Information on EC 1.1.1.B18 - L-1-amino-2-propanol dehydrogenase

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The expected taxonomic range for this enzyme is: Rhodococcus erythropolis

EC NUMBER
COMMENTARY hide
1.1.1.B18
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
L-1-amino-2-propanol dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ = (2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
reduction
SYSTEMATIC NAME
IUBMB Comments
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol:NADP+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
1262091-13-3
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1262091-13-3
UniProt-ID D6MMN3
283594-89-8
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283594-89-9
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
show the reaction diagram
DL-1-amino-2-butanol + NADPH + H+
? + NADP+
show the reaction diagram
DL-2-aminocyclohexanol + NADPH + H+
? + NADP+
show the reaction diagram
L-1-amino-2-propanol + NADPH + H+
? + NADP+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol
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product inhibition above 20 mg/ml
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.43
-
pH 7.5, 43C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
105000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene aadh, DNA and amino acid sequence determination and analysis, expression analysis
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced on cultivation with aminoalcohols such as 1-amino-2-propanol, 1-amino-2-butanol and 2-aminocyclohexanol
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expression is induced on cultivation with aminoalcohols such as 1-amino-2-propanol, 1-amino-2-butanol and 2-aminocyclohexanol; the enzyme is induced by the addition of several aminoalcohols, such as 1-amino-2-propanol
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OrfE is a negative regulator that represses gene aadh expression in the absence of 1-amino-2-propanol
the enzyme is induced by the addition of several aminoalcohols, such as 1-amino-2-propanol
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis