Information on EC 1.1.5.B3 - quinone dependent L-lactate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.5.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
quinone dependent L-lactate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-lactate + quinone = pyruvate + quinol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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SYSTEMATIC NAME
IUBMB Comments
L-lactate:quinone oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ATCC 621
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Manually annotated by BRENDA team
strain 33, DSM 3504 and ATCC 15163
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-lactate + 2,6-dichlorophenol indophenol
pyruvate + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
L-lactate + oxidized 2,6-dichlorophenol indophenol
pyruvate + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
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stereospecific for L-lactate
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?
L-lactate + oxidized 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lactate + oxidized 2,6-dichlorophenolindophenol
pyruvate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dichlorophenol indophenol
2,6-dichlorophenolindophenol
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phenazine methosulfate
pyrroloquinoline quinone
quinone
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dependent on
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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the enzyme expression is supressed/regulated by the FadR-type regulator LldR, encoded by NCgl2814, binds to the upstream region of NCgl2816-lldD, L-lactate prevents binding of LldRHis to the NCgl2816-lldD promoter region
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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the enzyme expression is induced by a temperature shift and by growth on L-lactate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.51
L-lactate
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pH 7.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
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wild-type strain ATCC 13032, without L-lactate
0.14
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wild-type strain, cell extract
0.23
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wild-type strain ATCC 13032, with L-lactate
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme from membranes by solubilization with Triton X-100 and anion exchange chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene ldhD, DNA microarray expression analysis, gene ldhD acts in an operon with the permease gene NCgl2816
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology