Information on EC 1.1.1.84 - dimethylmalate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.84
-
RECOMMENDED NAME
GeneOntology No.
dimethylmalate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-3,3-dimethylmalate + NAD+ = 3-methyl-2-oxobutanoate + CO2 + NADH
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
redox reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pantothenate and CoA biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
(R)-3,3-dimethylmalate:NAD+ oxidoreductase (decarboxylating)
Requires K+ or NH4+ and Mn2+ or Co2+; also acts on (R)-malate.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-21-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
P-2
-
-
Manually annotated by BRENDA team
P-2
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-3,3-dimethylmalate + NAD+
3-methyl-2-oxobutanoate + CO2 + NADH
show the reaction diagram
3-(n-propyl)malate + NAD+
2-oxopentanoate + CO2 + NADH
show the reaction diagram
D-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(R)-3,3-dimethylmalate + NAD+
3-methyl-2-oxobutanoate + CO2 + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
Mn2+ or Co2+ required
K+
-
K+ or NH4+ required
Mn2+
-
Mn2+ or Co2+ required
NH4+
-
K+ or NH4+ required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-Butanedione
-
-
additional information
-
fairly stable against SH-group reactants
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15 - 0.2
3,3-dimethyl-D-malate
0.33
3-(n-Propyl)malate
-
-
8.9 - 11
D-malate
0.27
NAD+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
-
substrates 3,3-dimethylmalate + NAD+
8.4
-
substrates 3,3-dimethylmalate + NAD+
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
136000
-
ultracentrifugation
142000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
5 min, 40% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
50 days, 0°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE