Information on EC 1.1.1.B47 - succinate semialdehyde reductase (NADPH)

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The expected taxonomic range for this enzyme is: Metallosphaera sedula

EC NUMBER
COMMENTARY hide
1.1.1.B47
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
succinate semialdehyde reductase (NADPH)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-hydroxybutanoate + NADP+ = succinate semialdehyde + NADPH + H+
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
CO2 fixation in Crenarchaeota
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-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxybutanoate:NADP+ oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
succinate semialdehyde + NADH + H+
4-hydroxybutanoate + NAD+
show the reaction diagram
succinate semialdehyde + NADPH + H+
4-hydroxybutanoate + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
succinate semialdehyde + NADPH + H+
4-hydroxybutanoate + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
the enzyme contains 1.9 mol of zinc per mol of enzyme monomer
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
NADH
65°C, pH 7.9
0.006
NADPH
65°C, pH 7.9
0.052
succinate semialdehyde
65°C, pH 7.9, cosubstrate: NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
440
NADPH
Metallosphaera sedula
A4YGN0
65°C, pH 7.9
440
succinate semialdehyde
Metallosphaera sedula
A4YGN0
65°C, pH 7.9
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.15
cell extract, NADH-dependent reaction, 65°C, pH 7.9
1.4
cell extract, NADPH-dependent reaction, 65°C, pH 7.9
700
purified enzyme, NADPH-dependent reaction, 65°C, pH 7.9
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
at 65°C, half-maximal activity at pH 6.5 and at pH 8.0
6.9 - 8.4
at 20°C, half-maximal activity at pH 6.9 and at pH 8.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
2 * 40000, SDS-PAGE
74000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli Rosetta 2(DE3)