Information on EC 1.1.3.5 - hexose oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.3.5
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RECOMMENDED NAME
GeneOntology No.
hexose oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose + O2 = D-glucono-1,5-lactone + H2O2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pentose phosphate pathway
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
D-hexose:oxygen 1-oxidoreductase
A copper glycoprotein. Also oxidizes D-galactose, D-mannose, maltose, lactose and cellobiose.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-75-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
orange
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Manually annotated by BRENDA team
Citrus vulgaris
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-
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Manually annotated by BRENDA team
red alga
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-
Manually annotated by BRENDA team
red alga
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-galactose + O2
2-deoxy-D-galactono-1,5-lactone + H2O2
show the reaction diagram
2-deoxy-D-glucose + O2
2-deoxy-D-glucono-1,5-lactone + H2O2
show the reaction diagram
cellobiose + O2
4-O-(beta-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
D-2-glucosamine + O2
2-amine-D-glucono-1,5-lactone + H2O2
show the reaction diagram
D-galactose + O2
D-galactono-1,5-lactone + H2O2
show the reaction diagram
D-glucose + O2
D-glucono-1,5-lactone + H2O2
show the reaction diagram
D-glucose-6-phosphate + O2
D-glucono-1,5-lactone-6-phosphate + H2O2
show the reaction diagram
D-mannose + O2
D-mannono-1,5-lactone + H2O2
show the reaction diagram
D-xylose + O2
D-xylono-1,4-lactone
show the reaction diagram
lactose + O2
4-O-(beta-D-galactopyranosido)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
maltose + O2
4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
show the reaction diagram
additional information
?
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hexose oxidase transforms a number of mono- and oligosaccharides such as beta-D-glucose, D-galactose, xylose, arabinose, cellobiose, lactose, maltose, maltotriose and maltotetraose to corresponding lactones, concomitant with the formation of H2O2, which is used for improving of on rheological and bread making properties of flour, hexose oxidase is used separately in each corn bran-wheat flour formula with L-ascorbic acid at 75 mg/kg, glucose at 0.5% and vital gluten at 9.2%
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose + O2
D-glucono-1,5-lactone + H2O2
show the reaction diagram
additional information
?
-
-
hexose oxidase transforms a number of mono- and oligosaccharides such as beta-D-glucose, D-galactose, xylose, arabinose, cellobiose, lactose, maltose, maltotriose and maltotetraose to corresponding lactones, concomitant with the formation of H2O2, which is used for improving of on rheological and bread making properties of flour, hexose oxidase is used separately in each corn bran-wheat flour formula with L-ascorbic acid at 75 mg/kg, glucose at 0.5% and vital gluten at 9.2%
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
Benzoate
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diethyldithiocarbamate
Galacturonic acids
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glucuronic acid
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hydroxylamine
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Propionate
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pyruvate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
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100 mM, 1.5fold increase in activity
NaCl
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20 mM, 1.4fold increase in activity
additional information
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enzyme seems to be activated by proteolytic cleavage of full length protein, remaining polypeptide chains are physically linked together
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.5
cellobiose
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3.8 - 20
D-galactose
2.5 - 8.5
D-glucose
1.7
lactose
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28
maltose
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
broad pH range, two optima can be distinguished
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
87000
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native PAGE on gradient gel
110000
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gel filtration
117000
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gel filtration
130000
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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slow denaturation above
287637
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50 - 60
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not stable above
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very stable enzyme, no activity decrease after storage at room temperature overnight
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 7 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Sephadex G-200
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DEAE-Sepharose, Sephacryl S-200, phenyl-Sepharose, chromatofocusing, native and recombinant enzyme
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perfusion chromatography (Poros DEAE-50, Poros HP2, Poros HQ), Sephacryl S200
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Q-Sepharose, Phenyl-sepharose, Superdex 200
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry
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the enzyme is useful for improving the rheological and bread making properties of flour and the quality of the bread, overview
nutrition