Information on EC 1.1.1.92 - oxaloglycolate reductase (decarboxylating)

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.1.1.92
-
RECOMMENDED NAME
GeneOntology No.
oxaloglycolate reductase (decarboxylating)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glycerate + NAD(P)+ + CO2 = 2-hydroxy-3-oxosuccinate + NAD(P)H + 2 H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glyoxylate and dicarboxylate metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
D-glycerate:NAD(P)+ oxidoreductase (carboxylating)
Also reduces hydroxypyruvate to D-glycerate and glyoxylate to glycolate.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-28-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-dihydroxysuccinate + NAD(P)H
D-glycerate + NAD(P)+ + CO2
show the reaction diagram
-
-
-
ir
2-hydroxy-3-oxosuccinate + NAD(P)H
D-glycerate + NAD(P)+ + CO2
show the reaction diagram
glyoxylate + NAD(P)H
glycolic acid + NAD(P)+
show the reaction diagram
-
-
-
ir
hydroxypyruvate + NAD(P)H
D-glycerate + NAD(P)+
show the reaction diagram
-
-
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-hydroxy-3-oxosuccinate + NAD(P)H
D-glycerate + NAD(P)+ + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 2.5
2,3-dihydroxysuccinate
3.3 - 8
glyoxylate
1 - 2.5
Hydroxypyruvate
0.14 - 0.17
NADH
0.015 - 0.024
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.6
-
substrate 2,3-dihydroxy-succinate
7.7
-
substrate hydroxypyruvate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
63000
-
sedimentation equilibrium
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 40 mM Tris-HCl, 30% glycerol, 5 mM mercaptoethanol, 6 months, 10% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-100, disc electrophoresis, Sephadex G-100, crystallization
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