentan-3-one + ferrocyanide
show the reaction diagram
3-pentanol + ferrocyanide
pentan-3-one + ferricyanide
show the reaction diagram
-
enzyme electrode, 1% of vmax with 1-butanol, soluble enzyme, 1.4% of vmax with 1-butanol
-
-
?
4-decanol + ferricyanide
decan-4-one + ferrocyanide
show the reaction diagram
4-heptanol + ferricyanide
heptan-4-one + ferrocyanide
show the reaction diagram
4-methyl-2-pentanol + ferricyanide
4-methylpentan-2-one + ferrocyanide
show the reaction diagram
-
enzyme electrode, 5.6% of vmax with 1-butanol
-
-
?
6-aminohexan-1-ol + N,N,N',N'-tetramethyl-p-phenylenediamine
6-aminohexanal + ?
show the reaction diagram
-
-
-
-
r
acetaldehyde + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine
show the reaction diagram
-
-
-
-
r
allyl alcohol + ferricyanide
prop-2-enal + ferrocyanide
show the reaction diagram
-
ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol
-
-
?
benzyl alcohol + N,N,N',N'-tetramethyl-p-phenylenediamine
benzaldehyde + ?
show the reaction diagram
-
-
-
-
r
butanal + ferrocyanide
1-butanol + ferricyanide
show the reaction diagram
-
ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol
-
-
?
butanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine
show the reaction diagram
-
-
-
-
r
butane-1,3-diol + N,N,N',N'-tetramethyl-p-phenylenediamine
?
show the reaction diagram
-
-
-
-
r
cyclohexanol + ferricyanide
cyclohexanal + ferrocyanide
show the reaction diagram
-
-
-
-
?
cyclohexanol + ferricyanide
cyclohexanone + ferrocyanide
show the reaction diagram
-
enzyme electrode, 11% of vmax with 1-butanol, soluble enzyme, 9% of vmax with 1-butanol
-
-
?
ethanal + ferrocyanide
ethanol + ferricyanide
show the reaction diagram
ethanol + cytochrome c
ethanal + reduced cytochrome c
show the reaction diagram
-
5% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 60% of the activity with Wurster's Blue
-
-
r
ethanol + ferricyanide
ethanal + ferrocyanide
show the reaction diagram
ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine
ethanal + ?
show the reaction diagram
-
i.e. Wurster's Blue
-
-
?
ethanol + oxidized 2,6-dichlorophenolindophenol
ethanal + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
10% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 75% of the activity with Wurster's Blue
-
-
r
formaldehyde + N,N,N',N'-tetramethyl-p-phenylenediamine
methanol + ?
show the reaction diagram
-
-
-
-
r
glycerol + ferrocyanide
2,3-dihydroxypropanal + ferricyanide
show the reaction diagram
-
ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol
-
-
?
iso-amyl alcohol + ferricyanide
3-methylbutanol + ferrocyanide
show the reaction diagram
-
ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol
-
-
?
n-butanol + ferricyanide
butanal + ferrocyanide
show the reaction diagram
-
-
-
-
?
octanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine
show the reaction diagram
-
-
-
-
r
propanal + ferrocyanide
1-propanol + ferricyanide
show the reaction diagram
-
ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol
-
-
?
rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane + ferricyanide
? + ferrocyanide
show the reaction diagram
-
i.e. solketal. Enantiomeric ratio is 30 for solketal and 6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4-2H]dioxolane, d5-solketal. Isotopic substitution affects the relative kinetic weights of the initial hydron/deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heme c
-
both isoforms ADH IIB and ADH IIG
pyrroloquinoline quinone
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
28 - 30
(R)-2-butanol
0.7 - 1.1
(R)-2-heptanol
0.6 - 1.2
(R)-2-hexanol
0.4
(R)-2-octanol
-
enzyme electrode, pH 7.7, presence of CaCl2
302
(R)-3-bromo-2-methyl-1-propanol
-
pH 7.5, presence of CaCl2
4.8
(R)-glycidol
-
enzyme electrode, pH 7.7, presence of CaCl2
0.1 - 4
(R)-solketal
81
(R)-solketal aldehyde
-
pH 7.5, presence of CaCl2
4.5 - 5
(S)-2-butanol
0.04 - 0.06
(S)-2-heptanol
0.09
(S)-2-hexanol
-
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
0.05
(S)-2-octanol
-
enzyme electrode, pH 7.7, presence of CaCl2; soluble enzyme, pH 7.7, presence of CaCl2
4.5
(S)-glycidol
-
enzyme electrode, pH 7.7, presence of CaCl2
0.99 - 1
(S)-solketal
182
(S)-solketal aldehyde
-
pH 7.5, presence of CaCl2
0.226 - 10.2
1,2-propanediol
0.3
1,3-butanediol
-
enzyme electrode, pH 7.7, presence of CaCl2
50
1,3-cyclohexandiol
-
pH 7.5, 25°C
0.005 - 0.15
1-butanol
0.005 - 0.006
1-Octanol
0.005 - 0.006
1-Pentanol
0.06
1-propanol
8 - 12
2-butanol
81
2-Methylcyclohexanol
-
pH 7.5, 25°C
0.05
2-Octanol
0.4 - 0.6
2-Pentanol
22 - 30
2-propanol
2.9 - 4.3
3-Heptanol
5.3 - 6.3
3-Hexanol
0.9
3-methyl-2-pentanol
-
enzyme electrode, pH 7.7, presence of CaCl2
0.11
3-Methylcyclohexanol
-
pH 7.5, 25°C
0.8
3-nonanol
0.9
3-octanol
28 - 30
3-Pentanol
0.4
4-decanol
0.7
4-heptanol
0.75
4-methyl-2-pentanol
-
enzyme electrode, pH 7.7, presence of CaCl2
0.24
5-methyl-2-pentanol
-
enzyme electrode, pH 7.7, presence of CaCl2
0.2
6-aminohexan-1-ol
-
pH 7.0, 20°C
0.9
acetaldehyde
-
pH 7.0, 20°C
0.001
benzyl alcohol
-
pH 7.0, 20°C
0.1
Butanal
-
pH 7.0, 20°C
0.28
Butane-1,3-diol
-
pH 7.0, 20°C
0.8 - 0.83
Cyclohexanol
0.7 - 197
Ethanal
2.2 - 211
ethanol
3
formaldehyde
-
pH 7.0, 20°C
2.4
glycerol
-
isoform ADH IIG, pH 8.0
0.04
octanal
-
pH 7.0, 20°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.3
(R)-3-bromo-2-methyl-1-propanol
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
2.12
(R)-solketal
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
8.3
(R)-solketal aldehyde
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
0.18
(S)-solketal
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
17.1
(S)-solketal aldehyde
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
19.9
Ethanal
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
17.5 - 17.9
ethanol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
67
(R)-3-bromo-2-methyl-1-propanol
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
40031
517
(R)-solketal
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
12074
102
(R)-solketal aldehyde
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
27221
94
(S)-solketal aldehyde
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
40032
101
Ethanal
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
2141
85
ethanol
Comamonas testosteroni
-
pH 7.5, presence of CaCl2
69
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14
-
isoform ADH IIG, pH 9.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
-
1 * 65000, SDS-PAGE
69000
-
ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE
70000
-
gel filtration
72000
-
ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE
72630
x * 72630, calculated, x * 73262, MALDI-TOF including heme c
73200
-
1 * 71000, SDS-PAGE, 1 * 73200, calculated
73262
x * 72630, calculated, x * 73262, MALDI-TOF including heme c
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 72630, calculated, x * 73262, MALDI-TOF including heme c
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to 1.44 A resolution. The N-terminal domain has a beta-propeller fold and binds one pyrroloquinoline quinone cofactor and one calcium ion in the active site. A tetrahydrofuran-2-carboxylic acid molecule is present in the substrate-binding cleft. The C-terminal domain is an -helical type I cytochrome c with His608 and Met647 as heme-iron ligands. An unusual disulfide bond between two adjacent cysteines bridges the redox centers. It appears essential for electron transfer. A water channel delineates a possible pathway for proton transfer from the active site to the solvent
to 2.4 A resolution, space group C2
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, storage for prolonged periods without loss of activity
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4°C, 0.15 M potassium phosphate buffer, pH 7.0, storage for routine purposes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from ethanol-grown cells
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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co-immobilization of enzyme with redox polymer poly(vinylpyridine) complex functionalized with osmium bis(bipyridine) chloride on an electrode. The enzyme electrode readily oxidizes primary alcohols and secondary alcohols with maximum current densities varying between 0.43 and 0.98 A per m2 depending on the substrate and the operation temperature. The enzyme electrode is enantioselective in the oxidation of secondary alcohols. A strong preference is observed for the (S)-2-alcohols, the enantioselectivity increases with increasing chain length. The enantiomeric ratio E increases from 13 for (R,S)-2-butanol to approximately 80 for (R,S)-2-heptanol and (R,S)-2-octanol