Information on EC 1.1.2.B3 - quinoprotein methanol dehydrogenase (cytochrome c559)

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The expected taxonomic range for this enzyme is: Pseudomonas

EC NUMBER
COMMENTARY hide
1.1.2.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
quinoprotein methanol dehydrogenase (cytochrome c559)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alcohol + 2 cytochrome c550 = aldehyde + 2 reduced cytochrome c550 + 2 H+
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
methanol:ferricytochrome c550 oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-propanediol + acceptor
? + reduced acceptor
show the reaction diagram
1-butanol + acceptor
butanal + reduced acceptor
show the reaction diagram
1-propanol + acceptor
propanal + reduced acceptor
show the reaction diagram
2-propanol + acceptor
2-propanone + reduced acceptor
show the reaction diagram
artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol
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-
?
alcohol + cytochrome c550
aldehyde + reduced cytochrome c550
show the reaction diagram
ethanol + acceptor
acetaldehyde + reduced acceptor
show the reaction diagram
ethanol + ferricytochrome c550
acetaldehyde + ferrocytochrome c550
show the reaction diagram
phenylethanol + ferricytochrome c
phenylacetaldehyde + ferrocytochrome c
show the reaction diagram
PedH catalyzes a step in the aerobic transformation of 2-phenylethylamine and 2-phenylethanol. PedH does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alcohol + cytochrome c550
aldehyde + reduced cytochrome c550
show the reaction diagram
ethanol + ferricytochrome c550
acetaldehyde + ferrocytochrome c550
show the reaction diagram
Q9Z4J7
regulation of ethanol-oxidation system in Pseudomonas aeruginosa
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?
phenylethanol + ferricytochrome c
phenylacetaldehyde + ferrocytochrome c
show the reaction diagram
B1N7J0, B1N7J5
PedH catalyzes a step in the aerobic transformation of 2-phenylethylamine and 2-phenylethanol. PedH does not belong to the class of PQQ-dependent alcohol dehydrogenases containing a c-type cytochrome heme domain, but to others that transfer electrons to a separate soluble c-type cytochrome
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyrroloquinoline quinone
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Amine
the enzymatic activity of the mutant enzyme in the artificial dye test with N-methylphenazonium methyl sulfate (PMS) and 2,6-dichlorophenol indophenol (DCPIP) at pH 9 does not depend on an activating amine which is essential for wild type activity under these condition
ethylamine
5 mM, wild-type enzyme shows strict dependence on an activator
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21 - 266
1,3-Propanediol
0.43 - 12
1-butanol
0.062 - 3.8
1-propanol
1.4 - 1.5
2-propanol
0.012 - 12
ethanol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11
substrate: 2-propanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, wild-type enzyme; substrate: ethanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, wild-type enzyme
15
substrate: 1-propanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, wild-type enzyme
20
substrate: ethanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, mutant enzyme C105A/C106A
40
substrate: 1,3-propanediol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, wild-type enzyme
42
substrate: 1-butanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, wild-type enzyme
52
substrate: 1-propanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, mutant enzyme C105A/C106A
94
substrate: 2-propanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, mutant enzyme C105A/C106A
224
substrate: 1,3-propanediol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, mutant enzyme C105A/C106A
461
substrate: 1-butanol, pH 9 in the artificial dye test at fixed concentrations of N-methylphenazonium methyl sulfate and 2,6-dichlorophenol indophenol in the presence of 5 mM ethylamine, mutant enzyme C105A/C106A
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64870
x * 64870, PedH, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64870, PedH, sequence calculation
additional information
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
exaAB, DNA and amino acid sequence determination and analysis, exaA encodes the enzyme, while exaB encodes a cytochrome 550
gene pedE, DNA and amino acid sequence determination and analysis; gene pedH, DNA and amino acid sequence determination and analysis
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C105A/C106A
additional information