Information on EC 1.1.1.96 - diiodophenylpyruvate reductase

Word Map on EC 1.1.1.96
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.96
-
RECOMMENDED NAME
GeneOntology No.
diiodophenylpyruvate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+ = 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-(3,5-diiodo-4-hydroxyphenyl)lactate:NAD+ oxidoreductase
Substrates contain an aromatic ring with a pyruvate side chain. The most active substrates are halogenated derivatives. Compounds with hydroxy or amino groups in the 3 or 5 position are inactive.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-31-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,5-dinitro-4-hydroxyphenylpyruvate + NADH
3,5-dinitro-4-hydroxyphenyllactate + NAD+
show the reaction diagram
-
-
-
r
3-(3,5-dibromo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-dibromo-4-hydroxyphenyl)lactate + NAD+
show the reaction diagram
-
-
-
r
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
show the reaction diagram
3-bromo-4-hydroxyphenylpyruvate + NADH
3-bromo-4-hydroxyphenyllactate + NAD+
show the reaction diagram
-
-
-
r
3-fluorophenylpyruvate + NADH
3-fluorophenyllactate + NAD+
show the reaction diagram
-
-
-
r
4-hydroxyphenylpyruvate + NADH
4-hydroxyphenyllactate + NAD+
show the reaction diagram
indolepyruvate + NADH
indolelactate + NAD+
show the reaction diagram
oxaloacetate + NADH
malate + NAD+
show the reaction diagram
-
order of activity, oxaloacetate, 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate, indolepyruvate, phenylpyruvate
-
r
phenylpyruvate + NADH
phenyllactate + NAD+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
15% activity compared to NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,5-dihydroxybenzoic acid
2,5-dihydroxyphenyllactic acid
-
1 mM, 20% inhibition
-
3,5-Diiodo-4-hydroxyphenyllactic acid
-
1 mM, 18% inhibition
3,5-diiodotyrosine
-
1 mM, 15% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 0.043
3,5-diiodo-4-hydroxyphenylpyruvate
1.9
4-hydroxyphenylpyruvate
-
-
0.2
Indolepyruvate
-
-
0.014 - 0.052
NADH
0.14
oxaloacetate
-
-
1.6 - 4.8
phenylpyruvate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41.75
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
-
sucrose-density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 40000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62
-
not completely inactivated after 2 min, completely inactivated above 72C
72
-
complete inactivation in 2 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50 mM potassium phosphate, pH 7.5, 2 months, no loss of activity
-
4C, 50 mM potassium phosphate, pH 7.5, 2 weeks, no loss of activity
-
5C, 160 mM phosphate, pH 6.5, 1 month, 20% activity lost
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, calcium phosphate gel
-
DEAE-cellulose, hydroxyapatite, isoelectric focusing, Sephadex G-150
-