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Information on EC 1.1.3.23 - thiamine oxidase Word Map on EC 1.1.3.23
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The enzyme appears in viruses and cellular organisms
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thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2
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thiamine:oxygen 5-oxidoreductase
A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.
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thiamin dehydrogenase
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thiamin:oxygen 5-oxidoreductase
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thiamine dehydrogenase
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unidentified soil bacterium
ATCC 25589
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brenda
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oxythiamine + O2
? + H2O2
pyrithiamine + O2
? + H2O2
thiamine + O2
?
unidentified soil bacterium
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thiamine + O2
thiamine acetate + H2O2
oxythiamine + O2
? + H2O2
unidentified soil bacterium
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?
oxythiamine + O2
? + H2O2
unidentified soil bacterium
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?
oxythiamine + O2
? + H2O2
unidentified soil bacterium
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?
pyrithiamine + O2
? + H2O2
unidentified soil bacterium
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?
pyrithiamine + O2
? + H2O2
unidentified soil bacterium
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?
pyrithiamine + O2
? + H2O2
unidentified soil bacterium
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?
thiamine + O2
thiamine acetate + H2O2
unidentified soil bacterium
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?
thiamine + O2
thiamine acetate + H2O2
unidentified soil bacterium
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?
thiamine + O2
thiamine acetate + H2O2
unidentified soil bacterium
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reaction via an aldehyde intermediate which is not released
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ir
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thiamine + O2
?
unidentified soil bacterium
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FAD
unidentified soil bacterium
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FAD
unidentified soil bacterium
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8-alpha-N(1)-histidyl-FAD
FAD
unidentified soil bacterium
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1 mol/mol enzyme; 8-alpha-N(1)-histidyl-FAD
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Cu2+
unidentified soil bacterium
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Hg2+
unidentified soil bacterium
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p-Chloromeruribenzoate
unidentified soil bacterium
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pyrithiamine
unidentified soil bacterium
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Quinacrine
unidentified soil bacterium
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Semicarbazide
unidentified soil bacterium
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1.72
thiamine
unidentified soil bacterium
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-
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5
H+
unidentified soil bacterium
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release of H+
1.67
thiamine
unidentified soil bacterium
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thiamine acetate formed
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2.18
unidentified soil bacterium
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additional information
unidentified soil bacterium
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additional information
unidentified soil bacterium
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8 - 9
unidentified soil bacterium
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41500
unidentified soil bacterium
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gel filtration
52000
unidentified soil bacterium
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gel filtration
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monomer
unidentified soil bacterium
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1 * 49000-50000, SDS-PAGE
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50
unidentified soil bacterium
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stable for 30 min
65
unidentified soil bacterium
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not stable above
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-70°C, 3 months
unidentified soil bacterium
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4°C, 1 week
unidentified soil bacterium
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unidentified soil bacterium
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Neal, R.A.
Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2-methyl-4-amino-5-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism
J. Biol. Chem.
245
2599-2604
1970
unidentified soil bacterium
brenda
Gomez-Moreno, C.; Choy, M.; Edmondson, D.E.
Purification and properties of the bacterial flavoprotein: thiamin dehydrogenase
J. Biol. Chem.
254
7630-7635
1979
unidentified soil bacterium
brenda
Gomez-Moreno, C.; Edmondson, D.E.
Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase
Arch. Biochem. Biophys.
239
46-52
1985
unidentified soil bacterium
brenda
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