Information on EC 1.1.1.97 - 3-hydroxybenzyl-alcohol dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.97
-
RECOMMENDED NAME
GeneOntology No.
3-hydroxybenzyl-alcohol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxybenzyl alcohol + NADP+ = 3-hydroxybenzaldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
patulin biosynthesis
-
-
Toluene degradation
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
3-hydroxybenzyl-alcohol:NADP+ oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-73-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzyl alcohol + NADP+
3-hydroxybenzaldehyde + NADPH
show the reaction diagram
3-methoxybenzyl alcohol + NADP+
3-methoxybenzaldehyde + NADPH
show the reaction diagram
4-hydroxybenzyl alcohol + NADP+
4-hydroxybenzaldehyde + NADPH
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
-
29% activity compared to 3-hydroxybenzyl alcohol
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzyl alcohol + NADP+
3-hydroxybenzaldehyde + NADPH
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diethyldicarbonate
iodoacetic acid
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
1.0 mM and 10 mM, 56% increase in activity, enzyme activity in cell extracts
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
3-Hydroxybenzyl alcohol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.57
-
enzyme activity in crude extracts from cells grown on 3,5-xylenol
0.65
-
enzyme activity in crude extracts from cells grown on 3-hydroxy-5-methylbenzoate
0.7
-
enzyme activity in crude extracts from cells grown on gentisate
0.78
-
enzyme activity in crude extracts from cells grown on m-hxdroxybenzoate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
sedimentation equilibrium
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 36000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
14 min half-life of crude enzyme at 30°C in 100 mM Tris-HCl, pH 7.6, half-life is increased to 77 and 80 min by 2 mM MnCl2 and FeCl3 respectively, 3 mM NADPH increase half-life to 146 min
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Bio-Gel A, 2',5'-ADP-agarose, DEAE-Sephacell, 2',5'-ADP-agarose
-
streptomycin sulfate, ammonium sulfate, Sephadex G-200, 6.55fold purification
-