Information on EC 1.1.1.90 - aryl-alcohol dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.1.1.90
-
RECOMMENDED NAME
GeneOntology No.
aryl-alcohol dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an aromatic alcohol + NAD+ = an aromatic aldehyde + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2,5-xylenol and 3,5-xylenol degradation
-
-
3-chlorotoluene degradation II
-
-
m-cresol degradation
-
-
m-xylene degradation to m-toluate
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
p-xylene degradation to p-toluate
-
-
Phenylalanine metabolism
-
-
salicin biosynthesis
-
-
salicortin biosynthesis
-
-
Toluene degradation
-
-
toluene degradation to benzoate
-
-
Tyrosine metabolism
-
-
Xylene degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
aryl-alcohol:NAD+ oxidoreductase
A group of enzymes with broad specificity towards primary alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short-chain aliphatic alcohols.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-26-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
recombinant strain H16(pBBR1-JO2ehyABcalAcalB)
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
wheatgrass
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain WCFS1
-
-
Manually annotated by BRENDA team
strain NCIMB 8826, gene gene lp_3054
-
-
Manually annotated by BRENDA team
strain NCIMB 8826, gene gene lp_3054
-
-
Manually annotated by BRENDA team
strain CBS115145
-
-
Manually annotated by BRENDA team
strain CBS115145
-
-
Manually annotated by BRENDA team
enzyme also capable of oxidizing aliphatic primary alcohols with NAD+
-
-
Manually annotated by BRENDA team
rye
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain K172
-
-
Manually annotated by BRENDA team
strain K172
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Triticale turgidocereale
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
disruption mutant shows similar growth compared to wild-type
metabolism
-
the enzyme is involved in the metabolism of benzoic acid, proteomic analysis, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-perillyl alcohol + NAD+
(R)-perillaldehyde + NADH
show the reaction diagram
(S)-perillyl alcohol + NAD+
(S)-perillaldehyde + NADH
show the reaction diagram
2,4-dihydroxy-3-(hydroxymethyl)-6-methylbenzoic acid + NAD+
3-formyl-2,4-dihydroxy-6-methylbenzoic acid + NADH
show the reaction diagram
2-aminobenzyl alcohol + NAD+
2-aminobenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
2-fluorobenzyl alcohol + NAD+
2-fluorobenzaldehyde + NADH
show the reaction diagram
2-hydroxybenzyl alcohol + NAD+
2-hydroxybenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
2-hydroxymethyl-6-methylnaphthalene + NAD+
2-formyl-6-methylnaphthalene + NADH
show the reaction diagram
2-hydroxymethylnaphthalene + NAD+
2-formylnaphthalene + NADH
show the reaction diagram
2-hydroxymethylthiophene + NAD+
2-formylthiophene + NADH
show the reaction diagram
2-methoxybenzyl alcohol + NAD+
2-methoxybenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
2-methylbenzyl alcohol + NAD+
2-methylbenzaldehyde + NADH
show the reaction diagram
3,4-dimethoxybenzyl alcohol + NAD+
3,4-dimethoxybenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
3,4-dimethylbenzyl alcohol + NAD+
3,4-dimethylbenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
3,5-dichlorobenzyl alcohol + NAD+
3,5-dichlorobenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
3-aminobenzyl alcohol + NAD+
3-aminobenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
3-chlorobenzyl alcohol + NAD+
3-chlorobenzaldehyde + NADH
show the reaction diagram
3-hydroxybenzyl alcohol + NAD+
3-hydroxybenzaldehyde + NADH
show the reaction diagram
3-methoxybenzyl alcohol + NAD+
3-methoxybenzaldehyde + NADH
show the reaction diagram
3-methyl-2-buten-1-ol + NAD+
3-methyl-2-buten-1-al + NADH
show the reaction diagram
3-methylbenzyl alcohol + NAD+
3-methylbenzaldehyde + NADH
show the reaction diagram
3-nitrobenzyl alcohol + NAD+
3-nitrobenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
4-aminobenzyl alcohol + NAD+
4-aminobenzaldehyde + NADH
show the reaction diagram
4-chlorobenzyl alcohol + NAD+
4-chlorobenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
4-ethylbenzyl alcohol + NAD+
4-ethylbenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
4-hydroxy-3-methoxybenzyl alcohol + NAD+
4-hydroxy-3-methoxybenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
4-hydroxybenzyl alcohol + NAD+
4-hydroxybenzaldehyde + NADH
show the reaction diagram
4-isopropylbenzyl alcohol + NAD+
4-isopropylbenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
4-methoxybenzyl alcohol + NAD+
4-methoxybenzaldehyde + NADH
show the reaction diagram
-
-
-
-
r
4-methylbenzyl alcohol + NAD+
4-methylbenzaldehyde + NADH
show the reaction diagram
anisaldehyde + NADH + H+
anisic alcohol + NAD+
show the reaction diagram
-
relative activity: 72.2%
-
-
r
benzaldehyde + NAD(P)H + H+
benzyl alcohol + NAD(P)+
show the reaction diagram
benzaldehyde + NADH + H+
benzyl alcohol + NAD+
show the reaction diagram
-
relative activity: 100%
-
-
r
benzaldehyde + NADPH + H+
benzyl alcohol + NADP+
show the reaction diagram
highest reductive activity with benzaldehyde
-
-
r
benzyl alcohol + NAD(P)+
benzaldehyde + NAD(P)H + H+
show the reaction diagram
benzyl alcohol + NAD+
benzaldehyde + NADH
show the reaction diagram
benzyl alcohol + NADP+
benzaldehyde + NADPH
show the reaction diagram
-
less active in the presence of NADP+ than in the presence of NAD+
-
-
?
benzyl alcohol + NADP+
benzaldehyde + NADPH + H+
show the reaction diagram
-
-
-
r
cinnamaldehyde + NADH + H+
cinnamic alcohol + NAD+
show the reaction diagram
-
relative activity: 58.7%
-
-
r
cinnamic aldehyde + NADH + H+
cinnamyl alcohol + NAD+
show the reaction diagram
cinnamyl alcohol + NAD(P)+
cinnamyl aldehyde + NAD(P)H + H+
show the reaction diagram
cinnamyl alcohol + NAD+
cinnamic aldehyde + NADH
show the reaction diagram
-
-
-
-
r
cinnamyl alcohol + NAD+
cinnamic aldehyde + NADH + H+
show the reaction diagram
cinnamyl alcohol + NAD+
cinnamyl aldehyde + NADH + H+
show the reaction diagram
-
67% of the activity with benzyl alcohol
-
-
?
cis-11-hexadecenal + NADH + H+
cis-11-hexadecenol + NAD+
show the reaction diagram
-
relative activity: 4.9%
-
-
r
citral + NADH + H+
citryl alcohol + NAD+
show the reaction diagram
-
relative activity: 7.8%
-
-
r
citronellal + NADH + H+
citronellol + NAD+
show the reaction diagram
-
relative activity: 21.8%
-
-
r
coniferyl alcohol + NAD(P)+
coniferyl aldehyde + NAD(P)H + H+
show the reaction diagram
coniferyl alcohol + NAD+
coniferyl aldehyde + NADH + H+
show the reaction diagram
coniferyl aldehyde + NADH + H+
coniferyl alcohol + NAD+
show the reaction diagram
crotonaldehyde + NADPH + H+
crotonyl alcohol + NADP+
show the reaction diagram
-
-
-
r
crotonyl alcohol + NADP+
crotonaldehyde + NADPH + H+
show the reaction diagram
highest oxidative activity with crotyl alcohol
-
-
r
decanal + NADH + H+
decanol + NAD+
show the reaction diagram
-
relative activity: 19.7%
-
-
r
dodecanal + NADH + H+
dodecanol + NAD+
show the reaction diagram
-
relative activity: 8.9%
-
-
r
furan-2-methanol + NAD+
2-formylfuran + NADH
show the reaction diagram
-
-
-
-
r
furfuryl alcohol + NAD+
furfural + NADH
show the reaction diagram
-
-
-
-
r
geranial + NADH + H+
geraniol + NAD+
show the reaction diagram
-
-
-
-
r
geraniol + NAD(P)+
geranial + NAD(P)H + H+
show the reaction diagram
geraniol + NAD+
(2Z)-3,7-dimethylocta-2,6-dienal + NADH + H+
show the reaction diagram
-
105% of the activity with benzyl alcohol
-
-
?
geraniol + NAD+
geranial + NADH
show the reaction diagram
glutaraldehyde + NADH + H+
? + NAD+
show the reaction diagram
-
relative activity: 0.6%
-
-
r
glyoxal + NADH + H+
? + NAD+
show the reaction diagram
-
relative activity: 0.7%
-
-
r
hexanal + NADH + H+
hexanol + NAD+
show the reaction diagram
-
relative activity: 6.4%
-
-
r
hydroxybenzyl alcohol + NAD+
hydroxybenzaldehyde + NADH
show the reaction diagram
-
-
-
?
methoxybenzyl alcohol + NAD+
methoxybenzaldehyde + NADH
show the reaction diagram
-
-
-
?
methylbenzyl alcohol + NAD+
methylbenzaldehyde + NADH
show the reaction diagram
-
-
-
?
nerol + NAD(P)+
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
show the reaction diagram
nerol + NAD+
(2E)-3,7-dimethylocta-2,6-dienal + NADH + H+
show the reaction diagram
-
116% of the activity with benzyl alcohol
-
-
?
nerol + NAD+
neral + NADH
show the reaction diagram
octanal + NADH + H+
octanol + NAD+
show the reaction diagram
-
relative activity: 36.5%
-
-
r
perillyl alcohol + NAD+
perillaldehyde + NADH
show the reaction diagram
-
-
-
-
r
phenethyl alcohol + NAD(P)+
phenylacetaldehyde + NAD(P)H + H+
show the reaction diagram
phenethyl alcohol + NAD+
phenylacetaldehyde + NADH + H+
show the reaction diagram
-
73% of the activity with benzyl alcohol
-
-
?
salicyl aldehyde + NADH + H+
salicyl alcohol + NAD+
show the reaction diagram
-
relative activity: 9%
-
-
r
vanillin + NADH + H+
vanillin alcohol + NAD+
show the reaction diagram
-
relative activity: 4%
-
-
r
vanillyl alcohol + NAD+
vanillyl aldehyde + NADH + H+
show the reaction diagram
-
-
-
?
xylene-2,2'-diol + NAD+
? + NADH
show the reaction diagram
low activity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,4-dihydroxy-3-(hydroxymethyl)-6-methylbenzoic acid + NAD+
3-formyl-2,4-dihydroxy-6-methylbenzoic acid + NADH
show the reaction diagram
-
the enzyme plays a role in the atranorin pathway
-
-
?
2-hydroxymethyl-6-methylnaphthalene + NAD+
2-formyl-6-methylnaphthalene + NADH
show the reaction diagram
2-hydroxymethylnaphthalene + NAD+
2-formylnaphthalene + NADH
show the reaction diagram
3-chlorobenzyl alcohol + NAD+
3-chlorobenzaldehyde + NADH
show the reaction diagram
benzaldehyde + NAD(P)H + H+
benzyl alcohol + NAD(P)+
show the reaction diagram
benzyl alcohol + NAD(P)+
benzaldehyde + NAD(P)H + H+
show the reaction diagram
benzyl alcohol + NAD+
benzaldehyde + NADH
show the reaction diagram
cinnamyl alcohol + NAD(P)+
cinnamyl aldehyde + NAD(P)H + H+
show the reaction diagram
coniferyl alcohol + NAD(P)+
coniferyl aldehyde + NAD(P)H + H+
show the reaction diagram
geraniol + NAD(P)+
geranial + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
hydroxybenzyl alcohol + NAD+
hydroxybenzaldehyde + NADH
show the reaction diagram
Q2Z1W3, Q2Z1W4, Q2Z1W5
-
-
-
?
methoxybenzyl alcohol + NAD+
methoxybenzaldehyde + NADH
show the reaction diagram
Q2Z1W3, Q2Z1W4, Q2Z1W5
-
-
-
?
methylbenzyl alcohol + NAD+
methylbenzaldehyde + NADH
show the reaction diagram
Q2Z1W3, Q2Z1W4, Q2Z1W5
-
-
-
?
nerol + NAD(P)+
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
phenethyl alcohol + NAD(P)+
phenylacetaldehyde + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
vanillyl alcohol + NAD+
vanillyl aldehyde + NADH + H+
show the reaction diagram
Q2Z1W3, Q2Z1W4, Q2Z1W5
-
-
-
?
xylene-2,2'-diol + NAD+
? + NADH
show the reaction diagram
Q2Z1W3, Q2Z1W4, Q2Z1W5
low activity
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ag+
-
0.01 mM, residual activity: 0.2%
Cu2+
-
0.1 mM, residual activity: 1.3%
Fe2+
-
1 mM, residual activity: 21.7%
Hg+
-
0.001 mM, residual activity: 0.1%
Mn2+
-
1 mM, activity enhanced to 128%
Zn
-
zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved
additional information
-
no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-Bromobenzaldehyde
-
0.01 mM, 88% inhibition
2-Chlorobenzaldehyde
-
0.01 mM, 95% inhibition
2-Fluorobenzaldehyde
-
0.1 mM, 100% inhibition
2-mercaptoethanol
3,4-dimethoxybenzaldehyde
-
0.1 mM, 10% inhibition
3-methylbenzaldehyde
-
0.1 mM, 75% inhibition
4-Isopropylbenzaldehyde
-
0.01 mM, 87% inhibition
5,5'-dithiobis-(2-nitrobenzoate)
-
-
AgNO3
dithiothreitol
-
above 2.5 mM
Fe3+
1 mM, 30% residual activity
iodoacetamide
iodoacetic acid
1 mM, 57% residual activity
N-bromosuccinimide
1 mM, 3% residual activity
N-ethylmaleimide
NADH
-
noncompetitive
p-chloromercuribenzoate
pentafluorobenzaldehyde
-
0.1 mM, 44% inhibition
SH-blocking agents
-
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
application of vanillin to the mycelium leads to up-regulation of enzymes involved in vanillin metabolism and also in glycolysis, the tricarboxylic acid cycle, the pentose-phosphate cycle, and heme biosynthesis, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
2-hydroxybenzyl alcohol
-
-
0.111
2-Methoxybenzyl alcohol
-
-
0.605 - 0.992
2-Methylbenzyl alcohol
0.053
3,4-dimethoxybenzyl alcohol
-
-
0.145
3-Methoxybenzyl alcohol
-
-
0.017 - 0.11
3-methyl-2-buten-1-ol
0.081 - 0.146
3-methylbenzyl alcohol
0.046 - 0.3
4-Hydroxybenzyl alcohol
0.111
4-Isopropylbenzyl alcohol
-
-
0.057
4-methoxybenzyl alcohol
-
-
0.106 - 0.118
4-methylbenzyl alcohol
0.0257 - 0.28
benzaldehyde
0.0173 - 0.32
benzyl alcohol
0.093 - 1.7
cinnamyl alcohol
0.044 - 0.13
coniferyl alcohol
5.1
coniferyl aldehyde
pH 8.0, 30°C
0.0152 - 0.4
NAD+
0.017 - 1.49
NADH
0.018
perillyl alcohol
-
-
0.111
thiophen-2-methanol
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
156
3-Methoxybenzyl alcohol
Acinetobacter calcoaceticus
-
-
37 - 41
3-methyl-2-buten-1-ol
80
4-Hydroxybenzyl alcohol
Acinetobacter calcoaceticus
-
-
144
4-Isopropylbenzyl alcohol
Acinetobacter calcoaceticus
-
-
116
4-methoxybenzyl alcohol
Acinetobacter calcoaceticus
-
-
622 - 12940
benzaldehyde
3.4 - 228
benzyl alcohol
116 - 141
cinnamyl alcohol
69.7
coniferyl alcohol
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
354
coniferyl aldehyde
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
53.7
NAD+
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
8920
NADH
Acinetobacter baylyi
-
pH 7.4, 30°C
83
perillyl alcohol
Acinetobacter calcoaceticus
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
46700
benzaldehyde
Acinetobacter baylyi
-
pH 7.4, 30°C
146
68.3
cinnamyl alcohol
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
936
536
coniferyl alcohol
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
1202
69.3
coniferyl aldehyde
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
1677
134
NAD+
Streptomyces sp.
M5AJW4
pH 8.0, 30°C
7
528000
NADH
Acinetobacter baylyi
-
pH 7.4, 30°C
8
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
NADH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
biofilter culture cell extract, cofactor NADP+
0.016
-
liquid culture cell extract, cofactor NAD+
0.036
-
biofilter culture cell extract, cofactor NAD+
0.05
-
liquid culture cell extract, cofactor NADP+
0.106
-
-
0.22
-
recombinant strain
0.24
-
comparison to recombinant enzymes
20.31 - 58.7
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
benzaldehyde + NADH
6
-
reduction of benzaldehyde
7.5
-
assay at
8
for crotonyl alcohol oxidation
8.9
-
benzaldehyde + NADH
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 6
-
pH 3.5: about 60% of maximal activity, pH 6.0: about 45% of maximal activity
4.7 - 7.6
-
less than half-maximal activity above and below, reduction of benzaldehyde
7.5 - 10.5
-
pH 7.5: about 15% of activity maximum, pH 10.5: about 20% of activity maximum
8.5 - 9.7
-
less than half-maximal activity above and below, oxidation of benzyl alcohol
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
55
for crotonyl alcohol oxidation
65
for crotonaldehyde reduction
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
-
20°C: about 60% of maximal activity, 50°C: about 70% of maximal activity
25 - 50
-
activity range
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.04
-
sequence calculation
6.7
-
sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
gel filtration
37598
4 * 37598, MALDI-TOF
38519
2 * 38519, sequence of cDNA
39500
-
SDS-PAGE
39700
-
4 * 39700, SDS-PAGE, cross-linking experiments in vitro using dimethylsuberimidate
42000
-
2 * 42000, SDS-PAGE
72600
-
gel filtration
75000
-
gel filtration, p-cresol grown cells
82000
-
gel filtration
90000
-
gel filtration
122000
-
gel filtration, 3,5-xylenol grown cells, activity 2
145000
-
gel filtration, 3,5-xylenol grown cells, activity 1
151000
gel filtration
155000
-
gel filtration
160000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
-
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.5
75 h, more than 80% of initial activity
724592
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
enzyme reaches 52% of its maximum activity at 15°C
30 - 50
-
the purified recombinant enzyme remains quite stable
37
-
after 21 h no loss of activity
50
pH 7.0, stable up to
55
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C
56
-
after 10 min complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol stabilizes
-
benzyl alcohol stabilizes slightly
-
dithiothreitol stabilizes
-
MgCl2 stabilizes slightly
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DMSO
the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, concentrated, mixed with ethanediol
-
-70°C, enzyme dialyzed against buffer containing 40% glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme also capable of oxidizing aliphatic primary alcohols with NAD+
-
enzyme encoded by plasmid TOL
-
ion-exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, affinity chromatography
-
partially, subcellular fractionation
-
recombinant lp_3054 from Escherichia coli strain JM109
-
two distinct NAD+-dependent m-hydroxybenzyl alcohol dehydrogenases from cells grown on 3,5-xylenol, differing in relative rates with the various substrates, another NAD+-dependent alcohol dehydrogenase, not identical with the former ones from p-cresol-grown cells
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Escherichia coli XL1-Blue
-
gene adhA, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Escherichia coli; gene adhB, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Escherichia coli; gene adhC, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Escherichia coli
gene lp_3054, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H47Q
-
increased Km for benzyl alcohol and benzaldehyde, decreased Km for NAD+ and NADH
H47Q/V61H
-
unchanged Km for benzyl alcohol, decreased Km for NAD+ and benzaldehyde
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
biotechnological production of vanillin
degradation
synthesis
-
biotechnological production of vanillin
Show AA Sequence (363 entries)
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