Information on EC 1.1.1.B19 - xylitol dehydrogenase (NAD+)

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The expected taxonomic range for this enzyme is: Aeribacillus pallidus

EC NUMBER
COMMENTARY hide
1.1.1.B19
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
xylitol dehydrogenase (NAD+)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
xylitol + NAD+ = L-xylulose + NADH + H+
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
xylitol:NAD+ 2-oxidoreductase (L-xylulose-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-arabitol + NADH + H+
D-ribulose + NAD+
show the reaction diagram
15% conversion in resting cell reaction
-
-
?
D-iditol + NAD+
D-sorbose + NADH + H+
show the reaction diagram
72% conversion in resting cell reaction
-
-
?
D-talitol + NADH + H+
D-tagatose + NAD+
show the reaction diagram
15% conversion in resting cell reaction
-
-
?
D-threitol + NAD+
erythrulose + NADH + H+
show the reaction diagram
48% conversion in resting cell reaction
-
-
?
D-xylulose + NADH + H+
xylitol + NAD+
show the reaction diagram
-
-
-
?
ribitol + NADH + H+
D-ribulose + NAD+
show the reaction diagram
15% conversion in resting cell reaction
-
-
?
xylitol + NAD+
L-xylulose + NADH + H+
show the reaction diagram
50% conversion in resting cell reaction
-
-
r
additional information
?
-
no substrate: DL-rhamnitol, galactitol, allitol, DL-fucitol, L-iditol, L-threitol, DL-sorbitol, DL-mannitol, erythritol
-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
specific for NAD+
additional information
no activity with NADP+
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
1 mM, 50.8% of the activity with Mn2+
Mg2+
1 mM, 36.9% of the activity with Mn2+
additional information
no significant stimulation by Na+, Cu2+, K+, Zn2+, Co2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.64
D-arabitol
pH 10.0, 40C
0.83
D-threitol
pH 10.0, 40C
1.27
L-xylulose
pH 10.0, 40C
0.034
NAD+
pH 10.0, 40C
2.06
xylitol
pH 10.0, 40C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.695
D-arabitol
Aeribacillus pallidus
E0D583
pH 10.0, 40C
2.75
D-threitol
Aeribacillus pallidus
E0D583
pH 10.0, 40C
1.11
L-xylulose
Aeribacillus pallidus
E0D583
pH 10.0, 40C
2.75
xylitol
Aeribacillus pallidus
E0D583
pH 10.0, 40C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26
D-arabitol
Aeribacillus pallidus
E0D583
pH 10.0, 40C
1786
3.31
D-threitol
Aeribacillus pallidus
E0D583
pH 10.0, 40C
3460
0.87
L-xylulose
Aeribacillus pallidus
E0D583
pH 10.0, 40C
1238
1.33
xylitol
Aeribacillus pallidus
E0D583
pH 10.0, 40C
416
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 27333, calculated, x * 28000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
1 h, 66% residual acitivity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
production of L-xylulose from xylitol using a resting cell reaction leads to 35% L-xylulose within 24 h, starting from 5% xylitol as initial concentration