Information on EC 1.1.1.B35 - D-arabinose 1-dehydrogenase (NADP+)

for references in articles please use BRENDA:EC1.1.1.B35
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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.B35
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
D-arabinose 1-dehydrogenase (NADP+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-arabinose + NADP+ = D-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
degradation of pentoses
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SYSTEMATIC NAME
IUBMB Comments
D-arabinose:NADP+ 1-oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R/S)-acetoin + NADPH
(2S,3S)-2,3-butanediol + NADP+
show the reaction diagram
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Ara1p is as an oxidoreductase that can reduce racemic acetoin to meso-2,3-butanediol and (2S,3S)-2,3-butanediol in the presence of NADPH
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-
?
D-arabinose + NAD(P)+
D-arabino-1,4-lactone + NAD(P)H
show the reaction diagram
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-
-
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?
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH
show the reaction diagram
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-
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ir
D-arabinose + NAD+
D-arabinono-1,4-lactone + NADH + H+
show the reaction diagram
D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH
show the reaction diagram
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-
-
-
?
D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
D-ribose + NADP+
D-ribonolactone + NADPH
show the reaction diagram
D-xylose + NADP+
D-xylonolactone + NADPH + H+
show the reaction diagram
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-
-
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?
L-fucose + NADP+
6-deoxy-L-galactono-1,4-lactone + NADPH + H+
show the reaction diagram
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-
-
?
L-fucose + NADP+
L-fuconolactone + NADPH
show the reaction diagram
L-fucose + NADP+
L-fuconolactone + NADPH + H+
show the reaction diagram
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with NADP+
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-
?
L-galactose + NADP+
L-galactono-1,4-lactone + NADPH + H+
show the reaction diagram
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-
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?
L-galactose + NADP+
L-galactono-1,5-lactone + NADPH
show the reaction diagram
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-
-
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?
L-galactose + NADP+
L-galactonolactone + NADPH
show the reaction diagram
L-xylose + NADP+
L-xylono-1,4-lactone + NADPH
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-arabinose + NAD(P)+
D-arabino-1,4-lactone + NAD(P)H
show the reaction diagram
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-
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?
D-arabinose + NADP+
D-arabinono-1,4-lactone + NADPH + H+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
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the catalytic efficiency of NADP+ in combination with D-arabinose is 36-fold higher than that of NAD+
NADP+
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4 - 161
D-arabinose
0.22 - 98
L-fucose
180
L-galactose
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1.25
NAD+
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pH 7.5, 70°C, determined with 10 mM D-arabinose
0.038 - 0.04
NADP+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23.8
D-arabinose
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pH 7.5, 80°C
17.7
D-ribose
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pH 7.5, 80°C
26.8
L-fucose
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pH 7.5, 80°C
17.7
L-galactose
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pH 7.5, 80°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.6
D-arabinose
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pH 7.5, 70°C, determined with 0.4 mM NADP+
67
L-fucose
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pH 7.5, 70°C, determined with 0.4 mM NADP+
14.5
NAD+
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pH 7.5, 70°C, determined with 10 mM D-arabinose
525
NADP+
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pH 7.5, 70°C, determined with 10 mM D-arabinose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 9.1
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pH 7.1: about 40% of maximal activity, pH 9.1: about 60% of maximal activity, pH 9.5: about 30% of maximal activity
7.3 - 9.3
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more than 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 100
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70°C: about 40% of maximal activity, 100°C: about 90% of maximal activity
74 - 100
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more than 50% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37291
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4 * 37291, calculated from sequence
39000
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1 * 39000 + 1 * 40000, SDS-PAGE
40000
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1 * 39000 + 1 * 40000, SDS-PAGE; 1 * 40000 + 1 + 39000, SDS-PAGE
74000
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gel filtration
149700
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mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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1 * 39000 + 1 * 40000, SDS-PAGE
heterodimer
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1 * 40000 + 1 + 39000, SDS-PAGE
homotetramer
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure at 2 A resolution
the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85
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half-life: 42 min
90
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half-life: 26 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial; using Ni-NTA chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
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expression in Escherichia coli
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