Information on EC 1.1.2.2 - mannitol dehydrogenase (cytochrome)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.2.2
-
RECOMMENDED NAME
GeneOntology No.
mannitol dehydrogenase (cytochrome)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-mannitol + a ferricytochrome c = D-fructose + a ferrocytochrome c + 2 H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pentose and glucuronate interconversions
-
-
Fructose and mannose metabolism
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SYSTEMATIC NAME
IUBMB Comments
D-mannitol:cytochrome-c 2-oxidoreductase
The enzyme from the bacterium Gluconobacter oxydans acts on polyols with a D-lyxo configuration, such as D-mannitol and D-sorbitol, with preference towards the former.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-78-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyclic D-erythro polyols + ferricytochrome c
acyclic D-erythro ketoses + ferrocytochrome c
show the reaction diagram
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
D-sorbitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannitol + ferricytochrome c
D-fructose + ferrocytochrome c
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c
methylene blue
-
suitable as electron acceptor
phenazine methosulfate
-
suitable as electron acceptor
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
restores activity after inactivation with EDTA and storage
Mg2+
-
restores activity after inactivation with EDTA and storage
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
-
strong inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17
D-arabitol
-
-
0.098 - 34
D-mannitol
20
D-sorbitol
-
-
44
ribitol
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
p-quinone
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20000
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
51000
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
69000
-
alpha,beta,gamma 1 * 69000 + 1 * 51000 + 1 * 20000, SDS-PAGE
140000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
-
stable for 30 min at 30°C
389622
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
stable up to 50°C at pH 6
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, stable for several weeks, lost activity can be restored by adding Ca2+ or Mg2+
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE