Information on EC 1.1.1.91 - aryl-alcohol dehydrogenase (NADP+)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.91
-
RECOMMENDED NAME
GeneOntology No.
aryl-alcohol dehydrogenase (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aromatic alcohol + NADP+ = an aromatic aldehyde + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-alcohol:NADP+ oxidoreductase
Also acts on some aliphatic aldehydes, but cinnamaldehyde was the best substrate found.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-27-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
-
Manually annotated by BRENDA team
wheatgrass
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
American Type Culture Collection No.607
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
JCM3016
-
-
Manually annotated by BRENDA team
JCM3016
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
rye
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
tomato
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Triticale turgidocereale
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E)-oct-2-enal + NADPH + H+
trans-2-octen-1-ol + NADP+
show the reaction diagram
-
-
-
?
(R)-1-phenylethanol + NAD+
1-phenylacetaldehyde + NADH + H+
show the reaction diagram
(R)-2-octanol + NADPH + H+
?
show the reaction diagram
(S)-1-phenylethanol + NADP+
1-phenylacetaldehyde + NADPH + H+
show the reaction diagram
(S)-2-octanol + NADPH + H+
?
show the reaction diagram
1-butanol + NADP+
butyraldehyde + NADPH + H+
show the reaction diagram
56% activity compared to crotyl alcohol
-
-
?
1-propanol + NADP+
propionaldehyde + NADPH + H+
show the reaction diagram
17.4% activity compared to crotyl alcohol
-
-
?
2,4-dimethoxybenzyl alcohol + NADP+
2,4-dimethoxybenzyl aldehyde + NADPH + H+
show the reaction diagram
-
-
-
?
2-fluorobenzaldehyde + NADPH
2-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
2-hydroxyacetophenone + NADPH + H+
(S)-1,2-phenylethanediol + NADP+
show the reaction diagram
-
-
-
?
2-methoxybenzaldehyde + NADPH
2-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
2-methyl-propanol + NADPH + H+
?
show the reaction diagram
2-nitrobenzaldehyde + NADPH
2-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3,4,5-trimethoxybenzaldehyde + NADPH
3,4,5-trimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NADPH
3,4-dihydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3,4-dimethoxybenzaldehyde + NADH
3,4-dimethoxybenzyl alcohol + NAD+
show the reaction diagram
-
-
-
-
r
3,4-dimethoxybenzaldehyde + NADPH
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
3,4-dimethoxybenzyl alcohol + NADP+
?
show the reaction diagram
-
-
-
?
3,4-dimethoxybenzyl alcohol + NADP+
veratraldehyde + NADPH + H+
show the reaction diagram
-
-
-
r
3,5-dimethoxybenzaldehyde + NADPH
3,5-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
3-chlorobenzaldehyde + NADPH
3-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-fluorobenzaldehyde + NADPH
3-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-hydroxy-4-methoxy-benzaldehyde + NADPH
3-hydroxy-4-methoxy-benzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-hydroxy-4-methoxybenzaldehyde + NADPH + H+
3-hydroxy-4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
3-methoxybenzaldehyde + NADPH
3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
3-methyl-butanol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
3-nitrobenzaldehyde + NADPH
3-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-(hydroxymethyl)benzoic acid + NAD+
? + NADH + H+
show the reaction diagram
-
-
-
?
4-benzyloxy-3-methoxybenzaldehyde + NADPH + H+
4-benzyloxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NADPH
4-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-fluorobenzaldehyde + NADPH
4-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
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r
4-hydroxy-3-methoxy-benzaldehyde + NADPH
4-hydroxy-3-methoxy-benzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxy-3-methoxybenzaldehyde + NADPH + H+
4-hydroxy-3-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NADPH
4-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-hydroxybenzaldehyde + NADPH + H+
4-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NADPH
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
4-methoxybenzaldehyde + NADPH + H+
4-methoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
?
4-methoxybenzyl alcohol + NADP+
4-methoxybenzaldehyde + NADPH
show the reaction diagram
4-nitrobenzaldehyde + NADPH
4-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
5-(hydroxymethyl)-2-furaldehyde + NADPH + H+
(furan-2,5-diyl)dimethanol + NADP+
show the reaction diagram
-
relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
-
-
-
-
r
acetophenone + NADPH + H+
?
show the reaction diagram
-
-
-
r
alpha-ionone + NADPH + H+
?
show the reaction diagram
-
-
-
r
benzaldehyde + NADPH
benzyl alcohol + NADP+
show the reaction diagram
benzaldehyde + NADPH + H+
benzyl alcohol + NAD+
show the reaction diagram
reduction of benzaldehyde at pH 6.0 is NADPH-dependent while NADH does not yield detectable activity under the same conditions
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-
r
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
show the reaction diagram
highest reductive activity with benzaldehyde
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r
benzil + NADPH + H+
benzoin + NADP+
show the reaction diagram
-
-
-
-
?
benzyl alcohol + NAD+
benzaldehyde + NADPH + H+
show the reaction diagram
oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent while NADP+ did not yield detectable activity under the same conditions
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-
r
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
butanol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
cinnamic alcohol + NADP+
cinnamic aldehyde + NADPH
show the reaction diagram
coniferyl alcohol + NADP+
coniferylaldehyde + NADPH + H+
show the reaction diagram
crotonaldehyde + NADPH + H+
crotonyl alcohol + NADP+
show the reaction diagram
-
-
-
r
crotonyl alcohol + NADP+
crotonaldehyde + NADPH + H+
show the reaction diagram
highest oxidative activity with crotyl alcohol
-
-
r
decanal + NADPH
decanol + NADP+
show the reaction diagram
-
relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
heptaldehyde + NADPH
?
show the reaction diagram
-
relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
hexanal + NADPH
hexanol + NADP+
show the reaction diagram
-
relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
m-bromobenzaldehyde + NADPH + H+
m-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-chlorobenzaldehyde + NADPH + H+
m-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-fluorobenzaldehyde + NADPH + H+
m-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-hydroxybenzaldehyde + NADPH + H+
m-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
m-nitrobenzaldehyde + NADPH
m-nitrobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
n-butanol + NADP+
n-butanal + NADPH
show the reaction diagram
-
-
-
-
r
n-pentanol + NADP+
n-pentanal + NADPH
show the reaction diagram
-
-
-
-
r
n-propanol + NADP+
n-propanal + NADPH
show the reaction diagram
-
-
-
-
r
nonanal + NADPH
nonanol + NADP+
show the reaction diagram
-
relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
o-bromobenzaldehyde + NADPH + H+
o-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
o-chlorobenzaldehyde + NADPH + H+
o-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
o-fluorobenzaldehyde + NADPH + H+
o-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
octanal + NADPH
octanol + NADP+
show the reaction diagram
-
relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
p-aminobenzyl alcohol + NADP+
p-aminobenzaldehyd + NADPH
show the reaction diagram
-
-
-
-
r
p-bromobenzaldehyde + NADPH + H+
p-bromobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-chlorobenzaldehyde + NADPH + H+
p-chlorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-fluorobenzaldehyde + NADPH + H+
p-fluorobenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-hydroxybenzaldehyde + NADPH + H+
p-hydroxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
p-methylbenzaldehyde + NADPH
p-methylbenzyl alcohol + NADP+
show the reaction diagram
-
-
-
-
r
pentanal + NADPH
pentanol + NADP+
show the reaction diagram
-
relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
phenylacetaldehyde + NADPH
phenylethanol + NADP+
show the reaction diagram
-
relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
phenylacetaldehyde + NADPH + H+
phenylethanol + NADP+
show the reaction diagram
-
-
-
-
?
rac-4-methyl-2-pentanol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
rac-butan-2,3-diol + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
salicyl alcohol + NADP+
p-hydroxybenzaldehyde + NADPH
show the reaction diagram
-
i.e. p-hydroxybenzyl alcohol
-
-
r
trans-4-phenyl-3-buten-2-one + NADPH + H+
?
show the reaction diagram
-
-
-
r
trans-cinnamaldehyde + NADPH
?
show the reaction diagram
-
relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde)
-
-
r
vanillin + NADPH + H+
vanillic alcohol + NADP+
show the reaction diagram
-
-
-
r
veratraldehyde + NADPH + H+
3,4-dimethoxybenzyl alcohol + NADP+
show the reaction diagram
-
-
-
r
veratryl alcohol + NADP+
veratraldehyde + NADPH
show the reaction diagram
-
i.e. 3,4-dimethoxybenzyl alcohol
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
113% activity at 1 mM
Zn2+
dependent on
additional information
Ca2+, Na+, Mg2+, Mn2+, and K+ have no significant effect on the enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
3,4-dimethoxybenzaldehyde
-
-
3,4-dimethoxybenzyl alcohol
-
-
3,5-dimethoxybenzaldehyde
-
-
3-hydroxy-4-methoxy-benzaldehyde
-
-
4-hydroxy-3-methoxybenzaldehyde
-
-
5,5'-dithio-bis(2-nitrobenzoic acid)
-
-
5-(hydroxymethyl)-2-furaldehyde
-
-
Ag+
complete inhibition at 1 mM
AgNO3
Al3+
13.5% inhibition at 1 mM
Co2+
80.5% inhibition at 1 mM
Cu2+
complete inhibition at 1 mM
diphenyl hydantoin
-
-
Fe2+
39.7% inhibition at 1 mM
iodoacetate
94.5% residual activity at 10 mM
Iodosobenzoate
-
-
p-chloromercuribenzoate
trans-cinnamaldehyde
-
-
Zn2+
99.5% inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetone
125% activity at 20% (v/v) acetone
dithiothreitol
123% activity at 1 mM
EDTA
110% activity at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.5
(R)-1-phenylethanol
wild-type, pH 8.3, 30°C
1.2 - 17.6
(S)-1-phenylethanol
7.1 - 7.7
(S)-2-octanol
0.012
3,4-dimethoxybenzaldehyde
-
pH 6.1, 30°C
0.37
3,4-dimethoxybenzyl alcohol
-
pH 6.1, 30°C
0.022
3,5-dimethoxybenzaldehyde
-
pH 6.1, 30°C
1.4
3-hydroxy-4-methoxy-benzaldehyde
-
pH 6.1, 30°C
0.55
3-phenylpropionaldehyde
-
-
1.4
4-Hydroxy-3-methoxy-benzaldehyde
-
pH 6.1, 30°C
25
4-hydroxy-3-methoxy-benzyl alcohol
-
pH 6.1, 30°C
0.09 - 0.5
4-methoxybenzaldehyde
1.7
4-methoxybenzyl alcohol
-
-
0.27
5-(hydroxymethyl)-2-furaldehyde
-
pH 6.1, 30°C
1.7
acetaldehyde
-
-
0.34 - 1.7
benzaldehyde
0.057 - 14
benzyl alcohol
0.22
cinnamic aldehyde
-
-
0.55
coniferyl alcohol
-
-
0.14
coniferyl aldehyde
-
-
3.3
crotonaldehyde
at pH 8.0 and 55°C
9.1
crotonyl alcohol
at pH 8.0 and 55°C
0.16
m-bromobenzaldehyde
-
-
0.1
m-chlorobenzaldehyde
-
-
0.42
m-fluorobenzaldehyde
-
-
1.1
m-hydroxybenzaldehyde
-
-
0.21
m-nitrobenzaldehyde
-
-
0.8 - 3.1
NAD+
0.0026 - 0.22
NADH
0.024 - 2.3
NADP+
0.017 - 0.1
NADPH
0.25
o-bromobenzaldehyde
-
-
0.2
o-chlorobenzaldehyde
-
-
0.27
o-fluorobenzaldehyde
-
-
0.05
o-nitrobenzaldehyde
-
-
0.04
p-aminobenzyl alcohol
-
-
0.22
p-bromobenzaldehyde
-
-
0.34
p-chlorobenzaldehyde
-
-
0.26
p-fluorobenzaldehyde
-
-
0.09
p-Hydroxybenzaldehyde
-
-
0.3
p-methylbenzaldehyde
-
-
0.04
p-nitrobenzaldehyde
-
-
0.53
phenylacetaldehyde
-
pH 6.1, 30°C
3.4
trans-cinnamaldehyde
-
pH 6.1, 30°C
0.25
veratraldehyde
at pH 6.0 temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0047
(R)-1-phenylethanol
Ralstonia sp.
C0IR58
wild-type, pH 8.3, 30°C
0.0115 - 0.783
(S)-1-phenylethanol
4.3 - 13.7
(S)-2-octanol
8.833
3,4-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
2.55
3,4-dimethoxybenzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30°C
9.83
3,5-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
3.83
3-hydroxy-4-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
3.33
4-Hydroxy-3-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
4.33
4-hydroxy-3-methoxy-benzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30°C
8.16
4-methoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
2.93
5-(hydroxymethyl)-2-furaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
7.16 - 360
benzaldehyde
93.3
benzyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 6.5 and 65°C
405
crotonaldehyde
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55°C
101
crotonyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55°C
0.022 - 0.08
NAD+
2
NADH
Phanerochaete chrysosporium
-
pH 6.1, 30°C
0.00003 - 433
NADP+
11.33
NADPH
Phanerochaete chrysosporium
-
pH 6.1, 30°C
11.16
phenylacetaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
11.16
trans-cinnamaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0047
(R)-1-phenylethanol
Ralstonia sp.
C0IR58
wild-type, pH 8.3, 30°C
6838
0.00115 - 0.65
(S)-1-phenylethanol
4333
0.6 - 1.8
(S)-2-octanol
9492
733
3,4-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
2637
6.8
3,4-dimethoxybenzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30°C
2814
450
3,5-dimethoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
7440
2.66
3-hydroxy-4-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
19772
2.33
4-Hydroxy-3-methoxy-benzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
12368
0.166
4-hydroxy-3-methoxy-benzyl alcohol
Phanerochaete chrysosporium
-
pH 6.1, 30°C
42614
90
4-methoxybenzaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
1016
10
5-(hydroxymethyl)-2-furaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
19771
5 - 899
benzaldehyde
146
6.6
benzyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 6.5 and 65°C
260
122
crotonaldehyde
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55°C
1529
11
crotonyl alcohol
Yokenella sp. WZY002
W6CX26
at pH 8.0 and 55°C
105743
0.007 - 0.073
NAD+
7
10
NADH
Phanerochaete chrysosporium
-
pH 6.1, 30°C
8
0.0001 - 720
NADP+
10
283
NADPH
Phanerochaete chrysosporium
-
pH 6.1, 30°C
5
11.16
phenylacetaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
769
3.33
trans-cinnamaldehyde
Phanerochaete chrysosporium
-
pH 6.1, 30°C
2225
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4
3,4-dimethoxybenzaldehyde
-
pH 6.1, 30°C
165
3,4-dimethoxybenzyl alcohol
-
pH 6.1, 30°C
2.1
3,5-dimethoxybenzaldehyde
-
pH 6.1, 30°C
2.3
3-hydroxy-4-methoxy-benzaldehyde
-
pH 6.1, 30°C
5.1
4-hydroxy-3-methoxybenzaldehyde
-
pH 6.1, 30°C
136
5-(hydroxymethyl)-2-furaldehyde
-
pH 6.1, 30°C
81
benzaldehyde
-
pH 6.1, 30°C
3.5
trans-cinnamaldehyde
-
pH 6.1, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
with veratraldehyde as substrate
6.4
-
reduction activity
8
for crotonyl alcohol oxidation
8.8
-
alcohol oxidation
10.4
-
oxidation activity
11
-
for the oxidation reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
at pH 8.0 and 10.0 the enzyme retains 62% activity, while the enzyme retains 13% activity at pH 4.0
6.2 - 7.2
-
in imidazole buffer
7 - 8
-
in phosphate buffer, activity of the enzyme is only one-half that shown in imidazole buffer
7.9 - 8.5
-
in Tris buffer, activity is about 40% as in imidazole buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
isoform SDR A5
50
-
isoform SDR B3
55
for crotonyl alcohol oxidation
65
for crotonaldehyde reduction
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
by raising the temperature of the incubation mixture a linear increase of the reaction up to 55°C is noted, but the reaction over 45°C stops after 60-75 s
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
4 * 47000, SDS-PAGE
280000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
4 * 47000, SDS-PAGE
homodimer
2 * 36411, calculated from amino acid sequence; 2 * 39000, SDS-PAGE
monomer
1 * 39000, SDS-PAGE; 1 * 40580, MALDI-TOF mass spectrometry
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of the apoenzyme as well as its NADP+-bound state with resolutions down to 2.8 A. ADH displays a homotetrameric quaternary structure that can be described as a dimer of homodimers while in each subunit a seven-stranded parallel beta-sheet, flanked by three alpha-helices on each side, forms a Rossmann fold-type dinucleotide binding domain. NADP+ specificity is largely governed by the residues Asn15, Gly37, Arg38, and Arg39
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55 - 65
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C and 1.5 h at 60°C. The activity loss at 65°C is relatively rapid, and the enzyme retains 30.8% of the initial activity after 30 min of heat treatment
58
the enzyme's half-life of deactivation at 58°C is 4.2 min. There is no detectable enzyme activity when it is heated above 60°C
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DMSO
the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide and also remains active in 40% (v/v)
Ethanol
the enzyme retains more than 49% of the initial activity in 20% (v/v) ethanol and also remains active in 40% (v/v)
isopropanol
the enzyme retains more than 49% of the initial activity in 20% (v/v) isopropanol and also remains active in 40% (v/v)
Methanol
the enzyme retains more than 49% of the initial activity in 20% (v/v) methanol and also remains active in 40% (v/v)
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, potassium phosphate buffer pH 7.5, 1 mM mercaptoethanol, 2 weeks, no loss of activity
-
4°C, 10 mM Tris/HCl pH 7.5, 6 days, 95% loss of activity
-
4°C, 10 mM Tris/HCl pH 7.5, one month, 15% loss of activity
-
all buffers, standard buffer Tris-HCl , 5 mM mercaptoethanol and sucrose, 2.5 g/10 ml, in the final preparation, 2 weeks, little loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Ni-NTA column chromatography
Ni-Sepharose column chromatography
-
using Ni-NTA chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Top10 cells
-
expressed in Saccharomyces cerevisiae
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G37D
mutation in NADP+-binding site, strong decrease in activity with NADP+
N15G/G37D/R38V/R39S
mutation in NADP+-binding site, strong decrease in activity with NADP+
N15G/G37D/R38V/R39S/A86N/S88A
mutation in NADP+-binding site, strong decrease in activity with NADP+
G37D
-
mutation in NADP+-binding site, strong decrease in activity with NADP+
-
N15G/G37D/R38V/R39S
-
mutation in NADP+-binding site, strong decrease in activity with NADP+
-
N15G/G37D/R38V/R39S/A86N/S88A
-
mutation in NADP+-binding site, strong decrease in activity with NADP+
-
Show AA Sequence (304 entries)
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