Information on EC 1.1.3.10 - pyranose oxidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.3.10
-
RECOMMENDED NAME
GeneOntology No.
pyranose oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose + O2 = 2-dehydro-D-glucose + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
pyranose:oxygen 2-oxidoreductase
A flavoprotein (FAD). Also oxidizes D-xylose, L-sorbose and D-glucono-1,5-lactone, which have the same ring conformation and configuration at C-2, C-3 and C-4.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-80-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
unidentified
-
-
Manually annotated by BRENDA team
Coriolus sp.
-
-
-
Manually annotated by BRENDA team
Corticium caeruleum
-
-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
Phanerochaete gigantea
-
-
-
Manually annotated by BRENDA team
Polyporus obtusus
-
-
-
Manually annotated by BRENDA team
Polyporus obtusus AU124PD
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-
-
Manually annotated by BRENDA team
Saxidomus giganteus
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Trametes cinnabarinus
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
-
pyranose 2-oxidase, rather than glucose 1-oxidase, represents a major source of sugar-derived H2O2 under the culture conditions used
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-fructose + O2
1,5-anhydro-3-keto-D-fructose + H2O2
show the reaction diagram
-
-
yield of 1,5-anhydro-3-keto-D-fructose: 33%
?
1,5-anhydro-D-glucitol + O2
1,5-anhydro-D-fructose + H2O2
show the reaction diagram
Phanerochaete gigantea
-
8% relative activity to D-glucose
yield of 1,5-anhydro-D-fructose: 100%
?
1,5-anhydro-D-glucitol + O2
?
show the reaction diagram
-
18.4% of the activity with D-glucose
-
-
?
1,5-anhydro-S-sorbitol + O2
?
show the reaction diagram
-
-
-
-
?
1,6-anhydro-D-glucose + O2
?
show the reaction diagram
-
-
-
-
?
1-beta-aurothioglucose + O2
?
show the reaction diagram
-
91% relative activity to D-glucose
-
-
?
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) + O2
? + H2O2
show the reaction diagram
-
-
-
?
2,6-dichloroindophenol + O2
? + H2O2
show the reaction diagram
-
-
-
?
2,6-dimethyl-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
2-chloro-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
-
-
-
?
2-deoxy-2-fluoro-D-glucose + O2
2-deoxy-3-dehydro-D-glucose + H2O2
show the reaction diagram
slow substrate
-
-
?
2-deoxy-D-galactose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
2-deoxy-D-glucose + O2
2-deoxy-3-keto-D-glucose + H2O2
show the reaction diagram
2-deoxy-D-glucose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
2-keto-D-glucose + O2
2,3-diketo-D-glucose + H2O2
show the reaction diagram
2-methoxy-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
-
-
-
?
3-deoxy-3-fluoro-beta-D-glucose + H2O2
?
show the reaction diagram
3-deoxy-D-glucose + O2
2-keto-3-deoxy-D-glucose + H2O2
show the reaction diagram
3-O-methyl-D-glucose + O2
2-keto-3-O-methyl-D-glucose + H2O2
show the reaction diagram
-
8.6% relative activity to D-glucose
-
?
5-thioglucose + O2
2-keto-5-thioglucose + H2O2
show the reaction diagram
6-deoxy-D-glucose + O2
2-keto-6-deoxy-D-glucose + H2O2
show the reaction diagram
allose + O2
?
show the reaction diagram
-
38% relative activity to D-glucose
-
-
?
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
alpha-D-melibiose + O2
? + H2O2
show the reaction diagram
beta-D-galactose + O2
2-dehydro-D-galactose + H2O2
show the reaction diagram
poor substrate
-
-
?
cellobiose + O2
2-dehydrocellobiose + H2O2
show the reaction diagram
-
17.8% activity compared to D-glucose
-
-
?
D-allose + O2
?
show the reaction diagram
D-arabinose + O2
?
show the reaction diagram
-
1.87% of the activity with D-glucose
-
-
?
D-cellobiose + O2
?
show the reaction diagram
D-fructose + O2
2-dehydro-D-fructose + H2O2
show the reaction diagram
D-fructose + O2
? + H2O2
show the reaction diagram
D-fucono-1,5-lactone + O2
2-keto-D-gluconate + D-araboascorbate + H2O2
show the reaction diagram
D-fucose + O2
?
show the reaction diagram
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + 1,4-hydroquinone
show the reaction diagram
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
show the reaction diagram
-
-
-
?
D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-galactose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
show the reaction diagram
-
-
-
-
?
D-galactose + ferrocenium hexafluorophosphate
2-dehydro-D-galactose + ferrocene
show the reaction diagram
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
show the reaction diagram
D-galactose + O2
?
show the reaction diagram
D-glucono-1,5-lactone + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-glucono-1,5-lactone
show the reaction diagram
D-glucono-1,5-lactone + O2
2-dehydro-D-glucono-1,5-lactone + H2O2
show the reaction diagram
-
107% activity compared to D-glucose
-
-
?
D-glucono-1,5-lactone + O2
?
show the reaction diagram
D-gluconolactone + O2
? + H2O2
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + 1,4-hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
show the reaction diagram
1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction
-
-
?
D-glucose + 1,4-benzoquinone
?
show the reaction diagram
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
2-dehydro-D-glucose + ?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical
2-dehydro-D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
show the reaction diagram
D-glucose + 2,6-dichloroindophenol
2-dehydro-D-glucose + ?
show the reaction diagram
-
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dichlorophenolindophenol
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2,6-dimethyl-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-methoxy-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-methyl-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-glucose + ?
show the reaction diagram
D-glucose + ferricenium hexafluorophosphate
?
show the reaction diagram
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocenium ion
show the reaction diagram
-
-
-
-
?
D-glucose + ferrocenium hexafluorophosphate
2-dehydro-D-glucose + ferrocene
show the reaction diagram
-
-
-
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ?
show the reaction diagram
-
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
D-glucose + O2
D-arabino-2-hexosulose + H2O2
show the reaction diagram
-
the enzyme is produced during glucose starvation
-
?
D-glucose + O2
D-arabino-hexos-2-ulose + H2O2
show the reaction diagram
D-glucose + O2
D-glucosone + H2O2
show the reaction diagram
D-glucose + tetrafluoro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-maltoheptaose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-maltopentaose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-maltose + O2
?
show the reaction diagram
-
15.1% of the activity with D-glucose
-
-
?
D-maltotriose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-mannoheptose + O2
?
show the reaction diagram
-
8.2% relative activity to D-glucose
-
-
?
D-mannose + O2
2-dehydro-D-mannose + H2O2
show the reaction diagram
-
18.4% activity compared to D-glucose
-
-
?
D-mannose + O2
2-keto-D-mannose + H2O2
show the reaction diagram
-
0.9% relative activity to D-glucose
-
?
D-mannose + O2
?
show the reaction diagram
-
6.02% of the activity with D-glucose
-
-
?
D-melibiose + O2
?
show the reaction diagram
D-ribose + 1,4-benzoquinone
2-dehydro-D-ribose + 1,4-hydroquinone
show the reaction diagram
-
-
-
-
?
D-ribose + O2
2-dehydro-D-ribose + H2O2
show the reaction diagram
-
-
-
-
?
D-trehalose + O2
?
show the reaction diagram
selectivity of pyranose 2-oxidase-based biosensor system for different sugar substrates analyzed
-
-
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + 1,4-hydroquinone
show the reaction diagram
-
-
-
-
?
D-xylose + 3-methyl-2-benzothiazolinone hydrazone
2-dehydro-D-xylose + ?
show the reaction diagram
D-xylose + O2
2-dehydro-D-xylose + H2O2
show the reaction diagram
D-xylose + O2
?
show the reaction diagram
D-xylose + O2
D-threo-pentos-2-ulose + H2O2
show the reaction diagram
D-xylose + O2
D-xylosone + H2O2
show the reaction diagram
ferricenium hexafluorophosphate + O2
? + H2O2
show the reaction diagram
-
-
-
?
gentibiose + O2
2-keto-D-gentibiose + H2O2
show the reaction diagram
L-arabinose + 1,4-benzoquinone
2-dehydro-L-arabinose + 1,4-hydroquinone
show the reaction diagram
-
-
-
-
?
L-arabinose + O2
2-dehydro-L-arabinose + H2O2
show the reaction diagram
-
-
-
-
?
L-arabinose + O2
?
show the reaction diagram
L-idose + O2
?
show the reaction diagram
-
-
-
-
?
L-sorbose + 3-methyl-2-benzothiazolinone hydrazone
?
show the reaction diagram
L-sorbose + O2
2-dehydro-L-sorbose + H2O2
show the reaction diagram
L-sorbose + O2
5-dehydro-D-fructose + H2O2
show the reaction diagram
L-sorbose + O2
?
show the reaction diagram
L-sorbose D-allose + O2
?
show the reaction diagram
-
-
-
?
lactose + O2
2-dehydrolactose + H2O2
show the reaction diagram
-
0.71% activity compared to D-glucose
-
-
?
maltose + O2
2-dehydro-D-maltose + H2O2
show the reaction diagram
-
92.2% activity compared to D-glucose
-
-
?
maltose + O2
? + H2O2
show the reaction diagram
melibiose + O2
?
show the reaction diagram
-
-
-
?
methyl alpha-D-glucoside + O2
2-dehydro-alpha-D-methylglucoside + H2O2
show the reaction diagram
methyl beta-D-glucoside + O2
2-dehydro-beta-D-methylglucoside + H2O2
show the reaction diagram
methyl-beta-D-glucoside + O2
?
show the reaction diagram
-
-
-
?
sucrose + O2
? + H2O2
show the reaction diagram
tetrafluoro-1,4-benzoquinone + O2
? + H2O2
show the reaction diagram
trehalose + O2
?
show the reaction diagram
-
14.3% of the activity with D-glucose
-
-
?
wheat flour + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
Coriolus sp.
-
-
-
-
?
D-galactose + O2
2-dehydro-D-galactose + H2O2
show the reaction diagram
-
mutants T169S, T169N, and T169G
-
-
?
