Information on EC 1.1.3.29 - N-acylhexosamine oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.3.29
-
RECOMMENDED NAME
GeneOntology No.
N-acylhexosamine oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acetyl-D-glucosamine + O2 = N-acetyl-D-glucosaminate + H2O2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-acyl-D-hexosamine:oxygen 1-oxidoreductase
Also acts on N-glycolylglucosamine, N-acetylgalactosamine and, more slowly, on N-acetylmannosamine.
CAS REGISTRY NUMBER
COMMENTARY hide
121479-58-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 15-1
-
-
Manually annotated by BRENDA team
strain 15-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-galactosamine + O2
?
show the reaction diagram
-
35% of the activity with N-acetyl-D-glucosamine
-
-
?
D-galactose + O2
D-galactonelactone + H2O2
show the reaction diagram
-
0.43% of the activity with N-acetyl-D-glucosamine
-
-
?
D-glucosamine + O2
?
show the reaction diagram
-
14% of the activity with N-acetyl-D-glucosamine
-
-
?
D-glucose + O2
D-gluconolactone + H2O2
show the reaction diagram
-
0.3% of the activity with N-acetyl-D-glucosamine
-
-
?
D-mannosamine + O2
?
show the reaction diagram
-
0.03% of the activity with N-acetyl-D-glucosamine
-
-
?
D-mannose + O2
D-mannonolactone + H2O2
show the reaction diagram
-
0.38% of the activity with N-acetyl-D-glucosamine
-
-
?
N,N-diacetylchitobiose + O2
?
show the reaction diagram
-
31% of the activity with N-acetyl-D-glucosamine
-
-
?
N-acetyl-beta-D-glucosamine + O2
N-acetyl-D-glucosaminolactone + H2O2
show the reaction diagram
N-acetyl-D-galactosamine + O2
N-acetyl-D-galactosaminolactone + H2O2
show the reaction diagram
N-acetyl-D-mannosamine + O2
N-acetyl-D-mannosaminolactone + H2O2
show the reaction diagram
N-acetylmuramic acid + O2
?
show the reaction diagram
N-glycolyl-D-glucosamine + O2
N-glycolyl-D-glucosaminolactone + H2O2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
probably contains unidentified flavin component
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cd2+
-
weak
oxalate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SDS
-
slightly accelerates activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
D-galactosamine
-
-
170
D-galactose
-
-
40
D-glucosamine
-
-
290
D-glucose
-
-
59
D-mannose
-
-
18
N,N-diacetylchitobiose
-
-
33
N-acetyl muramic acid
-
-
0.1
N-acetyl-D-galactosamine
-
-
0.24
N-acetyl-D-glucosamine
-
-
40
N-acetyl-D-mannosamine
-
-
2.6
N-Glycolyl-D-glucosamine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70.4
-
N-acetyl-D-glucosamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11
-
pH 5.0: about 70% of maximal activity, pH 11: about 50% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
170000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 9
-
stable at 45°C for 10 min
389762
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
10 min, stable
70
-
100% remaining activity after 10 min
83
-
50% remaining activity after 10 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis