Information on EC 1.1.2.B5 - PQQ-dependent quinohemoprotein pyranose dehydrogenase

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The expected taxonomic range for this enzyme is: Coprinopsis cinerea

EC NUMBER
COMMENTARY hide
1.1.2.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
PQQ-dependent quinohemoprotein pyranose dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a D-pyranose + a ferricytochrome c = a dehydro-D-pyranose + a ferrocytochrome c + 2 H+
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
D-pyranose:cytochrome-c oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-arabinose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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47.5% activity compared to D-glucosone
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?
D-glucosone + ferricytochrome c
? + ferrocytochrome c
show the reaction diagram
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100% activity
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?
D-lyxose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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13.4% activity compared to D-glucosone
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?
D-talose + ferricytochrome c
? + ferrocytochrome c
show the reaction diagram
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24.8% activity compared to D-glucosone
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?
L-fucose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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77.7% activity compared to D-glucosone
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?
L-fucose + ferricytochrome c
2-dehydro-L-fucose + ferrocytochrome c
show the reaction diagram
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-
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?
L-fucose + phenazine methosulfate
2-dehydro-L-fucose + reduced phenazine methosulfate
show the reaction diagram
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?
L-galactose + ferricytochrome c
? + ferrocytochrome c
show the reaction diagram
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65.4% activity compared to D-glucosone
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?
L-glucose + ferricytochrome c
? + ferrocytochrome c
show the reaction diagram
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9.2% activity compared to D-glucosone
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?
L-gulose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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52.1% activity compared to D-glucosone
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?
L-lyxose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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4.0% activity compared to D-glucosone
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?
L-mannose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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3.6% activity compared to D-glucosone
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?
L-rhamnose + 2,6-dichlorophenolindophenol
? + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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0.7% activity compared to D-glucosone
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?
L-xylose + ferricytochrome c
? + ferrocytochrome c
show the reaction diagram
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11.9% activity compared to D-glucosone
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?
additional information
?
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the enzyme prefers monosaccharides with equatorial C-2, C-3 hydroxyl groups and an axial C-4 hydroxyl group in the 1C4 chair conformation
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyrroloquinoline quinone
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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1 mM Ca2+ is used in assay conditions
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30.3
D-arabinose
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at pH 8.5 and 30°C
7.9
D-glucosone
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at pH 8.5 and 30°C
66.8
D-Lyxose
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at pH 8.5 and 30°C
23.1
D-talose
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at pH 8.5 and 30°C
3.3 - 6.1
ferricytochrome c
24.8
L-fucose
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at pH 8.5 and 30°C
49.7
L-galactose
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at pH 8.5 and 30°C
363
L-Glucose
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at pH 8.5 and 30°C
84.7
L-gulose
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at pH 8.5 and 30°C
236
L-Xylose
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at pH 8.5 and 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
35.5
D-arabinose
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at pH 8.5 and 30°C
74.1
D-glucosone
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at pH 8.5 and 30°C
12.9
D-Lyxose
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at pH 8.5 and 30°C
17.8
D-talose
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at pH 8.5 and 30°C
30.7 - 53.5
ferricytochrome c
56.4
L-fucose
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at pH 8.5 and 30°C
56.1
L-galactose
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at pH 8.5 and 30°C
24.9
L-Glucose
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at pH 8.5 and 30°C
53.3
L-gulose
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at pH 8.5 and 30°C
22.4
L-Xylose
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at pH 8.5 and 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.17
D-arabinose
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at pH 8.5 and 30°C
9.37
D-glucosone
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at pH 8.5 and 30°C
0.19
D-Lyxose
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at pH 8.5 and 30°C
0.77
D-talose
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at pH 8.5 and 30°C
2.27
L-fucose
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at pH 8.5 and 30°C
1.13
L-galactose
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at pH 8.5 and 30°C
0.07
L-Glucose
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at pH 8.5 and 30°C
0.63
L-gulose
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at pH 8.5 and 30°C
0.09
L-Xylose
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at pH 8.5 and 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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for reduction of phenazine methosulfate
8.5
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for reduction of ferricytochrome c
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris
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