D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
D-glucose + O2
D-arabino-2-hexosulose + H2O2
show the reaction diagram
-
the enzyme is produced during glucose starvation
-
?
D-xylose + O2
D-xylosone + H2O2
show the reaction diagram
Coriolus sp.
-
-
-
-
?
L-sorbose + O2
5-dehydro-D-fructose + H2O2
show the reaction diagram
Coriolus sp.
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
slightly stimulates at 1 mM
Co2+
-
slightly stimulates at 1 mM
Fe2+
-
stimulation
Mg2+
-
slightly stimulates at 1 mM
Zn2+
-
slightly stimulates at 1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-keto-D-glucose
-
5% reduction at 100 mM, 10% reduction at 200 mM, no deactivating effect determined
2-mercaptoethanol
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
8-hydroxyquinoline
acetate
alpha,alpha'-dipyridyl
ammonium sulfate
-
slightly inhibits
ascorbic acid
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
Co2+
-
13.3% inhibition
D-glucose
Polyporus obtusus
-
inhibits the oxidation of gluconolactone
D-xylose
Polyporus obtusus
-
inhibits the oxidation of gluconolactone
Dithionite
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
dithiothreitol
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
ethanol
Fe2+
-
completely inhibits at 1 mM
glutathione
Coriolus sp.
-
-
hydrogen peroxide
-
inactivation determined
L-sorbose
Polyporus obtusus
-
inhibits the oxidation of gluconolactone
Lead acetate
-
50% inhibition at 10 mM
Mn2+
-
50% inhibition at 10 mM
N-bromosuccinimide
NaCl
-
slightly inhibits at concentrations higher than 8%
Ni2+
-
28.6% inhibition
p-chloromercuribenzoate
propionate
-
-
reduced glutathione
-
10% inhibition at 1 mM, 95% inhibition at 10 mM
Sodium azide
-
slightly inhibitory at 1 mM, more than 85% activity remains
valerate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
catalase
-
the presence of catalase increases the rate of reaction
-
NaCl
-
up to 8%
additional information
-
an increase in the pressure with compressed air results in higher rates of bioconversion of D-glucose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0052 - 2
1,4-benzoquinone
10.7
1,5-anhydro-D-glucitol
-
pH 7.0, 37C
0.07 - 0.09
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical
-
0.051 - 0.39
2,6-dichloroindophenol
0.4 - 2.1
2,6-dimethyl-1,4-benzoquinone
0.31 - 0.48
2-chloro-1,4-benzoquinone
10.32
2-deoxy-D-galactose
100 mM ethanolamine buffer, pH 10.5
11.3 - 99.3
2-deoxy-D-glucose
0.21 - 0.42
2-methoxy-1,4-benzoquinone
0.11 - 0.51
2-methyl-1,4-benzoquinone
3.9
5-thioglucose
-
-
1 - 1.53
alpha-D-glucose
0.57 - 0.97
beta-D-glucose
290
cellobiose
-
at pH 6.5 and 25C
22.1 - 68
D-Allose
174
D-arabinose
-
pH 7.0, 37C
0.71
D-cellobiose
100 mM ethanolamine buffer, pH 10.5
103 - 2558
D-fructose
6.25
D-fucose
100 mM ethanolamine buffer, pH 10.5
0.093 - 1260
D-galactose
51.1
D-glucono-1,5-lactone
-
at pH 6.5 and 25C
38
D-gluconolactone
-
-
0.018 - 240
D-glucose
6.02
D-maltoheptaose
100 mM ethanolamine buffer, pH 10.5
6.14
D-maltopentaose
100 mM ethanolamine buffer, pH 10.5
329
D-maltose
-
pH 7.0, 37C
6.61
D-maltotriose
100 mM ethanolamine buffer, pH 10.5
110
D-mannoheptose
-
-
295 - 370
D-mannose
2 - 1500
D-melibiose
120
D-melibose
-
-
-
180
D-ribose
-
with 1,4-benzoquinone as electron acceptor, at pH 6.5 and 37C
7.51
D-trehalose
100 mM ethanolamine buffer, pH 10.5
2.4 - 78.4
D-xylose
0.092 - 1.12
ferricenium hexafluorophosphate
0.054 - 0.408
ferricenium ion
3.43
ferricyanide
-
substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30C, pH 6.5
0.015 - 0.4
ferrocenium hexafluorophosphate
0.187
ferrocenium ion
using D-glucose as cosubstrate, at 30C, pH 6.5
62
gentibiose
-
-
0.79 - 0.85
glucose
1.55 - 97
L-arabinose
2.9 - 176
L-sorbose
46.4 - 257
maltose
72.8
melibiose
at 30C, pH 6.5
0.35 - 0.4
methyl-1,4-benzoquinone
50 - 289
methyl-beta-D-glucoside
0.03 - 4.8
O2
0.0832 - 0.65
oxygen
902
Sucrose
-
at pH 6.5 and 25C
0.09
tetrabromo-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.088
tetrachloro-1,4-benzoquinone
-
pH 6.5, substrate: D-glucose
0.22 - 9.42
tetrafluoro-1,4-benzoquinone
192
trehalose
-
pH 7.0, 37C
additional information
additional information
-