Information on EC 1.1.1.B65 - (R)-mandelate dehydrogenase [NAD(P)+]

for references in articles please use BRENDA:EC1.1.1.B65
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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.1.1.B65
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
(R)-mandelate dehydrogenase [NAD(P)+]
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SYSTEMATIC NAME
IUBMB Comments
(R)-mandelate:NAD(P)+ 2-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)
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Manually annotated by BRENDA team
isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-mandelate + NAD+
phenylglyoxylate + NADH + H+
show the reaction diagram
(R)-mandelate + NADP+
phenylglyoxylate + NADPH + H+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-mandelate + NAD+
phenylglyoxylate + NADH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
activates 44% at 1 mM and 69% at 10 mM
Mn2+
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21% activation at 1 mM, and 61% inhibition at 10 mM
Na+
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activates 12% at 10 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
inhibits 4% aT 1 mM and 33% at 10 mM
EDTA
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inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively
Hg2+
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strong inhibition at 1 mM
Li+
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inhibits 17% at 10 mM
Mg2+
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inhibits 19% at 1 mM and 31% at 10 mM
Mn2+
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21% activation at 1 mM, and 61% inhibition at 10 mM
Ni2+
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inhibits 39% at 1 mM and 63% at 10 mM
SDS
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strongly inhibits the enzyme activity at 0.25-0.75%
Triton X-100
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0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity
Zn2+
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strong inhibition at 10 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
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enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM
Triton X-100
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0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
(R)-mandelate
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pH 8.5, 30C
0.018
NAD+
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pH 8.5, 30C
0.015
NADP+
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pH 8.5, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
(R)-mandelate
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pH 8.5, 30C
0.9
NAD+
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pH 8.5, 30C
0.3
NADP+
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pH 8.5, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03
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crude enzyme extract, pH 8.5, 30C
0.33
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purified native enzyme, pH 8.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
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activity range, profile overview
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 55
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activity range, profile overview
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30C. After 96 h at 4C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5
743100
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography
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